Fabry disease (FD) is an X-linked rare progressive multisystemic disorder resulting from lysosomal enzyme α -galactosidase A (GLA) deficiency. Fabry-specific manifestations, such as early stroke, malignant arrhythmia, myocardial infarction, and progressive renal and cardiac failure, result from the differential systemic cellular accumulation of globotriaosylceramide (Gb3).1 α (Replagal, Shire) and agalsidase-β (Fabrazyme, Genzyme), results in subcellular Gb3 clearance, leading to stabilization or at least slowing of disease progression.2 – 7
The shortage in agalsidase-β supply (from June 2009 to January 2012) resulted in a change of treatment regimen in many patients. Patients who had formerly received agalsidase-β in a standard dose (1.0 mg/kg body wt every other week [e.o.w.]) were dose-reduced (0.3–0.5 mg/kg body wt e.o.w.) or switched to agalsidase-α (0.2 mg/kg body wt e.o.w.). Very limited clinical data on the basis of small potentially statistically underpowered8 9 – 12 β dose had a stable disease course, but dose reduction was associated with worsening of renal function and symptoms.13 α was safe, but renal function declined and Fabry-related symptoms increased in some patients.13
Because the 1-year follow-up period was relatively short, a long-term follow-up study of patients who were dose reduced and/or switched is warranted.
In this study we assessed clinical stability and safety during agalsidase-β standard dose, ERT dose reduction (agalsidase-β , 0.3 or 0.5 mg/kg e.o.w.), and subsequent switch or a direct switch to agalsidase-α (0.2 mg/kg) after 2 years of observation, with a special focus on clinical events, renal end organ damage, disease-related clinical symptoms, and disease severity.
Results
Patients
Figure 1 represents an overview of this retrospective observational study. A comprehensive diagnostic workup was performed as previously reported.13 Tables 1 –3 . In total, 89 patients with FD were included. The mean age was 45.5±12.5 years, and 37.1% were women. Patients received agalsidase-β , 1.0 mg/kg body wt e.o.w. for 44.2±26.3 months at baseline. Because of the ongoing supply shortage of agalsidase-β , patients receiving 0.5 mg/kg e.o.w. were switched to agalsidase-α (0.2 mg/kg e.o.w.) after 12 months of observation (Figure 1 ). Because of the observational design (real-world design), patients were not randomized. The long-term treatment strategy was decided by the interdisciplinary physicians of the Fabry centers. This strategy resulted in quite inhomogeneous treatment groups, with more severely affected patients with FD in the regular-dose group and suspected milder affected patients within the dose-reduction-switch and switch groups. In detail, compared with the switch-group and the dose-reduction-switch group, the regular-dose group comprised more male patients (each P <0.05) (Table 1 ), resulting in a lower residual enzymatic GLA activity (each P <0.05) (Table 1 ). In terms of FD-typical symptoms, patients in the regular-dose group more often showed edema compared with the dose-reduction-switch group (P <0.05) (Table 1 ). In addition, patients in the regular-dose group and dose-reduction-switch groups suffered more often from pain attacks compared with those in the switch group (each P <0.05) (Table 1 ).
Figure 1: General overview of the study population. All patients were at least 12 months under a stable dose of agalsidase-β (1.0 mg/kg e.o.w.). After the baseline visit, the treatment strategy for the following years was chosen by a FD specialist physician team and the patients. Because of ongoing supply shortage, patients under agalsidase-β reduction (0.5 mg/kg e.o.w.) were switched after 12 months to agalsidase-α (0.2 mg/kg e.o.w.). *Patients were at least 12 months under a regular-dose of agalsidase-β .
Table 1: Baseline characteristics of the patient groups
At baseline, all three groups did not significantly differ in eGFR on the basis of different formulae (Table 2 ). Cardiac measures revealed only a slightly increased left ventricular diastolic diameter in the regular-dose group in comparison with the switch group (P <0.05) (Table 3 ). Neurologic measures revealed no differences in baseline parameters between the groups (Table 3 ).
Table 2: Baseline renal measures of the patient groups
Table 3: Baseline cardiac and neurologic measures of the patient groups
Outcome for Clinical Events
The outcome for serious clinical events, such as stroke/transient ischemic attack, dialysis, renal transplantation, or a pacemaker/implantable cardioverter defibrillator, did not significantly differ between baseline and 2-year follow-up in any of the three groups (Table 4 ).
Table 4: Development of major events between baseline and 2-year follow-up
Outcome for Change in Organ Function and Structure
Because we recently reported a significant impairment of renal function after 12 months of agalsidase-β dose-reduction or switch to agalsidase-α ,13 1 ) creatinine-based (eGFRcreat ) according to Levey and colleagues,14 2 ) cystatin C-based (eGFRcys ), and (3 ) creatinine/cystatin C-based (eGFRcreat-cys ), both according to Inker and colleagues,15 16 i.e., annual changes) have been adjusted for baseline values (Table 5 ). eGFR values of patients within the regular-dose group remained stable between baseline and 2-year follow-up, independent of the used eGFR formula for calculation (Figure 2 , Table 5 ). Within the dose-reduction-switch group, eGFRcreat values decreased by 5.00% per year or –3.74 ml/min per 1.73 m2 per y (adjusted), respectively (Figure 2 , Table 5 ). Similar results were obtained by cystatin C–based and creatinine/cystatin C–based eGFR calculations in that both calculations revealed significant decreases between baseline and 2-year follow-up (Figure 2 , Table 5 ). Within the switch group, the annual percentage change for eGFRcreat remained stable, but the multivariate regression model revealed a slight decline of 2.45 ml/min per 1.73 m2 per y after adjustment (Table 5 ). In addition, cystatin C–based and creatinine/cystatin C–based eGFR values decreased significantly over time within this group (Table 5 ).
Table 5: eGFRs determined by creatinine-, cystatin C- and creatinine/cystatin C–based CKD-EPI formulae and changes per year
Figure 2: Changes in renal function quantified by eGFR over time. eGFR was quantified using the CKD-EPI equation on the basis of (A) creatinine (eGFRcreat ), (B) cystatin C (eGFRcys ), and (C) the combination of both levels (eGFRcreat-cys ). Patients under dialysis, with renal transplantation or hyperfiltration (>120 ml/min per 1.73 m2 on the basis of eGFRcreat ) have been excluded. Dark green color represents the regular-dose group, bright green color represents the dose-reduction-switch group, and red represents the switch group. Values are given in mean±SD. **P <0.01.
The analyses of raw serum creatinine and cystatin C concentrations over time in all three groups showed that creatinine values remained stable within the regular-dose (P =0.39) and switch groups (P =0.43) but increased significantly within the dose-reduction-switch group (P <0.01) (Supplemental Figure 1). Raw cystatin C values remained also stable over time within the regular-dose group (P =0.83), but they increased within the dose-reduction-switch and the switch groups (both P <0.01) (Supplemental Figure 1).
ACR values did not differ between baseline and 2-year follow-up in any of the three groups (Figure 3 ). In detail, median ACRs differed not significantly between the three groups at baseline (regular-dose group: 81 [range, 0–741] mg/g; dose-reduction-switch group: 162 [range, 0–1292] mg/g; switch group: 57 [range, 0–1426] mg/g; P =0.49). After 2 years, all three groups revealed stable ACRs (Figure 3 ). Additionally, ACRs at 2-year follow-up did not differ between the groups (regular-dose group: 188 [range, 8–2420] mg/g; dose-reduction-switch group: 103 [range, 5–552] mg/g; switch group: 76 [range, 0–1457] mg/g; P =0.71).
Figure 3: Changes in ACR over time. Patients under dialysis, with renal transplantation, or hyperfiltration (>120 ml/min per 1.73 m2 on the basis of eGFRcreat ) have been excluded. Dark green color represents the regular-dose group, bright green color represents the dose-reduction-switch group, and red represents the switch group. Values are given as medians (95% confidence interval).
Outcome for Changes in FD-Related Symptoms
Changes in FD-related symptoms are shown in Table 6 . Frequencies of pain attacks, permanent pain, and diarrhea remained stable in all three groups between baseline and 2-year follow-up (Table 6 ). However, frequencies of gastrointestinal pain increased significantly in the dose-reduction-switch group (relative risk: 1.87; 95% confidence interval, 1.18 to 2.95) and the switch group (relative risk: 1.73; 95% confidence interval, 1.10 to 2.71) after 2 years of observation (Table 6 ). As a marker of general disease progression and severity, Mainz Severity Score Index (MSSI) scores were assessed (Table 7 ). Because the MSSI scores might be influenced by nonchangeable aspects, only values within the groups have been analyzed between baseline and 2-year follow-up. Although patients in the regular-dose group remained stable, patients in the dose-reduction-switch group and switch group showed increases of MSSI scores after 2 years (P <0.05 and P <0.001, respectively) (Table 7 ).
