Secondary Logo

Journal Logo

Role of prognostic biomarker decoy receptor 3 and immunomodulation in kidney diseases

Weng, Shuo-Chuna,b; Tarng, Der-Chernga,c,d,*

Journal of the Chinese Medical Association: September 2019 - Volume 82 - Issue 9 - p 680–684
doi: 10.1097/JCMA.0000000000000149
Review Article
Open

Decoy receptor 3 (DcR3), also known as tumor necrosis factor receptor superfamily member 6b (TNFRSF6B), was recently identified as a novel biomarker for predicting progression of kidney diseases with potential immune modulation. The purpose of this review is to discuss the current evidence related to DcR3 in kidney diseases and to compare the differences between human and animal studies both in vivo and in vitro. High serum DcR3 predicts the occurrence of peritonitis in patients receiving chronic peritoneal dialysis and is positively correlated with inflammatory markers such as interleukin-6, high-sensitivity C-reactive protein, and adhesion molecules in patients on maintenance hemodialysis (HD). Higher serum DcR3 levels not only independently predict cardiovascular and all-cause mortality in HD patients but also identify older adults on HD at risk of protein-energy wasting in combination with a low geriatric nutritional risk index. Recently, renal tubular epithelial cells (RTECs) expressing DcR3 have also been used to predict progression of chronic kidney disease. Expression of DcR3 was correlated with a 2-fold increase in serum creatinine or failure of kidney allograft. DcR3 could protect renal myofibroblasts against Fas-induced apoptosis and subsequently lead to renal fibrosis. Locally expressed DcR3 in the RTECs may suppress the FasL-Fas-mediated apoptosis of T cells, resulting in an accumulation of allo-reactive T cells. In addition to traditional biological functions, recombinant DcR3.Fc and cytomegalovirus promoter-driven human DcR3 plasmid are able to modulate the activation and differentiation of dendritic cells and macrophages via “non-decoy” action. Both progressive IgA nephropathy and autoimmune crescentic glomerulonephritis in mice can be suppressed after hydrodynamics-based gene delivery of DcR3 plasmid. DcR3-mediated effects in vitro could be surveyed via over-expressing DcR3 or addition of recombinant DcR3.Fc, and CD68-driven DcR3 transgenic mice are suitable for investigating systemic effect in vivo. Inhibition of DcR3 expression in human may be a promising approach for pathomechanism.

aInstitute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan, ROC

bCenter for Geriatrics and Gerontology, Division of Nephrology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan, ROC

cDepartment and Institute of Physiology, National Yang-Ming University, Taipei, Taiwan, ROC

dDivision of Nephrology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, ROC

Received June 18, 2019; accepted June 19, 2019.

Conflicts of interest: The authors declare that they have no conflicts of interest related to the subjects matter or materials discussed in this article.

Address correspondence: Dr. Der-Cherng Tarng, Division of Nephrology, Department of Medicine, Taipei Veterans General Hospital, 201, Section 2, Shi-Pai Road, Taipei 112, Taiwan, ROC. E-mail address: dctarng@vghtpe.gov.tw (D.-C. Tarng).

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Back to Top | Article Outline

1. INTRODUCTION

Chronic kidney disease (CKD) has emerged as an important public health burden in Taiwan, and cardiovascular disease (CVD) and malnutrition-inflammation-cachexia were the leading causes of both morbidity and mortality in patients with CKD, hemodialysis (HD), and peritoneal dialysis (PD).1 Decoy receptor 3 (DcR3) has emerged as a novel pleiotropic immunomodulator to modulate proinflammatory responses via “decoy” and “non-decoy” actions,2,3 and thus may serve as a biomarker of disease severity or a predictor of disease outcomes. DcR3 is not detectable in most normal human kidney tissues, but serum and tissue DcR3 levels are higher in patients with late-stage CKD, especially in HD patients, when compared with those in cancer patients or normal individuals.4,5 Our previous study determined that DcR3 not only represents sustained low-grade systemic inflammation in CKD patients,6 but also indicates subsequent residual inflammation in localized kidney allograft and further allograft fibrosis.7 Systemic inflammation is especially high in PD and HD patients.8,9 Furthermore, DcR3 might serve as a marker for malnutrition-inflammation status. Serum DcR3 was inversely related to albumin and positively related to high-sensitivity C-reactive protein (hs-CRP) and low nutritional status.10

