REVIEW ARTICLE: PDF OnlyA review of single-nucleotide polymorphism detection methods and their application in studies of Staphylococcus aureusJian, Ying; Li, Min*Author Information Department of Laboratory Medicine, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, China Corresponding author: Min Li, Department of Laboratory Medicine, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai 200127, China. E-mail: firstname.lastname@example.org. Received April 30, 2020 Accepted June 3, 2020 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 Journal of Bio-X Research: August 05, 2020 - Volume Latest Articles - Issue - doi: 10.1097/JBR.0000000000000071 Open PAP Metrics Abstract Single-nucleotide polymorphisms (SNPs) are the third generation of genetic markers, having been refined from the first generation of restriction fragment length polymorphisms and the second generation of microsatellite polymorphisms. SNPs represent a focal point of current studies of Staphylococcus aureus. Recent methodologies for detecting SNPs have typically included DNA genome sequencing methods and PCR-based detection methods. Alternative methods, such as mass spectrometry, denaturing high-performance liquid chromatography, SNaPshot, and SNP array have also been employed for SNP analysis. SNP analysis can be applied to investigate epidemiological outbreaks and transmission of Staphylococcus aureus infections, the transmission and evolution of antimicrobial resistance genes in Staphylococcus aureus isolates, interactions of Staphylococcus aureus with other bacteria, and the links between Staphylococcus aureus in humans and livestock. Copyright © 2020 The Chinese Medical Association. Published by Wolters Kluwer Health, Inc.