Table 6: Development of symptoms between baseline and 2-year follow-up
Table 7: Differences in MSSI score, LVSd, and ejection fraction between baseline and 2-year follow-up
In terms of cardiac involvement (i.e., left ventricular septum in diastole and ejection fraction), no differences were detected within the three groups during observation (Table 7 ).
Additional Medication
Information on additional medication (diuretics, renin-angiotensin-aldosterone system blockers, and analgesics) is presented in Table 1 . The three groups did not differ significantly at baseline visit in any additional medication (Table 1 ). During 1-year and 2-year follow-up the number of patients receiving medication and drug doses was unchanged (data not shown). For all types of medication, no significant changes or differences among the groups and the visits were observed. Patients under 0.5 mg/kg agalsidase-β tolerated switch to agalsidase-α well. In general, three (13.0%) patients of the former dose-reduction group developed mild infusion reactions, such as mild fever and increased fatigue, after infusion. These patients were premedicated with steroids and histamine antagonists before infusions.
Discussion
The agalsidase-β supply shortage between 2009 and 2012 forced the practitioners to treat the patients with FD with a reduced dose of agalsidase-β or to switch them to a regular-dose of agalsidase-α . In our initial study, comprising 105 patients with FD followed-up for 12 months, patients receiving regular agalsidase-β doses had a stable disease course, but both dose-reduction and switch to agalsidase-α resulted in worsening of renal function and symptoms.13
In this study, we assessed clinical stability and safety during agalsidase-β dose-reduction and/or switch to agalsidase-α in a 2-year follow-up with a special focus on clinical events, renal end organ damage, disease-related clinical symptoms, and disease severity score.
Our main findings are as follows: (1 ) patients with FD irrespective of the ERT regime showed a stable clinical disease course with respect to serious clinical events; (2 ) patients with FD under a regular agalsidase-β dose showed stable renal function and FD-related symptoms; and (3 ) patients with FD on a reduced agalsidase-β dose for 12 months and subsequent switch to a regular agalsidase-α dose for another 12 months and those patients with FD after a direct switch to agalsidase-α showed a significant decline in renal function, an increase of MSSI score, and a higher frequency of gastrointestinal pain.
ERT in FD
Recent studies with agalsidase-α and agalsidase-β have shown that ERT is safe and efficient for treatment of patients with FD.2 – 4 , 17 – 20 β supply was limited for 2.5 years,12 β or a switch to agalsidase-α for many patients. Limited clinical data on the basis of potentially statistically underpowered8 9 – 12 β or were switched to agalsidase-α , has been previous reported.12 13 α could be too low to guarantee results as effective as those of agalsidase-β .8
Here we report our results after additional 12 months of observation, with a special focus on renal outcome. Nephropathy is a dominant organ manifestation in FD, and progressive GFR decline may lead to ESRD in the third to fifth decade of life.21 16
The KDIGO 2012 Clinical Practice Guideline for the Evaluation and Management of Chronic Kidney Disease recommend use of the 2009 CKD-EPI equation14 22 creat . Second, they suggest use of the 2012 CKD-EPI equations15 cys or eGFRcreat-cys ) whenever cystatin C has been measured.16
The superior performance of cystatin C or the combination of creatinine and cystatin C versus creatinine alone to estimate GFR and predict CKD outcomes has been repeatedly demonstrated.15 , 23 – 25 cys and eGFRcreat-cys have a consistent linear association with increased risk of death for all eGFR levels below approximately 85 ml/min per 1.73 m2 , which is well above the threshold of 60 ml/min per 1.73 m2 for the detection of CKD with a creatinine-based eGFR.16 , 26 , 27 25 et al. mentioned that unmeasured and largely unknown non-GFR determinants of cystatin C are similar in magnitude to those of creatinine.15 15
Similar to patients with diabetes mellitus, most patients with FD are at high risk for CKD. In that respect, Perkins and colleagues demonstrated the accuracy of the cystatin C–based GFR equation to detect eGFR decline over time, especially in patients with diabetes with normal or even high GFR values28 25
Indeed, the primary goal of our study was to assess effects of the change or reduction of ERT on renal function between baseline and the 2-year follow-up visit and the discrimination of clinical outcomes. In this respect, we determined eGFR by the CKD-EPI formula on the basis of creatinine, cystatin C, and the combination of both. Patients on a regular agalsidase-β dose showed a stable renal function over 2 years irrespective of the CKD-EPI formula used. Because of ongoing agalsidase-β supply shortage we were forced to switch most patients formally receiving a reduced dose of agalsidase-β to a regular-dose of agalsidase-α . Patients under a reduced dose of agalsidase-β for 12 months and subsequent switch to a regular-dose of agalsidase-α for additional 12 months showed an eGFR decline irrespective of the three calculations used. In patients directly switched to agalsidase-α an adjusted annual eGFR decline with all three formulae was observed. This may indicate that directly switched patients suffer from a more pronounced renal impairment after 2-years follow-up. Besides the observed renal impairment, patients with FD presented with increasing frequencies of gastrointestinal pain and worsened MSSI scores in the dose-reduction-switch and switch groups. However, cardiac measurements remained stable in all three groups during the 2 years of follow-up.