In the tumor necrosis factor receptor (TNFR) family, DcR3 is the only member capable of neutralizing three ligands: Fas ligand (FasL), LIGHT (homologous to lymphotoxin, exhibits inducible expression and competes with HSV glycoprotein D for binding to herpesvirus entry mediator, a receptor expressed on T lymphocytes), and tumor necrosis factor-like ligand 1A (TL1A). From this perspective, DcR3.Fc-mediated anti-inflammation involves TNFRSF6B/DcR3-induced T-cell costimulation, which possibly operates through FasL-mediated reverse signaling.11 However, inflammation is also down-regulated due to DcR3-induced M2/Th2-like phenotype via activation of heparin sulfate proteoglycans, such as syndecan-2 and CD44v3.12 In basic studies, in addition to “decoy” function, DcR3 can suppress the Th1 response and attenuate cell-mediated immunity in vitro.13,14 The recombinant DcR3.Fc fusion protein is able to induce CD14+-monocyte differentiation into CD1alowCD40lowCD54lowCD80lowCD86high dendritic cells (DCs), which then skew T cell differentiation into the Th2 phenotype.3,13,14 Hsieh et al also found DcR3 modulates macrophage activation toward an M2-like phenotype in vitro and that DcR3 downregulates MHC class II expression in tumor-associated macrophages (TAMs) via epigenetic control.12,15 Compared with wild-type CT26 colon cancer cells, enhanced migration and invasion have been observed in CT26-DcR3 stable transfectants. In an animal model, compared with wild-type mice, significantly enhanced tumor growth and spreading were observed in CD68 promoter-driven DcR3 transgenic (Tg) mice.16 Therefore, DcR3.Fc-treated DCs skew T cell differentiation into the Th2 phenotype, while DcR3.Fc-treated macrophages behave in the same manner as the M2 phenotype.

Based on the current available evidence, the precise roles of DcR3 in kidney diseases remain unclear and thus further study is needed to elucidate the underlying mechanisms.

Back to Top | Article Outline

2. PURPOSE OF THE REVIEW

With respect to candidate biomarkers in the progression of CKD itself and outcomes of CKD patients, there are currently no identical and representative biomarkers, which carry both immunological and nonimmunological factors. Accordingly, traditional methods, such as baseline estimated glomerular filtration rate (eGFR) by category, absolute change in eGFR value, annual eGFR decline or annual percentage change of eGFR, velocity of eGFR slopes, and eGFR variability, have been widely used for predicting cognitive deterioration, cardiovascular risk, renal outcome, and patient mortality.17–23 The other established risk factors to predict progression of CKD, survival of kidney allograft, and outcomes of patients are diabetes mellitus, hypertension, hyperlipidemia, hyperuricemia, proteinuria, and Charlson Cormobidity Index score.24–26 Specific risk factors for survival of kidney allograft and renal transplant recipients (RTRs) are posttransplantation diabetes mellitus (PTDM),27 metabolic syndrome,28 posttransplantation glomerulonephritis (PTGN),29 sarcopenia,30 and recurrent kidney allograft rejection.7

Several candidate biomarkers such as longitudinal measurements of cystatin C,18 urine neutrophil gelatinase-associated lipocalin (NGAL),31 serum cytokines, chemokines, and microRNAs32 are now available. However, there is some controversy in the literature. Serum or tissue DcR3 is not inferior to the diagnostic performance of several available tests, and serves as an adequate biomarker in terms of accuracy of risk prediction.6–10 However, DcR3 is not found in mouse and rat genomes.2,3 The purpose of the current review includes the comparison of the difference between human and animal studies. Overexpression of DcR3 in Tg mice model or hydrodynamics-based gene delivery of DcR3 plasmid could mimic pathophysiologic expression in human after acute or chronic insults. The real pathomechanistic roles for upregulation of DcR3 during inflammation inducing positive or negative-feedback reaction in patients with CKD, HD, PD, or kidney transplantation may be performed in the inhibition of DcR3 expression in human primary cells or transformed cells.

