The Effect of Fructose on Renal Biology and Disease : Journal of the American Society of Nephrology

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Up Front Matters: Science in Renal Medicine

The Effect of Fructose on Renal Biology and Disease

Johnson, Richard J.*; Sanchez-Lozada, L. Gabriela; Nakagawa, Takahiko*

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Journal of the American Society of Nephrology 21(12):p 2036-2039, December 2010. | DOI: 10.1681/ASN.2010050506
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Abstract

Fructose is a simple sugar, a monosaccharide that is present primarily in added dietary sugars, honey, and fruit. The primary sources of fructose in the American diet are from sucrose, a disaccharide containing 50% fructose and 50% glucose bonded together, and high fructose corn syrup (HFCS), which is a mixture of free fructose and free glucose, usually in a 55/45 proportion. Intake of fructose has increased dramatically over the last century, has accelerated since the introduction of HFCS in the 1970s,1 and likely contributes to the epidemic of human obesity.2 Although the mean intake of fructose is currently about 74 g/day,3 many individuals, especially adolescents, African American adults, and Hispanic adults, are ingesting as much as 30% of their diet as added sugars. This represents >25 times what was ingested in the United Kingdom in 1700.

FRUCTOSE METABOLISM

Fructose is distinct from glucose in its initial metabolism. The Glut5 transporter in the intestine absorbs fructose with 60 to 70% being taken up by Glut2 and possibly other transporters in the liver and 30 to 40% by the kidney, adipose tissue, and other organs. Although fructose can be metabolized by hexokinase, most fructose is metabolized by fructokinase (ketohexokinase), which phosphorylates fructose to fructose 1-phosphate (Figure 1). Unlike glucose, whose phosphorylation is tightly regulated so that ATP levels are never depleted, the phosphorylation of fructose results in a decrease in intracellular phosphate and ATP depletion, resulting in transient inhibition of protein synthesis. Adenosine monophosphate is generated and broken down by adenosine monophosphate deaminase, resulting in the generation of inosine monophosphate and eventually uric acid. Uric acid rises in the cell and may transiently increase by 1 to 2 mg/dl in the circulation. Fructose-1-phosphate is then metabolized by aldolase B and other enzymes, eventually generating the acyl glycerol, diacylglycerol, with the formation of glycogen and triglycerides.4

F1-8
Figure 1:
A pathway by which fructose is metabolized into uric acid. KHK, ketohexokinase.

FRUCTOSE AND HYPERTENSION

Recent studies suggest that fructose is a mediator of hypertension, and the mechanism may involve the ability of fructose to raise uric acid. First, epidemiologic studies link fructose intake to the development of hypertension. In the NHANES (1999–2004) survey, a tight association of sugar-sweetened drinks with uric acid levels and hypertension was observed in adolescents.5 Similarly, in the NHANES (2003–2006) survey, fructose intake from added sugars correlated with the presence of elevated BP.3 Second, the ingestion of fructose-rich drinks raises BP. For example, the acute ingestion of 60 g of fructose (comparable to two 12-oz soft drinks), but not glucose, will increase BP in young healthy adults. We also reported that the administration of 200 g of fructose per day for 2 weeks raises both clinic and 24-hour ambulatory BP in healthy adult men in association with a significant rise in fasting levels of serum uric acid.6 In this study, one half of the patients were randomized to receive allopurinol, and in this group, the serum uric acid was lowered and the rise in BP prevented.6

More recently, two groups have administered low-fructose diets. In a study in Mexico City, Madero et al. found that a low-fructose diet reduces serum uric acid, with a significant decrease in BP in obese adults (M. Madero et al., submitted). In another study of 810 adults involving behavior intervention, a reduction in one sugar-sweetened beverage per day translated into a reduction of 1.8 mmHg systolic and 1.1 mmHg diastolic blood pressure at 18 months.7 Interestingly, in the latter study, the effect was independent of serum uric acid, but because it is the intracellular uric acid levels that mediate the vascular effects, it may still be dependent on a uric acid–driven pathway.