In contrast with our findings after 1 year follow-up,13 29 , 30
Clinical Effect
As reported previously, the supply restriction for agalsidase-β caused distress and concern in both patients and physicians. Because of the observational study design, it cannot be concluded that the regular agalsidase-β dose regimen is the optimal treatment for all patients with FD. In addition, the observed eGFR decline in patients directly switched to agalsidase-α might not only be the result of a lower ERT dose, but it may also be the result of a more severe renal impairment in these patients when ERT was initiated. On the basis of our study, we conclude that whenever agalsidase-β is chosen, a dose-reduction and subsequent switch to agalsidase-α should be avoided. In addition, a direct switch from agalsidase-β to agalsidase-α is safe in general, but clinicians should be aware that eGFR may decline.
Limitations
Our study has some limitations, such as its observational nature and therefore an unavoidable selection of patients. Because of the observational design (real-world design), patients were not randomized. Therefore, outcomes of comparison between the three groups should be carefully interpreted. The total number of patients was slightly lower than the initial publication13 1 ) one inclusion criterion was the determination of a complete dataset at baseline and after 2 years and (2 ) patients switching the therapy regimen more than two times during the follow-up were excluded. Because of the multicenter approach, some parameters and especially lyso-Gb3 levels as a potential marker for disease progression were not available for the entire study cohort. The observed clinical response may be influenced by the development of inhibitory antibodies against ERT, which were not systematically assessed in our multicenter approach; however, a switch of product has no effect on their formation.31 32
Conclusions
After 2 years of observation, patients receiving a regular-dose of agalsidase-β had a stable disease course, whereas those with dose reduction and switch to agalsidase-α had an impairment of renal function.
Concise Methods
Study Design
In this retrospective observational study, a cohort of 89 patients (56 men, 33 women) with genetically confirmed FD from three German Fabry Centers in Berlin, Münster, and Würzburg were consecutively recruited and followed-up for 2 years. Patients had been on a stable treatment with agalsidase-β 1.0 mg/kg body wt e.o.w. for at least 1 year and reported at the Fabry centers for their regular clinical follow-ups. The routine clinical assessment included cardiac, renal, and neurologic parameters. The documentation of assessments followed clinical practice of the German Fabry Expert Centers for a rare multisystemic disorder. Inclusion criteria were as follows: (1 ) adult patients (≥18 years of age) with genetically determined FD, (2 ) at least 12 months stable treatment with a regular-dose of agalsidase-β 1.0 mg/kg body wt e.o.w., (3 ) 1- and 2-year follow-up visits at the respective FD centers after dose reduction and/or switch to agalsidase-α 0.2 mg/kg body wt e.o.w., and (4 ) informed consent for examinations and participation in the study. All investigations were performed after approval of the ethics committees of the participating centers (project no.: 2011–347-f), and written informed consent of the patients for molecular analysis and publication was obtained.