Back to Top | Article Outline

3. SCIENTIFIC EVIDENCE

3.1. Serum DcR3 as a biomarker for inflammation and patient outcomes

We found that high serum DcR3 levels were associated with occurrence of PD-related peritonitis in patients receiving chronic PD. Baseline serum DcR3 in PD patients was 1.94 ± 1.23 ng/mL.8 However, relatively high serum DcR3 concentrations ranged from 0.05 to 17.78 ng/mL in maintenance HD patients.9 Baseline serum DcR3 showed a strong positive correlation with inflammatory markers (interleukin-6 [IL-6] and hs-CRP) and adhesion molecules, including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1).9 Low serum albumin and history of CVD, the leading two causes of morbidity in patients on HD, were significantly and negatively associated with DcR3 levels.9 More importantly, serum DcR3 levels were elevated in HD patients and were closely related to inflammation, cardiovascular, and all-cause mortality.9 The potential mechanism involves DcR3 modulating the proinflammatory response via “non-decoy” activities through NF-κB-mediated expression of ICAM-1, VCAM-1, and IL-8 by monocytes, whose binding capacity to endothelium was shown to be enhanced in circulation.33

Serum DcR3 level in RTRs was relatively low when compared with the levels in mice treated with hDcR3 or Tg overexpression (150 to 850 ng/mL).12,34 RTRs in the high DcR3 expression (HDE) in RTECs had high serum DcR3 levels (1.52 ± 0.36 ng/mL) compared with the levels (0.71 ± 0.27 ng/mL) of the low DcR3 expression (LDE) in RTECs.7 The human serum DcR3 concentration or endogenous DcR3 expression may not be high enough to cope with the modulation of the T cell response in acute T cell-mediated rejection (TCMR). We proposed a two-hit theory in human DcR3 expression in kidney allograft associated with allograft survival after kidney transplant rejection. In the acute stage of the cellular immunologic storm, DcR3 could directly induce NF-κB-mediated expression of adhesion molecules and inflammatory cytokines.9,33,35,36 Furthermore, locally expressed DcR3 in the RTECs of kidney allograft may suppress the FasL-Fas-mediated apoptosis of T cells, leading to an accumulation of activated allo-responding T cells.7,37 The second-hit could be caused by subsequent residual inflammation, indicating that HDE in the damaged tubuli could facilitate peripheral myofibroblast escaping from Fas and FasL-induced apoptosis.6

Back to Top | Article Outline

3.2. Tissue DcR3 as a biomarker for sporadic IgA nephropathy, CKD progression, and kidney allograft failure

In CKD patients, we found that the higher the expression of DcR3 in RTECs was, the greater the expression of α-smooth muscle actin (α-SMA) and fibrosis in the interstitium.6 CKD patients with HDE had a higher risk of poor composite disease outcomes, including doubling of serum creatinine and/or end-stage renal failure. The effect of this novel tissue biomarker on outcomes was more pronounced compared with conventional risk factors, such as proteinuria, diabetes mellitus, hypertension, hyperlipidemia, and eGFR.6 A potential mechanism may involve DcR3 acting as a death decoy receptor to neutralize the proapoptotic effects of FasL, LIGHT, and TL1A. Human renal myofibroblasts with inducible DcR3 by TNF-α survived FasL-induced apoptosis due to constitutive expression of Fas receptor (FasR) in human renal interstitial fibroblasts.6

The TNFRSF6B (DcR3) gene variants using high-throughput single nucleotide polymorphism (SNP) test over biopsy tissues are associated with sporadic IgA nephropathy (IgAN), with the exception of familial clustering of IgAN.38 The proposed biologic relevance of tag SNPs of the DcR3 gene, which has a positive association with IgAN, is that they serve as an antagonist to downregulate LIGHT-LTβR signaling.38 The main cause is related to an overexpression of LIGHT in Tg mice, leading to an IgAN phenotype characterized by T cell-mediated intestinal inflammation, dysregulation of immunoglobulin A production and clearance, mesangial IgA deposition, hematuria, and proteinuria.39

