In our study, the authors aimed to obtain a live and functional sinus epithelium with mesenchymal stem cells and nasal mucosa epithelial cells from rabbits which are cultured in temperature-responsive culture plates to get a single-layer.
Twenty-two female New Zealand rabbits were included in the study. Two of them were used to obtain mesenchymal stem cells. A total of 40 maxillary sinuses were randomly divided into 5 groups: 1) control group which is used to investigate normal rabbit maxillary mucosa, 2) secondary healing group, 3) mesenchymal stem cell graft group, 4) differentiated mesenchymal stem cell group, and 5) nasal mucosal graft group. The animals were sacrificed at the 28th day after the surgery.
Scanning electron microscopy, transmission electron microscopy, and immunohistochemical investigations were performed.
With these investigations, it was shown that; all graft groups were histologically better than secondary healing group and when the authors compared the graft groups, differentiated mesenchymal stem cell group were the best.
Our study results showed that endoscopic sinus surgery and treatment with cell sheets, which were generated in temperature-responsive culture dishes, had more functional respiratory epithelium.
*Department of Otorhinolaryngology, Acibadem University Faculty of Medicine
†Stem Cell and Gene Therapy Research and Applied Center, Liv Hospital, Istanbul
‡Department of Otorhinolaryngology, Sakarya University Faculty of Medicine, Sakarya
§Center for Stem Cell and Gene Therapies Research and Practice, Institude of Health Sciences, Stem Cell Department, Kocaeli University, Kocaeli
||Department of Histology and Embryology, Akdeniz University Faculty of Medicine, Antalya
¶Department of Otorhinolaryngology, Kocaeli University Faculty of Medicine
#Department of Otorhinolaryngology, Kocaeli Medical Park Hospital, Kocaeli, Turkey.
Address correspondence and reprint requests to Ahmet Kara, MD, Sakarya University Training and Research Hospital, 54000, Korucuk, Sakarya, Turkey; E-mail: email@example.com
Received 19 February, 2019
Accepted 23 April, 2019
This study has been funded by the Turkey Scientific and Technological Research Center (TUBITAK project number: 113S871). The research protocol was approved by the Kocaeli University Animal Ethics Committee (KOU HADYEK3/7 2013).
This was presented at 15th Turkish Rhinology, 7th National Otology-Neurology, and National Head and Neck Congress, April 4–7, 2019; Ankara, Turkey.
The authors report no conflicts of interest.