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Effects of postprandial hydroxytyrosol and derivates on oxidation of LDL, cardiometabolic state and gene expression

a nutrigenomic approach for cardiovascular prevention

Perrone, Marco A.a,b,c; Gualtieri, Paolad; Gratteri, Santoe; Ali, Wahidf; Sergi, Domenicoa; Muscoli, Saverioa; Cammarano, Andread; Bernardini, Sergiob,c; Di Renzo, Laurad; Romeo, Francescoa

Journal of Cardiovascular Medicine: July 2019 - Volume 20 - Issue 7 - p 419–426
doi: 10.2459/JCM.0000000000000816
Research articles: Prevention

Background and aim Cardiovascular diseases (CVDs) are the most frequent causes of death in the world. Inflammation and oxidative damage contribute significantly to the development of atherosclerosis and CVDs. European Food Safety Authority scientific opinion has acknowledged that hydroxytyrosol (3,4-dihydroxyphenylethanol) and derivatives, contained in extra virgin olive oil (EVOO), typically used in Mediterranean diet may play a crucial role in the reduction of the inflammatory pathway and in the prevention of CVDs. The aim of the study was to determine the effect in healthy volunteers of 25 g of phenols-rich EVOO (p-EVOO).

Methods The clinical study was a randomized, controlled trial to determine the acute effect in the postprandial time of 25 g of p-EVOO. We evaluated nutritional status using anthropometric parameters, body composition, serum metabolites, oxidative stress biomarkers and gene expression of eight genes related to oxidative stress and human inflammasome pathways, lasting 2 h after p-EVOO administration. Twenty-two participants resulted as eligible for the study.

Results A significant reduction of oxidized LDL, malondialdehyde, triglycerides and visceral adiposity index was highlighted (P < 0.05). Significant upregulation of catalase, superoxide dismutase 1 and upstream transcription factor 1 were observed (P < 0.05).

Conclusion The current study shows that intake of 25 g of p-EVOO has been able to be modulated, in the postprandial time, the antioxidant profile and the expression of inflammation and oxidative stress-related genes, as superoxide dismutase 1, upstream transcription factor 1 and catalase. We also observed a significant reduction of oxidized LDL, malondialdehyde, triglycerides and visceral adiposity index. We have demonstrated that a daily intake of phenols and antioxidants can reduce the inflammatory pathway and oxidative stress and therefore the risk of atherosclerosis and CVDs. More studies on a larger population are necessary before definitive conclusions can be drawn.

Trial registration NCT01890070.

aDivision of Cardiology

bDivision of Clinical Biochemistry and Clinical Molecular Biology

cUniversity Sports Centre

dDivision of Clinical Nutrition and Nutrigenomics, University of Rome Tor Vergata, Rome

eDepartment of Surgery and Medical Science, Magna Græcia University, Catanzaro, Germaneto, Italy

fDepartment of Pathology, King George S. Medical University, Lucknow, Uttar Pradesh, India

Correspondence to Marco A. Perrone, MD, Division of Cardiology, University of Rome Tor Vergata, Rome, Italy E-mail:

Received 28 January, 2019

Revised 16 March, 2019

Accepted 14 April, 2019

© 2019 Italian Federation of Cardiology. All rights reserved.