In RTRs, HDE in RTECs can independently predict poor graft outcome (2-fold increase in serum creatinine and/or graft failure) and significantly increase the predictability of kidney disease progression assessed by area under the ROC curve (AUC).7 Furthermore, HDE was also shown to be a biomarker of persistent insidious inflammation and further fibrosis in the repetitive allograft biopsy. We proposed previously that DcR3 is locally induced in damaged tubuli during TCMR indicating its immediate participation in the immunological response. During acute TCMR, HDE in the damaged tubuli and infiltrating leukocyte subsets have immunological effects as demonstrated by the obvious colocalization between common leukocyte antigen (CD45 surface marker) and DcR3 over infiltrating mononuclear leukocytes in the renal tubules, renal interstitium, and peritubular capillaries through immunofluorescence (IF) double staining and confocal microscopy.7 In the chronic stage, endogenous tissue DcR3 is suitable for molecular phenotypes with future graft dysfunction due to high IF staining on α-SMA in the HDE group. Near the early interstitial fibrosis and tubular atrophy area, there is also high expression of DcR3 in the residual tubules.7

Back to Top | Article Outline

3.3. Immunological phenotype of cell-mediated rejection in renal histopathology

In RTRs, the greater expression of DcR3 immunoreactivity in RTECs was correlated with acute TCMR manifesting as tubulitis and interstitial inflammation.7 The DcR3 expression was more specifically related to the severity of acute TCMR (grade 1B and grade 2A versus grade 1A) in the subgroup analysis.7

In situ hybridization study and immunohistochemical (IHC) staining were concurrently used for analyzing different severity of acute TCMR in kidney allograft. We not only found that intense DcR3 mRNA expression in RTECs infiltrated with mononuclear cells corresponded to the areas of active inflammation and severe rejection-related architecture, but also that the pattern seemed to show endogeneous DcR3 was locally produced by mononuclear cells and RTECs. There was no significant difference between HDE and LDE in severity levels of acute antibody-mediated rejection. There was a positive correlation between HDE and tubulitis. A positive correlation between HDE and interstitial mononuclear leukocyte infiltration was found, but there were no direct relationships among HDE, peritubular capillaritis, and glomerulitis.7

Back to Top | Article Outline

4. DCR3 AS A POTENTIAL THERAPEUTIC AGENT IN KIDNEY DISEASE IN VIVO

The possible utility of hydrodynamics-based gene delivery (CMV promoter-driven human DcR3 plasmid, pCMV-DcR3) and recombinant DcR3 (DcR3.Fc) can prevent the development of autoimmune crescentic glomerulonephritis in (C57BL/6 crossing DBA/2J) hybrid mice and progressive IgAN in B-cell-deficient mice by daily injection of purified IgA antiphosphorylcholine antibodies and pneumococcal C-polysaccharide (PnC).34,40 The beneficial effect might be due to modulation of T cell activation/proliferation or B cell activation.34 The other protective effect of DcR3 is against apoptosis of spleen and kidney parenchyma. Suppression of mononuclear leukocyte infiltration was evidenced by histopathology with hematoxylin and eosin stain, flow cytometry of splenic T cells, IHC staining for CD3+, F4/80+, or CD11b+, real-time PCR assay for monocyte chemoattractant protein-1 (MCP-1) and IL-6, and renal tissue NF-κB expression.40

Back to Top | Article Outline

5. DCR3 TG MICE

To understand the systemic effects of DcR3 in vivo and pathophysiologic expression in human after acute or chronic insults, Tg mice were studied using rat-insulin promoter (RIP)41 and CD68 promoter.12,14,16 IHC stain for DcR3 and insulin from Tg islet graft (overexpression of human DcR3 in islet β-cells by RIP promoter)-bearing kidneys in nonobese diabetic mice showed that DcR3 was potentially effective in prolonging islet graft survival, but did not provide permanent protection from diabetes recurrence.41 In CD68 promoter-driven DcR3 Tg (CD68-DcR3 Tg) mice, DcR3 was found to be a potent tumor-secreted factor that skews macrophage polarization toward TAM or macrophage phenotype 2.12,16 When BALB/c mice were infected with Listeria monocytogenes, DcR3 Tg mice showed up-regulation of anti-inflammatory cytokines (IL-4 and IL-10) and down-regulation of proinflammatory cytokines (IFN-γ, IL-12, and TNF-α).14 However, further study is needed to comprehensively demonstrate molecular evidence of systemic DcR3-mediated anti-inflammation in kidney disease.