In contrast to the above studies, Forman et al.8 was unable to find an association of fructose intake with hypertension in studies based on nurses and health professionals. However, in these groups, a large amount of fructose was from natural fruits, which are known to be high in antioxidants and flavenols that can block the pro-hypertensive effects of fructose in animals; in addition, ascorbate also lowers uric acid by stimulating renal excretion. Also challenging the fructose hypothesis has been the observation that fructose does not increase BP very effectively in laboratory rats, except during active ingestion. This may be because rats carry the enzyme uricase and, as such, show a blunted uric acid response to fructose.

FRUCTOSE AND METABOLIC SYNDROME

Excessive intake of fructose, primarily in the form of added dietary sugars, has also been linked epidemiologically with the development of obesity, diabetes, and nonalcoholic fatty liver disease. More importantly, the administration of fructose to humans induces all of the features of metabolic syndrome. In a study by Stanhope et al.,9 a diet containing 25% fructose was administered for 6 weeks to overweight adults; control subjects received 25% glucose. Subjects on the high-fructose diet developed insulin resistance, visceral obesity (measured by computed tomography scan), and postprandial dyslipidemia. In the Menorca6 study, the administration of 200 g fructose per day resulted in a 25% increase in metabolic syndrome, with a significant increase in fasting triglycerides, a fall in HDL cholesterol, a rise in systolic and diastolic BP, and a worsening of insulin resistance measured using the homeostasis model assessment index.

Studies in animals have also found that fructose induces all features of the metabolic syndrome, especially when administered at high doses.10 However, metabolic syndrome can be induced with concentrations of fructose as low as 20% if it is administered with glucose, either as sucrose or HFCS, likely because the presence of glucose accelerates fructose absorption.11

Furthermore, a striking finding is that the effects of fructose to induce metabolic syndrome are independent of energy intake and can even be induced in the setting of caloric restriction.12 For example, we found that a 40% sucrose diet (which contains 20% fructose) given to rats at approximately 90% of regular intake for 4 months induces hypertriglyceridemia, insulin resistance, fatty liver, and β islet dysfunction with the development of frank diabetes; in contrast, control rats administered starch show much less metabolic disturbance (Roncal et al., submitted).

The mechanism for the development of metabolic syndrome may relate to the influx of free fatty acids with intracellular lipid accumulation in nonadipocyte tissues such as the liver.13 Fructose is also known to induce oxidative stress and mitochondrial dysfunction, resulting in a stimulation of peroxisome proliferator-activated receptor gamma coactivator 1-α that drives insulin resistance.14 Finally, there is some evidence that the rise in intracellular uric acid may have a role in mediating insulin resistance, perhaps by direct effects on the adipocyte15 or possibly by impairing endothelial nitric oxide release in response to insulin, which subsequently blocks blood flow and hence delivery of glucose to peripheral tissues such as skeletal muscle.16

FRUCTOSE AND RENAL DISEASE

The possibility that fructose may have a role in chronic kidney disease is increasingly likely, given its potential role in driving hypertension and diabetes. Indeed, the observation that metabolic syndrome is a significant risk factor for chronic kidney disease17 raises the possibility that fructose may have a causal role. Nevertheless, there is currently only one human study that examines the relationship of intake of sugar-containing beverages with renal disease. Specifically, the NHANES (1999–2004) found that intake of two or more sugar-containing beverages was associated with an increased risk of having albuminuria.18

However, experimental studies support fructose intake as a mechanism for kidney injury. For example, we found the administration of fructose (60% diet) to rats induces renal hypertrophy with tubular cell proliferation and low-grade tubulointerstitial injury, generating chemotactic factors such as monocyte chemoattractant protein-1 by tubular cells and intercellular adhesion molecule-1 in renal microvascular endothelial cells.1922 We also found the administration of fructose (60% diet) exacerbates proteinuria, worsens renal function, and accelerates glomerulosclerosis in the remnant kidney model; importantly, these abnormalities are not observed in rats fed an equivalent glucose-based diet.22 Finally, micropuncture studies documented that fructose intake results in glomerular hypertension and reduced renal blood flow in association with the development of preglomerular vascular disease.23 These findings are similar to what is observed in hyperuricemic rats, and consistent with this hypothesis, we found that lowering uric acid blocks these hemodynamic effects.23

