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Does stem cell therapy induce myocardial neoangiogenesis? Histological evaluation in an ischemia/reperfusion animal model

Pennella, Soniaa,d,*; Reggiani Bonetti, Lucab,*; Migaldi, Mariob; Manenti, Antonioc; Lonardi, Robertoc; Giuliani, Enricoc; Barbieri, Albertoc; Farinetti, Albertoc; Mattioli, Anna V.a,d

Journal of Cardiovascular Medicine: April 2017 - Volume 18 - Issue 4 - p 277–282
doi: 10.2459/JCM.0000000000000357
Regenerative medicine

Background In an experimental model in the rabbit, a myocardial ischemia-reperfusion injury was obtained. Subsequently, the effects of homologous bone marrow stem cell (BMSC) administration were studied.

Methods In 21 New Zealand adult rabbits, ischemia/reperfusion damage was induced by temporary occlusion of the anterior descending coronary artery. Homologous BMSCs were isolated, cultured and re-suspended for injection at the level of the ischemic zone. We evaluated the proangiogenetic effect of intramyocardial injections of BMSC at the peri-infarcted area. Histological evaluations were made after 20 days from the surgical procedure.

Results In rabbits treated with intramyocardial BMSC administration, we demonstrated histologically capillary neoangiogenesis, without signs of tissue immunological reaction or of generation of new myocardial cells. On the contrary, only minimal neovascular supply was detected in rabbits treated with intravenous administration of BMSC. Only typical signs of ischemic myocardium injury were observed in the control group.

Conclusion These observations suggest that the effect of direct BMSC administration in ischemic myocardium could promote a capillary neoangiogenesis, which helps to prevent ischemic myocardial damage.

aDepartment of Life Science

bDepartment of Diagnostic Medicine

cDepartment of Surgery, University of Modena and RE, Modena, Italy

dIstituto Nazionale per le Ricerche Cardiovascolari

Correspondence to Anna V. Mattioli, MD, PhD, Department of life science, University of Modena and R.E., Via del pozzo, 71 41100 Modena, Italy E-mail

Received 3 June, 2015

Revised 19 November, 2015

Accepted 14 December, 2015

© 2017 Italian Federation of Cardiology. All rights reserved.