Back to Top | Article Outline

6. KNOCKDOWN OF DCR3 IN HUMAN

There is evidence that knockdown of DcR3 by lentivirus-delivered short hairpin RNA inhibited ectopic adhesion of endometrium and abrogated endometriosis progression.42 Tsai et al found that DcR3 is upregulated in human ectopic endometrial cells with high serum estrogen levels, and DcR3 expression level correlates positively with adhesion molecules (ICAM-1 and homing cell adhesion molecule) via Akt-NF-κB signaling pathway.42 Knockdown of DcR3 not only suppresses endometriosis tissue growth and adhesion in an orthotropic xenograft endometriosis mouse model, but also reduces adhesion molecule expression and cell migration of the HEC1B cell line.42 There is no available data in DcR3 knockout in human renal cells, which could be more representative for the function of DcR3 in human.

In conclusion, in RTRs, DcR3 expression seemed to be associated with nonspecific expression in damaged tubuli, renal interstitum, peritubular capillaries, and infiltrating leukocyte subsets. Upregulation of DcR3 during acute inflammatory reactions induces negative-feedback to suppress inflammation. In CKD patients, DcR3 affects immunomodulation by serving as a traditional decoy receptor to antagonize FasL-Fas-mediated apoptosis of myofibroblasts. The cell-specific expression of DcR3 on RTECs seemed to be derived from TNF-α stimulation. In HD, PD, and older adults with malnourishment on HD, DcR3 functions as an effector molecule that modulates proinflammatory responses via “non-decoy” activities through NF-κB-mediated expression of ICAM-1, VCAM-1, and IL-8 by monocytes. Our sequential studies (Table) demonstrated that DcR3 may be an appropriate prognostic biomarker for patients with CKD, HD, PD, malnourishment on HD, and RTRs.

Table DcR3

Table DcR3

Back to Top | Article Outline

ACKNOWLEDGMENTS

We are deeply indebted to Taichung Veterans General Hospital, Taichung for providing the grants for this study (TCVGH-YM1050101, TCVGH-1068201B, TCVGH-YM1060103, TCVGH-1078201B, TCVGH-YM1070101, TCVGH-1088201B, TCVGH-YM1080103) and to Taipei Veterans General Hospital (V108D42-004-MY3-1 and V108C-103). This study was also supported by Taiwan’s Ministry of Science and Technology (MOST 106-2314-B-010 -039 -MY3 and106-2314-B-075A-003).

The authors sincerely appreciate the assistance of the Center for Translational Medicine of Taichung Veterans General Hospital, Taichung, Taiwan.