CONCLUSIONS

There is increasing evidence that excessive intake of fructose may have a myriad of unfortunate health effects, including raising BP, inducing the metabolic syndrome, causing fatty liver, and possibly causing or accelerating renal disease (Figure 2). Given that many physicians recommend low-protein diets for subjects with chronic kidney disease, which could translate into a high-carbohydrate, high-fructose diet, we recommend that protein restriction should also include restricting added sugars containing fructose. We also believe that more clinical studies are needed to determine the effect of dietary fructose restriction or of lowering uric acid in subjects with chronic kidney disease.

F2-8
Figure 2:
Effects of fructose in the development of hypertension, fatty liver, diabetes, and renal disease.

DISCLOSURES

Drs. Johnson and Nakagawa are listed as inventors on several patent applications related to lowering uric acid as a means to prevent or treat metabolic syndrome or hypertension. Dr. Johnson also has a book in the lay press, The Sugar Fix (Rodale, and Simon and Schuster).

Support for this paper was provided by National Institutes of Health Grant HL-68607 and startup funds from the University of Colorado.

Published online ahead of print. Publication date available at www.jasn.org.

REFERENCES

1. Neilson EG: The fructose nation. J Am Soc Nephrol 18: 2619–2621, 2007
2. Bray GA, Nielsen SJ, Popkin BM: Consumption of high-fructose corn syrup in beverages may play a role in the epidemic of obesity. Am J Clin Nutr 79: 537–543, 2004
3. Jalal DI, Smits G, Johnson RJ, Chonchol M: Increased fructose associates with elevated blood pressure. J Am Soc Nephrol 21: 1543–1549, 2010
4. Van den Berghe G: Fructose: Metabolism and short-term effects on carbohydrate and purine metabolic pathways. Prog Biochem Pharmacol 21: 1–32, 1986
5. Nguyen S, Choi HK, Lustig RH, Hsu CY: Sugar-sweetened beverages, serum uric acid, and blood pressure in adolescents. J Pediatr 154: 807–813, 2009
6. Perez-Pozo SE, Schold J, Nakagawa T, Sanchez-Lozada LG, Johnson RJ, Lillo JL: Excessive fructose intake induces the features of metabolic syndrome in healthy adult men: Role of uric acid in the hypertensive response. Int J Obes (Lond) 34: 454–461, 2010
7. Chen L, Caballero B, Mitchell DC, Loria C, Lin PH, Champagne CM, Elmer PJ, Ard JD, Batch BC, Anderson CA, Appel LJ: Reducing consumption of sugar-sweetened beverages is associated with reduced blood pressure: a prospective study among United States adults. Circulation 121: 2398–2406, 2010
8. Forman JP, Choi H, Curhan GC: Fructose and vitamin C intake do not influence risk for developing hypertension. J Am Soc Nephrol 20: 863–871, 2009
9. Stanhope KL, Schwarz JM, Keim NL, Griffen SC, Bremer AA, Graham JL, Hatcher B, Cox CL, Dyachenko A, Zhang W, McGahan JP, Seibert A, Krauss RM, Chiu S, Schaefer EJ, Ai M, Otokozawa S, Nakajima K, Nakano T, Beysen C, Hellerstein MK, Berglund L, Havel PJ: Consuming fructose-sweetened, not glucose-sweetened, beverages increases visceral adiposity and lipids and decreases insulin sensitivity in overweight/obese humans. J Clin Invest 119: 1322–1334, 2009
