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Erratum

The mechanisms of colorectal cancer cell mesenchymal–epithelial transition induced by hepatocyte exosome-derived miR-203a-3p

Erratum

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doi: 10.1097/JBR.0000000000000048
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In the paper titled “The mechanisms of colorectal cancer cell mesenchymal–epithelial transition induced by hepatocyte exosome-derived miR-203a-3p”, published on pages 62–72, Issue 2, Volume 1 of Journal of Bio-X Research,[1]Figures 2G and 5F (including the legend of Figure 5) appear incorrectly in the published article. Please see the correct Figures 2G and 5F and the legend of Figure 5 here.

Figure 2
Figure 2:
Co-culture with hepatocytes resulted in the re-expression of E-cadherin in colorectal cancer (CRC) cells. (A) PRL3 expression in stably transfected cell lines (LoVo-P and LoVo-C) was analyzed by western blotting. GAPDH served as the loading control. (B, C) LoVo-P cells were co-cultured with human hepatic LO2 cells for 0–3 days. The protein levels of E-cad (B) and Snail (C) were analyzed by western blotting. GAPDH served as the loading control. ** P < 0.01. (D and E) Immunoreactivity of E-cadherin (D) and Snail (E) detected by immunofluorescence staining. The nuclei were visualized with DAPI staining (blue). E-cadherin and Snail (red), PRL3 (green), and DAPI (blue) were merged. Original magnification, 400×. (F) Src and p-EGFR protein levels in LoVo-P cells co-cultured with LO2 cells were evaluated by western blotting. (G) Representative IHC images of migrated and invaded cells (left). Cells were co-cultured with human hepatic LO2 cells for 0 to 3 days and then harvested to perform invasion and migration assays for an additional 24 hours. Scale bar = 50 mm. Number of migrated and invaded cells (right). The data represent the average of three independent experiments. ** P < 0.01.
Figure 5
Figure 5:
Expression of miR-203a-3p and E-cadherin in vivo. BALB/c-nu/nu female mice aged 6 to 8 weeks were divided randomly into 2 groups and intrasplenically injected with LoVo-P or LoVo-C. (A) Images of tumors in spleen and liver. (B and C) Liver metastasis score and weight of mice. qPCR (D) and western blot assays (E) examining the expression of miR-203a-3p, Src, p-EGFR, p-PKC, and E-cadherin in the spleen and liver metastases. (F) IHC for p-EGFR, p-PKC, and E-cadherin in the spleen and liver metastases. (G) Expression of miR-203a-3p/E-cadherin (E-cad)/Snail in the primary site and liver metastases. (H) Correlation between miR-203a-3p/E-cadherin (E-cad) expression in the primary site and liver metastases. * P < 0.05, ** P < 0.01.

REFERENCE

[1]. Xu H, Huang Y, Lan Q, et al The mechanisms of colorectal cancer cell mesenchymal–epithelial transition induced by hepatocyte exosome-derived miR-203a-3p. J Bio-X Res 2018;1 2:62–72.
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