A patient presenting with an infected diabetic foot ulcer and Staphylococcus aureus chronic osteomyelitis was studied to validate the clinical importance of bacterial colonization of osteocytic-canalicular networks, as we recently reported in a mouse model. We utilized transmission electron microscopy to describe the deformation of S. aureus, from round cocci to rod-shaped bacteria, in the submicron osteocytic-canalicular networks of amputated bone tissue.
To our knowledge, this is the first evidence of S. aureus deformation and invasion of the osteocytic-canalicular system in human bone, which supports a new mechanism of persistence in the pathogenesis of chronic osteomyelitis.
1Center for Musculoskeletal Research (K.L.d.M.B., A.M., E.M.S., and I.O.) and Departments of Pathology and Laboratory Medicine (K.L.d.M.B. and E.M.S.) and Orthopaedics (K.L.d.M.B., A.M., E.M.S., and I.O.), University of Rochester School of Medicine and Dentistry, Rochester, New York
aE-mail address for I. Oh: firstname.lastname@example.org
bE-mail address for K.L. de Mesy Bentley: email@example.com