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Evaluation of New Bone Formation Using Autogenous Tooth Bone Graft Combined with Platelet-Rich Fibrin in Calvarial Defects

Kizildağ, Alper, PhD*; Taşdemir, Ufuk, PhD; Arabaci, Taner, PhD; Özmen, Özlem, PhD§; Kizildağ, Canan Aksu, PhD||; Iyilikci, Berrin, PhD

doi: 10.1097/SCS.0000000000005413
Original Article: PDF Only

The purpose of the present study was to evaluate the contributions of autogenous tooth bone graft (ATBG) combined with platelet-rich fibrin (PRF) on new bone formation and bone morphogenetic protein (BMP)-2 in rabbit calvarial defects. Twelve male New Zealand rabbits were used in this study. Three circular bone defects were prepared in each rabbit with a drill. These defects were divided into 3 groups: control, treated with ATBG, and treated with ATBG+PRF. The animals were sacrificed at 28 days. Samples were evaluated by histomorphometric analyses and total augmented area, new bone area and bone density were calculated. In addition, expression of BMP-2 was determined by immunohistochemical staining. The total augmented area, new bone area and bone density were significantly greater in the ATBG group than in the control group (P <0.05). Also, these values were significantly higher in the ATBG+PRF group than the ATBG group (P <0.05). Test groups demonstrated significantly increased BMP-2 levels compared with the control group (P <0.05). The present study suggested that ATBG combined with PRF significantly increased the new bone formation and enhanced bone healing in cranial defects.

*Department of Periodontology

Department of Maxillofacial Surgery, Faculty of Dentistry, Pamukkale University, Denizli

Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum

§Department of Pathology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur

||Department of Orthodontics, Faculty of Dentistry, Pamukkale University, Denizli, Turkey.

Address correspondence and reprint requests to Dr Alper Kizildağ, PhD, Pamukkale University, Faculty of Dentistry, Department of Periodontology 20070, Denizli, Turkey; E-mail:

Received 30 October, 2018

Accepted 15 January, 2019

The authors report no conflicts of interest related to this study. This study was self-funded by the authors.

© 2019 by Mutaz B. Habal, MD.