Base (HCO₃⁻/CO₃²⁻) Transport Properties of SLC4 Proteins: New Insights in Acid-Base Kidney Physiology

Abstract

H⁺ or base transporters and channels in the mammalian genome play important roles in the maintenance of numerous cellular biochemical and physiologic processes throughout the body. Among the known base transporters, those within the SLC4 and SLC26 gene families are involved in cell, transepithelial, and whole organ function. Whether the functional properties of these transporters involve HCO₃⁻, CO₃²⁻, or HCO₃⁻/CO₃²⁻ stimulated H⁺ (or OH⁻) transport has not received widespread attention in the literature. Accordingly, “bicarbonate” is the term typically used in most textbooks without greater specificity. Moreover, clinicians and physiologists have historically focused on the blood HCO₃⁻ concentration as the base term in the Henderson–Hasselbalch equation in the analysis of clinical acid-base abnormalities, thus, bicarbonate has been assumed to be the species reabsorbed along the nephron as required to maintain the blood [HCO₃⁻] at approximately 25 mM. However, accumulating data in the literature suggest that carbonate, rather than bicarbonate, is the species absorbed across the proximal tubule basolateral membrane, whereas in the collecting duct, bicarbonate is indeed transported. Various experimental approaches leading to this new concept are herein reviewed.