Back to Top | Article Outline

REFERENCES

1. Weng SC, Tarng DC. Interaction between protein-energy wasting and geriatric nutritional risk index in elderly patients on dialysis.J Chin Med Assoc201679299–300
2. Lin WW, Hsieh SL. Decoy receptor 3: a pleiotropic immunomodulator and biomarker for inflammatory diseases, autoimmune diseases and cancer.Biochem Pharmacol201181838–47
3. Hsieh SL, Lin WW. Decoy receptor 3: an endogenous immunomodulator in cancer growth and inflammatory reactions.J Biomed Sci20172439
4. Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd P, et al. Genomic amplification of a decoy receptor for fas ligand in lung and colon cancer.Nature1998396699–703
5. Chen J, Zhang L, Kim S. Quantification and detection of dcr3, a decoy receptor in TNFR family.J Immunol Methods200428563–70
6. Tseng WC, Yang WC, Yang AH, Hsieh SL, Tarng DC. Expression of TNFRSF6B in kidneys is a novel predictor for progression of chronic kidney disease.Mod Pathol201326984–94
7. Weng SC, Shu KH, Wu MJ, Wen MC, Hsieh SL, Chen NJ, et al. Expression of decoy receptor 3 in kidneys is associated with allograft survival after kidney transplant rejection.Sci Rep2015512769
8. Chang EP, Lin YS, Huang SC, Tarng DC, Huang TP. High serum dcr3 levels are associated with the occurrence of peritonitis in patients receiving chronic peritoneal dialysis.J Chin Med Assoc201275644–8
9. Hung SC, Hsu TW, Lin YP, Tarng DC. Decoy receptor 3, a novel inflammatory marker, and mortality in hemodialysis patients.Clin J Am Soc Nephrol201271257–65
10. Tsai MT, Hu FH, Lien TJ, Chen PJ, Huang TP, Tarng DC. Interaction between geriatric nutritional risk index and decoy receptor 3 predicts mortality in chronic hemodialysis patients.Am J Nephrol201440191–9
11. Sun M, Fink PJ. A new class of reverse signaling costimulators belongs to the TNF family.J Immunol20071794307–12
12. Chang YC, Chen TC, Lee CT, Yang CY, Wang HW, Wang CC, et al. Epigenetic control of MHC class II expression in tumor-associated macrophages by decoy receptor 3.Blood20081115054–63
13. Hsu TL, Chang YC, Chen SJ, Liu YJ, Chiu AW, Chio CC, et al. Modulation of dendritic cell differentiation and maturation by decoy receptor 3.J Immunol20021684846–53
14. Hsu TL, Wu YY, Chang YC, Yang CY, Lai MZ, Su WB, et al. Attenuation of th1 response in decoy receptor 3 transgenic mice.J Immunol20051755135–45
15. Chang YC, Hsu TL, Lin HH, Chio CC, Chiu AW, Chen NJ, et al. Modulation of macrophage differentiation and activation by decoy receptor 3.J Leukoc Biol200475486–94
16. Tai SK, Chang HC, Lan KL, Lee CT, Yang CY, Chen NJ, et al. Decoy receptor 3 enhances tumor progression via induction of tumor-associated macrophages.J Immunol20121882464–71
17. Chen YC, Weng SC, Liu JS, Chuang HL, Hsu CC, Tarng DC. Severe decline of estimated glomerular filtration rate associates with progressive cognitive deterioration in the elderly: a community-based cohort study.Sci Rep2017742690
18. Rifkin DE, Shlipak MG, Katz R, Fried LF, Siscovick D, Chonchol M, et al. Rapid kidney function decline and mortality risk in older adults.Arch Intern Med20081682212–8
19. Shlipak MG, Katz R, Kestenbaum B, Siscovick D, Fried L, Newman A, et al. Rapid decline of kidney function increases cardiovascular risk in the elderly.J Am Soc Nephrol2009202625–30
20. Matsushita K, Selvin E, Bash LD, Franceschini N, Astor BC, Coresh J. Change in estimated GFR associates with coronary heart disease and mortality.J Am Soc Nephrol2009202617–24
21. Al-Aly Z, Zeringue A, Fu J, Rauchman MI, McDonald JR, El-Achkar TM, et al. Rate of kidney function decline associates with mortality.J Am Soc Nephrol2010211961–9
22. Al-Aly Z, Balasubramanian S, McDonald JR, Scherrer JF, O’Hare AM. Greater variability in kidney function is associated with an increased risk of death.Kidney Int2012821208–14
23. Coresh J, Turin TC, Matsushita K, Sang Y, Ballew SH, Appel LJ, et al. Decline in estimated glomerular filtration rate and subsequent risk of end-stage renal disease and mortality.JAMA20143112518–31
