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Prevention Research

HIV Self-Testing Increases HIV Testing Frequency in High-Risk Men Who Have Sex With Men: A Randomized Controlled Trial

Katz, David A. PhD, MPH*,†; Golden, Matthew R. MD, MPH*,†,‡; Hughes, James P. PhD§; Farquhar, Carey MD, MPH*,‡,║; Stekler, Joanne D. MD, MPH*,‡

Author Information
JAIDS Journal of Acquired Immune Deficiency Syndromes: August 15, 2018 - Volume 78 - Issue 5 - p 505-512
doi: 10.1097/QAI.0000000000001709

Abstract

INTRODUCTION

HIV testing is the entry point into the HIV prevention and care continuum and is critical for reducing ongoing transmission and morbidity. However, traditional testing approaches are not reaching all persons with HIV. The US Centers for Disease Control and Prevention recommends at least annual testing for men who have sex with men (MSM), and many local health departments recommend more frequent testing for high-risk MSM to decrease time spent undiagnosed.1 However, many MSM continue to test infrequently, and estimates suggest that 14% of US MSM living with HIV remain unaware of their infection and potential for onward transmission.2

HIV self-testing, which allows testers to use rapid tests on self-collected specimens to learn their status at the time and location of their choosing, has long been proposed as a method to increase testing frequency and reach persons who would not otherwise test.3–6 The US Food and Drug Administration (FDA) approved the first HIV self-test, the OraQuick In-Home HIV Test, in 2012. However, concerns exist regarding the safety, accuracy, and public health impacts of these tests,5–10 and few controlled studies have been conducted to clarify the benefits and risks of HIV self-testing, particularly among MSM.11

Before approval of the first self-test, evidence from observational studies and cross-sectional surveys suggested that self-testing would be acceptable and feasible for MSM and would increase testing for individual MSM and within partnerships.7,12–24 However, the effect of self-testing on HIV testing frequency and the potential for the absence of risk-reduction counseling to lead to increased HIV risk had not been examined. To that end, we randomized 230 high-risk MSM to have access to HIV self-testing at no cost or to testing as usual to determine whether the availability of self-testing increases testing frequency without negatively impacting HIV risk behaviors.

METHODS

Design Overview

We conducted an open-label randomized controlled trial of HIV self-testing using the OraQuick ADVANCE Rapid HIV-1/2 Antibody Test (OraQuick; OraSure Technologies, Inc., Bethlehem, PA) versus standard of care for 15 months among MSM at high risk for HIV acquisition in Seattle, Washington, from September 2010 to December 2014. Ethical approval was obtained from the University of Washington (UW) Human Subjects Division. The protocol is available as Supplemental Digital Content 1, http://links.lww.com/QAI/B159. An investigational device exemption was received from the FDA for off-label use of OraQuick (BB-IDE 14354). Data were analyzed using Stata version 11 (College Station, TX).

Setting and Participants

In the Seattle area, HIV testing is available to MSM at multiple sites in the community at no charge or on a sliding scale, including the Public Health—Seattle & King County (PHSKC) STD Clinic and community-based organizations, as well as through private providers. PHSKC recommends quarterly HIV testing to MSM at high risk for HIV acquisition, defined as reporting at least one of the following in the previous year: condomless anal intercourse (CAI) with HIV-positive/unknown partners, diagnosis with bacterial sexually transmitted infection (STI), methamphetamine or inhaled nitrite use, or ≥10 male sex partners; and annual testing to lower risk MSM.25,26

The study enrolled people assigned male sex at birth and trans men, who met the following eligibility criteria: English-speaking, aged 18 years and older, reported sex with men in the year before enrollment, had a documented HIV-negative test ≤30 days before enrollment, at high risk per PHSKC, able to provide a stable mailing address, planning to live in the Seattle area for the duration of follow-up, and not participating in other studies with protocol-specified HIV testing at regular intervals. Only high-risk MSM were enrolled, so that study recommendations for quarterly testing were uniform among all participants and consistent with PHSKC guidelines.

Participants were recruited from the PHSKC STD Clinic and other Seattle sites serving MSM through advertisements and clinician referral; waiting room recruitment in the STD Clinic; advertisements on Facebook, Google Ads, and local MSM websites; and local LGBTQ listservs. Potential participants without documentation of a negative HIV test in the 30 days before enrollment were tested at an initial in-person study visit. HIV testing included screening through third-generation enzyme immunoassay (EIA) followed by pooled nucleic acid amplification testing (NAAT) for acute infection if EIA negative and Western blot confirmatory testing if EIA positive.27

Randomization and Intervention

Participants were assigned on a 1:1 basis to the intervention or control arm by variable block randomization; group assignment and block size were concealed from study staff involved in participant interaction until after enrollment. At enrollment, participants randomized to the intervention arm received in-depth training in the performance of OraQuick and a single test to take home. In the absence of an FDA-approved self-test at study initiation, study kits were designed to mirror the test under consideration based on discussions of the FDA's Blood Products Advisory Committee28,29 and included instructions, pre- and post-test counseling materials, a list of local HIV-related resources, and condoms. Instructions for providers developed by the UW HIV Web Study were adapted for unassisted self-testing.30 Additional kits were available on request and could be mailed or picked up at the clinic. To minimize the likelihood of test sharing, participants were limited to 1 kit per month unless needed to replace an invalid, damaged, or misplaced test. After one participant shared his kit with a partner who tested HIV positive,22 the Institutional Review Board required participants be informed that they would be taken off-study if they shared kits. Study staff maintained a 24-hour telephone contact for procedural questions, technical support, counseling, and scheduling confirmatory testing after reactive self-tests.

