Scientific FoundationsComparison of the Effect of Platelet-Rich Plasma and Simvastatin on Healing of Critical-Size Calvarial Bone DefectsSeyhan, Nevra MD; Keskin, Suat MD; Aktan, Murat MD; Avunduk, Mustafa Cihad MD; Sengelen, Meltem MD; Savaci, Nedim MD Author Information *Polatli State Hospital, Ankara †Department of Radiology ‡Department of Histology §Department of Pathology, Meram Medical School, Necmettin Erbakan University, Konya ||Department of Public Health, Hacettepe University, Ankara ¶Department of Plastic and Reconstructive Surgery, Meram Medical School, Necmettin Erbakan University, Konya, Turkey. Address correspondence and reprint requests to Nevra Seyhan, MD, Plastic Surgery Clinic, Polatli State Hospital, 06900 Ankara, Turkey; E-mail: [email protected] Received 10 July, 2015 Accepted 8 March, 2016 The authors report no conflicts of interest. Journal of Craniofacial Surgery: July 2016 - Volume 27 - Issue 5 - p 1367-1370 doi: 10.1097/SCS.0000000000002728 Buy Metrics Abstract Objective: In previous studies, it has been shown that both simvastatin (chlolesterollowering drug) and platelet-rich plasma (PRP) were capable of promoting bone formation. The aim of this study was to compare the effects of PRP and simvastatin on healing of critical-size bone defects. Methods: A total of 33 rats (3 for PRP preparation) were used in the experiment. Critical-size defect 8-mm diameter was created in 30 rats’ calvarium. Rats were divided into 3 groups. Each group contained 10 animals. In Group A the defect was filled with phosphate-buffered saline only, in Group B with 0.5 mL PRP, and in Group C with 0.1 mg simvastatin. The defects were evaluated by radiographic analysis at 8th and 16th weeks. The animals were sacrificed 16 weeks after the surgery. Histological examination was performed to assess the new bone-forming area. Vessels, fibroblasts, osteoblasts, and osteoclasts were marked in 524749.1-μm2 area and counted with using Clemex Vision Lite 3.5 Image Analysis program. The results were statistically analyzed. © 2016 by Mutaz B. Habal, MD.