10. Johnson RJ, Perez-Pozo SE, Sautin YY, Manitius J, Sanchez-Lozada LG, Feig DI, Shafiu M, Segal M, Glassock RJ, Shimada M, Roncal C, Nakagawa T: Hypothesis: Could excessive fructose intake and uric acid cause type 2 diabetes? Endocr Rev 30: 96–116, 2009
11. Fujisawa T, Riby J, Kretchmer N: Intestinal absorption of fructose in the rat. Gastroenterology 101: 360–367, 1991
12. Roncal CA, Reungjui S, Sanchez-Lozada LG, Mu W, Sautin YY, Nakagawa T, Johnson RJ: Combination of captopril and allopurinol retards fructose-induced metabolic syndrome. Am J Nephrol 30: 399–404, 2009
13. Stanhope KL, Havel PJ: Fructose consumption: Potential mechanisms for its effects to increase visceral adiposity and induce dyslipidemia and insulin resistance. Curr Opin Lipidol 19: 16–24, 2008
14. Nagai Y, Yonemitsu S, Erion DM, Iwasaki T, Stark R, Weismann D, Dong J, Zhang D, Jurczak MJ, Loffler MG, Cresswell J, Yu XX, Murray SF, Bhanot S, Monia BP, Bogan JS, Samuel V, Shulman GI: The role of peroxisome proliferator-activated receptor gamma coactivator-1 beta in the pathogenesis of fructose-induced insulin resistance. Cell Metab 9: 252–264, 2009
15. Sautin YY, Nakagawa T, Zharikov S, Johnson RJ: Adverse effects of the classic antioxidant uric acid in adipocytes: NADPH oxidase-mediated oxidative/nitrosative stress. Am J Physiol Cell Physiol 293: C584–C596, 2007
16. Nakagawa T, Hu H, Zharikov S, Tuttle KR, Short RA, Glushakova O, Ouyang X, Feig DI, Block ER, Herrera-Acosta J, Patel JM, Johnson RJ: A causal role for uric acid in fructose-induced metabolic syndrome. Am J Physiol Renal Physiol 290: F625–F631, 2006
17. Chen J, Muntner P, Hamm LL, Jones DW, Batuman V, Fonseca V, Whelton PK, He J: The metabolic syndrome and chronic kidney disease in U.S. adults. Ann Intern Med 140: 167–174, 2004
18. Shoham DA, Durazo-Arvizu R, Kramer H, Luke A, Vupputuri S, Kshirsagar A, Cooper RS: Sugary soda consumption and albuminuria: results from the National Health and Nutrition Examination Survey, 1999–2004. PLoS ONE 3: e3431, 2008
19. Nakayama T, Kosugi T, Connor T, Gersch M, Sanchez-Lozada LG, Roncal C, Perez-Pozo SE, Johnson RJ, Nakagawa T: Dietary fructose causes tubulointerstitial injury in the normal rat kidney. Am J Physiol Renal Physiol 298: F712–F720, 2010
20. Cirillo P, Gersch MS, Mu W, Scherer PM, Kim KM, Gesualdo L, Henderson GN, Johnson RJ, Sautin YY: Ketohexokinase-dependent metabolism of fructose induces proinflammatory mediators in proximal tubular cells. J Am Soc Nephrol 20: 545–553, 2009
21. Glushakova O, Kosugi T, Roncal C, Mu W, Heinig M, Cirillo P, Sanchez-Lozada LG, Johnson RJ, Nakagawa T: Fructose induces the inflammatory molecule ICAM-1 in endothelial cells. J Am Soc Nephrol 19: 1712–1720, 2008
22. Gersch MS, Mu W, Cirillo P, Reungjui S, Zhang L, Roncal C, Sautin YY, Johnson RJ, Nakagawa T: Fructose, but not dextrose, accelerates the progression of chronic kidney disease. Am J Physiol Renal Physiol 293: F1256–F1261, 2007
23. Sanchez-Lozada LG, Tapia E, Bautista-Garcia P, Soto V, Avila-Casado C, Vega-Campos IP, Nakagawa T, Zhao L, Franco M, Johnson RJ: Effects of febuxostat on metabolic and renal alterations in rats with fructose-induced metabolic syndrome. Am J Physiol Renal Physiol 294: F710–F718, 2008
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