Pathophysiology of Renal Disease and ProgressionAntagonism of PDGF-D by Human Antibody CR002 Prevents Renal Scarring in Experimental GlomerulonephritisOstendorf, Tammo*; Rong, Song*; Boor, Peter*; Wiedemann, Stefanie*; Kunter, Uta*; Haubold, Ulrike*; van Roeyen, Claudia R.C.*; Eitner, Frank*; Kawachi, Hiroshi†; Starling, Gary‡; Alvarez, Enrique‡; Smithson, Glennda‡; Floege, Ju[Combining Diaeresis]rgen* Author Information *Divison of Nephrology, Rheinisch-Westfa[Combining Diaeresis]lische Technische Hochschule University of Aachen, Aachen, Germany; †Department of Cell Biology, Institute of Nephrology, Niigata School of Medicine, Niigata, Japan; and ‡CuraGen Corporation, Branford, Connecticut Address correspondence to: Dr. Tammo Ostendorf, Division of Nephrology, University Hospital Aachen, Pauwelsstrasse 30, D-52074 Aachen, Germany. Phone: +49-241-8089424; Fax: +49-241-8082446; E-mail: [email protected] Accepted January 25, 2006 Received July 8, 2005 Journal of the American Society of Nephrology 17(4):p 1054-1062, April 2006. | DOI: 10.1681/ASN.2005070683 Buy Metrics Abstract Glomerular mesangial cell proliferation and/or matrix accumulation characterizes many progressive renal diseases. PDGF-D was identified recently as a novel mediator of mesangial cell proliferation in vitro and in vivo. This study investigated the long-term consequences of PDGF-D inhibition in vivo. Rats with progressive mesangioproliferative glomerulonephritis (uninephrectomy plus anti–Thy-1.1 antibody) received the PDGF-D–neutralizing, fully human mAb CR002 on days 3, 10, and 17 after disease induction. Glomerular mesangioproliferative changes on day 10 were significantly reduced by anti–PDGF-D treatment as compared with control antibody. Eight weeks after disease induction, anti–PDGF-D therapy significantly ameliorated focal segmental glomerulosclerosis, podocyte damage (de novo desmin expression), tubulointerstitial damage, and fibrosis as well as the accumulation of renal interstitial matrix including type III collagen and fibronectin. Treatment with anti–PDGF-D also reduced the cortical infiltration of monocytes/macrophages on day 56, possibly related to lower renal cortical complement activation (C5b-9 deposition) and/or reduced epithelial-to-mesenchymal transition (preserved cortical expression of E-cadherin and reduced expression of vimentin and α-smooth muscle actin). In conclusion, these data provide evidence for a causal role of PDGF-D in the pathogenesis of renal scarring and point to a new therapeutic approach to progressive mesangioproliferative renal disease. Copyright © 2006 The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved.