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Effects of Different Levels of Restraint Stress on Bone–Implant Contact

Bozoglan, Alihan, DDS, PhD*; Dundar, Serkan, DDS, PhD*; Yildirim, Tuba T., DDS, PhD*; Bulmus, Ozgur, DVM, PhD; Ertugrul, Abdullah Seckin, DDS, PhD; Bozoglan, Merve Y., MD§; Tekin, Samet, DDS, MSC||; Toy, Vesile Elif, DDS, PhD

Journal of Craniofacial Surgery: June 2019 - Volume 30 - Issue 4 - p 1294–1297
doi: 10.1097/SCS.0000000000005104
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This study examined the effects of different levels of chronic restraint stress on bone–titanium implant contact in rats. This study included 32 adult female Sprague–Dawley rats. The machined surface titanium implants were surgically placed into the metaphyseal region of the rat tibias. Next, the rats were divided randomly into 4 groups, namely, control (CNT) (n = 8), low-restraint stress (LRS) (n = 8), medium-restraint stress (MRS) (n = 8), and high-restraint stress (HRS) (n = 8) groups. The rats in the CNT group received only the titanium implants surgically but did not receive any further treatment during the experimental period of 30 days. The rats in the LRS, MRS, and HRS groups were applied restraint stress for 1, 2, and 4 hours, respectively, daily for 28 days starting from day 2 after the surgery. At the end of the study period, the rats were sacrificed and their implants and the surrounding bone tissues were harvested for performing nondecalcified histological analysis. Moreover, blood samples were collected from the rats and were centrifuged for analyzing serum cortisol levels. Serum cortisol levels of the rats in the LRS, MRS, and HRS groups were higher than those of the rats in the CNT group (P < 0.05). Moreover, serum cortisol levels of the rats in the HRS group were higher than those of the rats in the MRS and LRS groups (P < 0.05). The extent of bone–implant contact was lower in the rats in the HRS group than in the rats in the CNT and LRS groups (P < 0.05). These data suggest that the application of 4-hour chronic restraint stress during the 28-day experimental period impaired the bone–implant contact.

*Department of Periodontology, Faculty of Dentistry

Department of Physiology, Faculty of Health Sciences, Firat University, Elazig

Department of Periodontology, Faculty of Dentistry, Izmir Katip Çelebi University, Izmir

§Department of Pharmacology, Faculty of Medicine

||Department of Prosthetics, Faculty of Dentistry, Firat University, Elazig

Department of Periodontology, Faculty of Dentistry, Inonu University, Malatya, Turkey.

Address correspondence and reprint requests to Serkan Dundar, DDS, PhD, Department of Periodontology, Faculty of Dentistry, Firat University, 23119, Campus, Elazig, Turkey; E-mail: sdundar@firat.edu.tr, dtserkandundar@gmail.com

Received 6 July, 2018

Accepted 10 September, 2018

This study was presented as an oral presentation in 23 International Scientific Congress of Turkish Dental Association, September 21–24, 2017, Istanbul, Turkey.

The authors report no conflicts of interest.

© 2019 by Mutaz B. Habal, MD.