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Use of Calcium Phosphate Cement for Repairing Bone Defects

Histomorphometric and Immunohistochemical Analyses

Gulinelli, Jéssica Lemos, PhD*; Queiroz, Thallita Pereira, PhD; Hochuli-Vieira, Eduardo, PhD; Okamoto, Roberta, PhD§; Mattos, João Marcos Borges, PhD*; Calcagnotto, Thiago, PhD||; Santos, Pâmela Leticia dos, PhD

doi: 10.1097/SCS.0000000000005526
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This study aimed to assess the repair of surgically created bone defects filled with blood clot, autogenous bone, and calcium phosphate cement, by histomorphometric and immunohistochemical analyses. Ten adult male rabbits were used. Three bone defects were prepared with an 8-mm diameter trephine bur in the parietal region of each animal and filled with blood clot (Group BC), autogenous bone (Group AB), and calcium phosphate bone cement (Group CPC). The animals were euthanized at 40 and 90 postoperative days. The sections were subjected to histomorphometric analysis of the new bone formed inside the calvarial defects and immunohistochemical staining to determine the expression of osteocalcin (OC), osteopontin (OP), and tartrate-resistant acid phosphatase (TRAP) proteins. Histomorphometric data were analyzed statistically by analysis of variance and Tukey's post hoc test at 5% significance level. In the results at 40 and 90 days, Group AB differed significantly from Group CPC regarding the area of newly formed bone. The immunohistochemical analysis revealed expression of OP, OC, and TRAP proteins in all groups. Group AB showed prevalence of OC and OP, and lower TRAP expression. Therefore, the calcium phosphate bone cement assessed in the present study did not accelerate the protein expression dynamics during bone healing, compared with the autogenous group.

*Private Clinic

Department of Surgery, University of Araraquara – UNIARA

Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery, School of Araraquara, UNESP

§Department of Basic Science, Division of Anatomy, School of Araçatuba, UNESP

||Department of Research and Post-Graduates, University of Sacred Heart, São Paulo, Brazil.

Address correspondence and reprint requests to Pâmela Leticia dos Santos, PhD, Department of Health Sciences, University of Araraquara (UNIARA), Rua Carlos Gomes, 1338, Centro, Araraquara-SP 14801-340, Brazil; E-mail: pamelalsantos@hotmail.com

Received 30 July, 2018

Accepted 4 March, 2019

The authors report no conflicts of interest.

© 2019 by Mutaz B. Habal, MD.