REVIEW ARTICLE: PDF OnlyInterrogating genome function using CRISPR toolsRen, Xingjiea; Takagi, Maya Asamia; Shen, Yina,b,*Author Information aInstitute for Human Genetics bDepartment of Neurology, University of California, San Francisco, San Francisco, CA, USA Corresponding author: Yin Shen, (1) Institute for Human Genetics, and (2) Department of Neurology, University of California, San Francisco, San Francisco, CA, USA. E-mail: email@example.com. Received February 25, 2020 Accepted April 1, 2020 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 Journal of Bio-X Research: August 05, 2020 - Volume Latest Articles - Issue - doi: 10.1097/JBR.0000000000000066 Open PAP Metrics Abstract Genome editing serves as a powerful approach to interrogate the functions of both coding and noncoding sequences. In particular, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associated protein 9 (Cas9) system-based editing tools have revolutionized the way we study genome function in mammalian cells, and are being widely used for interrogating critical genes and DNA elements essential for many biological processes. Here, we review CRISPR/Cas9-based genetic tools with an emphasis on CRISPR-mediated high throughput genetic screens in the mammalian genome. Copyright © 2020 The Chinese Medical Association. Published by Wolters Kluwer Health, Inc.