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Frozen Section Histology: A New Tool to Identify Propionibacterium acnes Infections in Revision Shoulder Arthroplasty

Commentary on an article by Matthew J. Grosso, BS, et al., “Sensitivity of Frozen Section Histology for Identifying Propionibacterium acnes Infections in Revision Shoulder Arthroplasty”

Mansat, Pierre, MD, PhD*,1

doi: 10.2106/JBJS.M.01476
Commentary and Perspective
Free
Disclosures

1University of Toulouse School of Medicine, Toulouse, France

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Commentary

Propionibacterium acnes is one of the most frequent pathogens responsible for shoulder infection, especially with failed shoulder arthroplasty1. This bacterium is a particularly slow-growing anaerobic gram-positive organism2. The clinical symptoms of infection are subtle and diagnosis is often made by presumption. Aspiration of the joint can be an option to make the diagnosis. However, sensitivity and specificity of the aspirate analyses are not optimal; because of the slow-growing nature of P. acnes, the culture must be followed for fifteen to twenty-one days and, therefore, the presence of P. acnes can be missed if the cultures have been discarded earlier3. Frozen section histology of tissues can be an option to make a more accurate diagnosis as with periprosthetic hip or knee infection4. Appropriate diagnosis can be essential to determine surgical and medical management, including extent of removal of components and cement, one or two-stage revision, and length and type of postoperative antibiotic course. Few studies have evaluated the sensitivity of frozen section analysis in the shoulder, and no studies have previously examined the performance of frozen section in P. acnes infections.

This single-center study was retrospective and comparative with control groups and analyzed all patients who underwent revision shoulder arthroplasty between 2005 and 2012. Patients with proven periprosthetic joint infection were compared with a group who had undergone aseptic revisions. Because there is no gold standard to establish the diagnosis of infection in shoulder arthroplasty, the authors have arbitrarily created a spectrum of infection categories: no evidence for infection, possible infection, probable infection, or definite infection based on the existence of negative or positive cultures and preoperative or intraoperative findings of infection. For final analysis, infected patients were subsequently divided into groups of patients who had P. acnes infections and patients who had infections from other organisms, to create three groups: (1) the non-infection group, (2) the P. acnes infection group, and (3) the other infection group. Intraoperative frozen section histology specimens were collected for forty-five patients for reanalysis. All tissue samples that were evaluated were deep periprosthetic specimens, obtained from along the interfaces of the glenoid or humeral components or adjacent to the implants. Anaerobic tissue and fluid cultures were incubated systematically for fourteen days. For all specimens, the total number of polymorphonuclear leukocytes in the five high-power fields (400×) with the highest concentration of polymorphonuclear leukocytes was determined4.

The results of this study have shown the low sensitivity of the erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) to diagnose P. acnes infection; the P. acnes infection group had a sensitivity of 61% for ESR and 33% for CRP. The specificity of ESR and CRP was 85% for both the P. acnes infection group and the other infection group. The sensitivity of frozen section histology for P. acnes infection was 50% and the specificity was 100% on the basis of the current institutional guidelines. Using the American Academy of Orthopaedic Surgeons (AAOS) guidelines of ten polymorphonuclear leukocytes per high-power field for five or more fields, sensitivity was only 39%4. However, there was no false positive using any of the thresholds, yielding a specificity of 100%. Using a cutoff from seven to ten polymorphonuclear leukocytes, the sensitivity of frozen section histology for P. acnes infection improved to 72%, while maintaining 100% specificity.

The results of this study showed that the guidelines recommended by the AAOS Practice Guidelines Committee for Diagnosing Periprosthetic Joint Infections of the Hip and Knee had high specificity (no false positives) but relatively low sensitivity when applied to revision shoulder arthroplasty to diagnose P. acnes infection4. A new threshold of a total of ten or more polymorphonuclear leukocytes in five high-power fields may help increase the sensitivity of frozen sections to diagnose the commonly indolent periprosthetic infections of the shoulder with minimal impact on specificity.

When a revision of a failed shoulder arthroplasty has been planned, the presence of a positive frozen section may lead the treating surgeon to maintain antibiotic therapy postoperatively for fifteen to twenty-one days to avoid a delay in treatment and to perform a two-stage exchange instead of a one-stage revision5,6. Furthermore, with the high specificity of the frozen section histology, the risk of excessive treatment remains low. The presence of acute inflammation in periprosthetic tissue can support the diagnosis of infection in cases with one unexpected positive culture. If the frozen section histology does not show acute inflammation, then it further supports the hypothesis that a single unexpected positive culture after revision may not require further treatment.

The weakness of this study is related to its retrospective characteristics. Only patients with failed shoulder arthroplasty have been included. The results may not be applicable to infection after other shoulder procedures (such as cuff repair or procedures for the management of proximal humeral fracture, periprosthetic fracture, or inflammatory conditions). Only patients with a high probability of having true infections were included to reduce the probability that the lower sensitivity of frozen section in the P. acnes infection group was a result of increased false-positive infections (i.e., contaminant cultures). Therefore, using an arbitrary classification, only patients who were classified as having a definite infection or a probable infection were included. A prospective study using frozen section histology for all revision shoulder cases must be done to confirm these results.

We can gain from this study the knowledge that frozen section histology can be a helpful tool for identifying P. acnes infection intraoperatively around failed shoulder arthroplasty. Sensitivity was improved by utilizing a new histological threshold value of ten polymorphonuclear leukocytes per five high-power fields, while maintaining 100% specificity.

*The author received no payments or services, either directly or indirectly (i.e., via his institution), from a third party in support of any aspect of this work. Neither the author nor his institution has had any financial relationship, in the thirty-six months prior to submission of this work, with any entity in the biomedical arena that could be perceived to influence or have the potential to influence what is written in this work. Also, the author has not had any other relationships, or engaged in any other activities, that could be perceived to influence or have the potential to influence what is written in this work. The complete Disclosures of Potential Conflicts of Interest submitted by authors are always provided with the online version of the article.

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References

1. Sperling JW, Kozak TK, Hanssen AD, Cofield RH . Infection after shoulder arthroplasty. Clin Orthop Relat Res. 2001 Jan;(382):206-16.
2. Brook I, Frazier EH . Infections caused by Propionibacterium species. Rev Infect Dis. 1991 Sep-Oct;13(5):819-22.
3. Dodson CC, Craig EV, Cordasco FA, Dines DM, Dines JS, Dicarlo E, Brause BD, Warren RF . Propionibacterium acnes infection after shoulder arthroplasty: a diagnostic challenge. J Shoulder Elbow Surg. 2010 Mar;19(2):303-7. Epub 2009 Nov 1.
4. Della Valle C, Parvizi J, Bauer TW, Dicesare PE, Evans RP, Segreti J, Spangehl M, Watters WC 3rd, Keith M, Turkelson CM, Wies JL, Sluka P, Hitchcock K ; American Academy of Orthopaedic Surgeons. Diagnosis of periprosthetic joint infections of the hip and knee. J Am Acad Orthop Surg. 2010 Dec;18(12):760-70.
5. Kelly JD 2nd, Hobgood ER . Positive culture rate in revision shoulder arthroplasty. Clin Orthop Relat Res. 2009 Sep;467(9):2343-8. Epub 2009 May 12.
6. Topolski MS, Chin PYK, Sperling JW, Cofield RH . Revision shoulder arthroplasty with positive intraoperative cultures: the value of preoperative studies and intraoperative histology. J Shoulder Elbow Surg. 2006 Jul-Aug;15(4):402-6.
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