Patients changing therapy more often than twice after at least 12 months of stable treatment with the regular-dose of agalsidase-β 1.0 mg/kg body wt e.o.w. (inclusion criterion 2) were excluded. Our study comprised 89 instead of the initial 105 patients with FD because of dropouts and incomplete datasets for some parameters that were not available for the entire study cohort. The baseline visit was defined as the last visit when patients were still treated with the regular-dose of agalsidase-β 1.0 mg/kg body wt e.o.w. After this visit the long-term therapy strategy was determined individually by the treating physicians in accordance with the patient. The following options were proposed (Figure 1 ): (1 ) remain on the regular-dose of agalsidase-β of 1.0 mg/kg body wt e.o.w. (regular-dose group); (2 ) change to a reduced dose of agalsidase-β of 0.3 or 0.5 mg/kg body wt for 12 months and subsequently switch (ongoing supply shortage) to agalsidase-α with a standard dose of 0.2 mg/kg body wt e.o.w. (dose-reduction-switch group) for another 12 months; or (3 ) compound switch to agalsidase-α with a standard dose of 0.2 mg/kg body wt e.o.w. (switch group). The regular-dose group should not be considered as a control group but serves for a better understanding of the results obtained in the 2 core groups (dose-reduction-switch group and switch group). The determined long-term treatment was initiated, and patients were invited for follow-up visits (1- and 2-year follow-up) after 1 year of stable treatment with the implemented therapy. Therefore, in the final dataset, all data from three consecutive visits were included (baseline-visit, 1-year follow-up-visit, and 2-year follow-up-visit).
The detailed clinical work-up of patients has been previously reported.13 creat ),14 cys ),15 creat-cys )15 2 , eGFRcreat ) or under dialysis were excluded. All patients underwent neurologic examination and a clinical interview focusing on a history of strokes/transient ischemic attacks and pain.
Outcome Parameters
To quantify the clinical outcome, three groups of Fabry-related progression parameters were analyzed.
Clinical Events
For clinical events, the following parameters were analyzed: (1 ) death; (2 ) symptomatic cardiac arrhythmia requiring an implantable cardioverter defibrillator or pacemaker, myocardial infarction, coronary artery bypass graft, or percutaneous transluminal coronary angioplasty; (3 ) progression of renal disease to CKD stage 5 (eGFR <15 ml/min per 1.73 m2 [with at least 30% decrease of eGFR], kidney transplantation or dialysis); and (4 ) stroke/transient ischemic attack.
Change in Organ Function and/or Structure
For cardiac changes, end-diastolic septal and posterior wall thickness and ejection fraction were analyzed. For renal changes, eGFR and ACR in spot urine were analyzed. For neurologic changes, clinical examination and interview on stroke and transitory ischemic attacks were performed.
Change of Fabry-related Symptoms
Appearrance of gastrointestinal pain (abdominal pain, tenesmus, or cramping more than once a week), diarrhea (more than three loose bowels or >250 g of stool weight per day), hypohidrosis or anhidrosis (impairment or loss of ability to sweat), tinnitus, Fabry-associated pain (i.e., pain attacks, permanent pain or pain crises) was assessed; additionally the MSSI score33
Data Analyses
Data are presented as mean±SD, median (range), or number (%), where appropriate. Differences between groups and between baseline and follow-ups have been tested via one-way (Kruskal–Wallis) or repeated-measure (Friedman) ANOVA for continuous variables or Fisher’s exact test for categorical variables, including correction for multiple testing. Annual percentage changes in eGFRs were analyzed using one-sample t test. Annual changes (in ml/min per 1.73 m2 ) were adjusted for baseline eGFR values, age, sex, and premedication. The P values <0.05 were considered statistically significant. Data were analyzed using SAS version 9.3 (SAS Institute, Cary, NC) and GraphPad PRISM V5.0 software (GraphPad Software, La Jolla, CA).
Disclosures
F.W., J.K., M.L., C.W., T.D., S.C.K., and E.B. have received speaker honoraria from Genzyme and Shire Corporation. F.W. and C.W. are members of the Fabry Registry European Board of Advisors and have received travel assistance and speaker honoraria. Research grants were given to the institutions (Würzburg and Münster) by Genzyme and Shire Corporations. NÜ has received travel assistance and speaker honoraria from Genzyme Corporation and Shire Corporation. C.S. and J.S. have received speaker honoraria from Genzyme Corporation. S.M.B. has received speaker honoraria from Shire Corporation. H.W.H., D.B., and S.R. report no disclosures.
We thank Barbara Broll, Irina Turkin, Anne Huster, Samira Schiwek, and Jutta Beilker for expert technical assistance.
This study was funded by Genzyme Europe B.V. Eva Brand was supported by a Heisenberg professorship from the Deutsche Forschungsgemeinschaft (Br1589/8-2).
The funding agency had no role in the design and conduct of the study, in the collection, analysis, and interpretation of the data, or in the preparation, review, or approval of the manuscript. The researchers were independent of the funding agency.
Published online ahead of print. Publication date available at www.jasn.org
This article contains supplemental material online at http://jasn.asnjournals.org/lookup/suppl/doi:10.1681/ASN.2015030337/-/DCSupplemental
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