BASIC SCIENCE: PDF OnlyIncrease in Percentage of CD45RO+/CD8+ Cells Is Associated with Previous Severe Primary HIV InfectionBruunsgaard, Helle; Pedersen, Court*; Scheibel, Elma†; Pedersen, Bente KlarlundAuthor Information †Department of Infectious Diseases and Haemophilia Centre, Rigshospitalet; and *Department of Infectious Diseases, Hvidovre County Hospital, University of Copenhagen, Denmark Address correspondence and reprint requests to Dr. Bente Klarlund Pedersen at the Department of Infectious Diseases M7721, Rigshospitalet, Tagensvej 20, DK-2200 Copenhagen N, Denmark. Manuscript received November 7, 1994; accepted February 2, 1995. Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology: October 1, 1995 - Volume 10 - Issue 2 - p 107-114 Free Abstract Summary: The purpose of the study was to examine how memory (CD45RO) and naive (CD45RA) phenotypes of CD4+ and CD8+ T-cell subpopulations changed with respect to progression and duration of human immunodeficiency virus (HIV) infection. Forty-three HIV-seropositive (HIV +) subjects with known time for seroconversion were included in this cross-sectional study. They were divided into the following groups for comparison: persons with and without AIDS, persons who had seroconverted >72 and <72 months before entering the study, persons with or without previous severe primary infection, persons who had developed AIDS >72 and <72 months before entering the study. Furthermore, the HIV + group was compared with an HIV- seronegative (HIV -) age- and sex-matched group. There was no difference in the proportion of total naive relative to total memory cells between HIV+ and HIV - subjects, showing an equal loss of naive and memory CD4+ cells in this study. Moreover, there was no difference in the proportion of total naive relative to memory CD8+ cells, showing an equal increase in both subgroups of CD8+ cells in HIV+ subjects. However, HIV+ subjects who had experienced severe primary symptoms resembled the AIDS group regarding shift in the CD8 phenotype from naive to memory and by down-regulation of amounts of CD45RA protein. Furthermore, the results showed that during infection with HIV the amounts of both CD45RA and CD45RO markers on CD4+ cells and CD45RA on CD8+ cells were down-regulated, although with different kinetics. Duration of HIV infection and time from seroconversion to AIDS were not reflected in the amount of or proportional expression of CD45RA and CD45RO markers. © Lippincott-Raven Publishers.