Micro-computed tomography is used frequently in preclinical in vivo research. Limiting factors are radiation dose and long scan times. The purpose of the study was to compare a standard step-and-shoot to a continuous-rotation, high-speed scanning protocol.
Micro-computed tomography of a lead grid phantom and a rat femur was performed using a step-and-shoot and a continuous-rotation protocol. Detail discriminability and image quality were assessed by 3 radiologists. The signal-to-noise ratio and the modulation transfer function were calculated, and volumetric analyses of the femur were performed. The radiation dose of the scan protocols was measured using thermoluminescence dosimeters.
The 40-second continuous-rotation protocol allowed a detail discriminability comparable to the step-and-shoot protocol at significantly lower radiation doses. No marked differences in volumetric or qualitative analyses were observed.
Continuous-rotation micro-computed tomography significantly reduces scanning time and radiation dose without relevantly reducing image quality compared with a normal step-and-shoot protocol.
From the Department of Neuroradiology, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Received for publication November 22, 2010; accepted March 25, 2011.
Reprints: Marc A. Brockmann, MD, MSc, Department of Neuroradiology, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany (e-mail: email@example.com).
H.U.K. and C.T.I. contributed equally.
The micro-computed tomography (Yxlon Y. Fox) was obtained from the HBFG grant 125-648 (Land Baden-Württemberg).