24. Tseng WC, Chen YT, Ou SM, Shih CJ, Tarng DC; Taiwan Geriatric Kidney Disease (TGKD) Research GroupU-shaped association between serum uric acid levels with cardiovascular and all-cause mortality in the elderly: the role of malnourishment.J Am Heart Assoc20187e007523
25. Weng SC, Tarng DC, Chen CM, Cheng CH, Wu MJ, Chen CH, et al; CKDBHPDH investigatorsEstimated glomerular filtration rate decline is a better risk factor for outcomes of systemic disease-related nephropathy than for outcomes of primary renal diseases.Plos One20149e92881
26. Weng SC, Wu CL, Kor CT, Chiu PF, Wu MJ, Chang CC, et al. Migraine and subsequent chronic kidney disease risk: a nationwide population-based cohort study.BMJ Open20177e018483
27. Weng SC, Shu KH, Tarng DC, Wu MJ, Chen CH, Yu TM, et al. Gene polymorphisms are associated with posttransplantation diabetes mellitus among Taiwanese renal transplant recipients.Transplant Proc201244667–71
28. Shu KH, Wu MJ, Chen CH, Cheng CH, Yu TM, Chuang YW, et al. Short-term prospective study of metabolic syndrome in renal transplant recipients.Transplant Proc201446540–2
29. Yu TM, Wen MC, Wu MJ, Chen CH, Cheng CH, Li CY, et al. Impact of posttransplantation glomerulonephritis on long-term outcome of kidney transplants: single-center 20-year experience.World J Surg2012362923–30
30. Stam SP, Eisenga MF, Gomes-Neto AW, van Londen M, de Meijer VE, van Beek AP, et al. Muscle mass determined from urinary creatinine excretion rate, and muscle performance in renal transplant recipients.J Cachexia Sarcopenia Muscle201910621–9
31. Bolignano D, Lacquaniti A, Coppolino G, Campo S, Arena A, Buemi M. Neutrophil gelatinase-associated lipocalin reflects the severity of renal impairment in subjects affected by chronic kidney disease.Kidney Blood Press Res200831255–8
32. Bao H, Chen H, Zhu X, Zhang M, Yao G, Yu Y, et al. Mir-223 downregulation promotes glomerular endothelial cell activation by upregulating importin α4 and α5 in iga nephropathy.Kidney Int201485624–35
33. Yang CR, Hsieh SL, Ho FM, Lin WW. Decoy receptor 3 increases monocyte adhesion to endothelial cells via NF-kappa B-dependent up-regulation of intercellular adhesion molecule-1, VCAM-1, and IL-8 expression.J Immunol20051741647–56
34. Ka SM, Sytwu HK, Chang DM, Hsieh SL, Tsai PY, Chen A. Decoy receptor 3 ameliorates an autoimmune crescentic glomerulonephritis model in mice.J Am Soc Nephrol2007182473–85
35. Kim S, Fotiadu A, Kotoula V. Increased expression of soluble decoy receptor 3 in acutely inflamed intestinal epithelia.Clin Immunol2005115286–94
36. Wu NL, Huang DY, Hsieh SL, Hsiao CH, Lee TA, Lin WW. EGFR-driven up-regulation of decoy receptor 3 in keratinocytes contributes to the pathogenesis of psoriasis.Biochim Biophys Acta201318321538–48
37. Liang D, Hou Y, Lou X, Chen H. Decoy receptor 3 improves survival in experimental sepsis by suppressing the inflammatory response and lymphocyte apoptosis.Plos One201510e0131680
38. Liu XQ, Paterson AD, He N, St George-Hyslop P, Rauta V, Gronhagen-Riska C, et al. IL5RA and TNFRSF6B gene variants are associated with sporadic iga nephropathy.J Am Soc Nephrol2008191025–33
39. Wang J, Anders RA, Wu Q, Peng D, Cho JH, Sun Y, et al. Dysregulated LIGHT expression on T cells mediates intestinal inflammation and contributes to iga nephropathy.J Clin Invest2004113826–35
40. Ka SM, Hsieh TT, Lin SH, Yang SS, Wu CC, Sytwu HK, et al. Decoy receptor 3 inhibits renal mononuclear leukocyte infiltration and apoptosis and prevents progression of iga nephropathy in mice.Am J Physiol Renal Physiol2011301F1218–30
41. Sung HH, Juang JH, Lin YC, Kuo CH, Hung JT, Chen A, et al. Transgenic expression of decoy receptor 3 protects islets from spontaneous and chemical-induced autoimmune destruction in nonobese diabetic mice.J Exp Med20041991143–51
42. Tsai HW, Huang MT, Wang PH, Huang BS, Chen YJ, Hsieh SL. Decoy receptor 3 promotes cell adhesion and enhances endometriosis development.J Pathol2018244189–202
Keywords:

Adhesion molecules; Alloreactive T cells; Inflammatory markers; Renal tubular epithelial cells; Transgenic mice

© 2019 by Lippincott Williams & Wilkins, Inc.