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Radiological and Stereological Evaluation of the Effect of Rifampin on Bone Healing in Critical-Size Defects

Durmuşlar, Mustafa Cenk DDS, PhD; Balli, Umut; Türer, Akif; Önger, Mehmet E.; Çelik, Hakan H.

doi: 10.1097/SCS.0000000000002762
Original Articles
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Objectives: This study aimed to investigate the effect of rifampin with autogenous bone on bone regeneration in critical-size defects in the calvaria of rats.

Materials and Methods: In total, 40 rats were divided into 4 groups and a 5-mm diameter of calvarial defect was made in each rat's calvarium. Control group (C), bone defects were irrigated with sterile saline; rifampin group (R), bone defects were irrigated with rifampin. In the autogenous graft group (Ag), the autogenous graft was contaminated with saliva, and the defects were filled with an autogenous graft. In the autogenous graft + rifampin group (Ag+R), the autogenous graft was contaminated with saliva and was decontaminated with rifampin, and the defects were filled with the autogenous graft. The animals were killed at 4weeks. Bone formation was assessed by micro-computed tomography scanning and stereological analyses.

Results: The mean new bone volume was the greatest in the Ag/rifampin group (1.73 ± 0.17), followed by the Ag group (1.50 ± 0.05) (statistically significant difference at P < 0.05). The new bone volume was the lowest in the control group (1.05 ± 0.09); however, no difference was observed compared with the rifampin group (1.08 ± 0.07) (P > 0.05).

Conclusion: This study, despite its limitations, showed that rifampin with autogenous bone increased bone regeneration in rats with critical-size defects.

*Department of Oral and Maxillofacial Surgery

Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak

Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayis University, Samsun

§Department of Anatomy, Faculty of Medicine, Hacettepe University, Ankara, Turkey.

Address correspondence and reprint requests to Mustafa Cenk Durmuşlar, DDS, PhD, Bulent Ecevit Universitesi Dishekimligi Fakultesi, Agiz Dis Cene Hastaliklari ve Cerrahisi Anabilim Dali, Kozlu, Zonguldak, Turkey; E-mail: cenkdurmuslar@hotmail.com

Received 9 February, 2016

Accepted 10 April, 2016

The authors report no conflicts of interest.

© 2016 by Mutaz B. Habal, MD.