Study Procedures

Participants in both arms were required to complete an in-person enrollment visit and an end-of-study visit. At baseline, in the absence of a uniform standard of care for HIV testing, procedures encouraged testing per PHSKC guidelines: all participants were educated regarding acute infection and sites where NAAT was available; advised to test quarterly at a location of their choosing; given a card with recommended testing dates; and offered testing reminders through email, phone, or letter at a frequency of their choosing. Self-administered computer-based questionnaires completed at enrollment and end-of-study visits addressed sociodemographic characteristics, HIV testing, sexual behaviors, substance use, and intentions to use self-tests. To improve retention, all participants were contacted quarterly through email to complete an online survey to update their contact information. The 9-month contact update included questions regarding sexual behavior to improve power to assess the impact of self-testing on risk. At enrollment and end of study, participants were screened for HIV, syphilis, gonorrhea, and chlamydial infection. Participants who left the Seattle area and completed end-of-study procedures remotely were not screened for HIV/STIs.

Outcomes and Statistical Analyses

Primary Objective: Effects on HIV Testing Frequency

Participants were asked how many times they tested for HIV while enrolled in the study (excluding tests at study visits) as part of the end-of-study questionnaire. Participants completed event history calendars immediately before the questionnaire to assist with recall.31 We compared the mean number of HIV tests reported by participants in each arm using the t test. With a target effective sample size of 197 participants, the study had 84% power to detect a mean difference of 0.58 tests between the 2 arms. Secondary analyses used Pearson's χ2 tests to compare (1) the proportion of participants in each arm reporting compliance with PHSKC recommendations to test ≥4 times during follow-up and (2) the proportion of participants in each arm reporting ≥1 test. We estimated replacement of clinic testing with self-tests by subtracting the number of clinic HIV tests reported by men in the self-testing arm (calculated as the total number of tests minus the number of self-tests) from the number of HIV tests reported by men in the control arm. We also conducted an unplanned comparison of the mean number of STI tests reported by participants in both arms using the t test.

Secondary Objective: Effects on Sexual Behavior and STI Diagnosis

To address concerns that the absence of face-to-face risk-reduction counseling in self-testing would result in increased HIV risk, we designed our secondary objective to determine whether self-testing is noninferior to standard testing with respect to 3 measures of risk. Outcome definitions, assessment, prespecified noninferiority bounds, and statistical methods are described in Table 1.

TABLE 1.
TABLE 1.:
Ascertainment and Statistical Methods for Determining Noninferiority of HIV Self-Testing With Respect to Sexual Behavior and STI Acquisition

Validating Self-Reported Tests

We attempted to validate the total number of HIV tests and self-tests reported by participants in the end-of-study survey directly and indirectly. For clinic tests, we assessed where participants tested during follow-up and requested consent to obtain medical records for confidential tests. The number of confirmed clinic tests was defined as the number of separate clinical visits during follow-up at which an HIV test result was recorded. For self-tests, the number of confirmed tests was defined as the number of self-tests distributed to each participant minus the number participants reported never receiving, having missing or broken parts, sharing with nonparticipants, and remaining unused at the end of follow-up. Because participants had to actively request additional tests, we assumed that distributed tests that did not meet these criteria were used by participants. We compared the number of reported versus confirmed tests, overall and stratified by testing site and study arm, using paired t tests. Comparisons of overall and clinic testing were limited to participants who reported no clinic testing or reported all clinic testing as confidential (ie, name-based) and for whom records (or a confirmed absence of records) could be obtained for all testing sites. Comparisons of self-tests were limited to participants who reported the number of self-tests they performed and confirmed whether they had used all their self-tests at the end-of-study visit. Finally, we repeated the primary outcome comparison using confirmed tests only.

Acceptability, Ease of Use, and 24-Hour Contact Utilization

The enrollment questionnaire assessed anticipated effects of self-testing on testing frequency and, among the first 117 participants, amount willing to pay for a self-test and anticipated effects of cost on self-test use. Ease of use was assessed in the end-of-study questionnaire. Study staff maintained a log of calls to the 24-hour contact.

HIV Diagnosis and Incidence

Participants completing in-person end-of-study visits received HIV testing and were asked to release medical records for review to identify new infections, confirm reported diagnoses, and estimate dates of HIV infection. Incidence of HIV diagnosis was calculated by dividing the number of diagnoses by the total time from enrollment to first positive test or end-of-study visit and compared across study arms using Cox proportional hazards regression.

RESULTS

From September 2010 to September 2013, we screened 518 potential participants and enrolled 230 (44%) (Fig. 1). Most participants were white, college-educated, and male-identified and reported testing on a regular schedule (Table 2). At enrollment, men in the intervention arm were less likely to report having previously used a home HIV test and to test positive for a bacterial STI.

FIGURE 1.
FIGURE 1.:
Study flowchart.
TABLE 2.
TABLE 2.:
Characteristics of 230 Men Who Have Sex With Men Participating in a Randomized Controlled Trial of HIV Self-Testing at Baseline, by Study Arm

Of enrolled participants, 197 (86%) completed study follow-up with no differences in retention between arms [98 (84%) and 99 (87%) in the self-testing and control arms, respectively; P = 0.6]. Of these 197, 194 participants completed ∼15 months of follow-up, and 3 tested positive during follow-up, who completed an end-of-study visit at 3, 10, and 12 months. Median duration of follow-up for participants who completed the study was 15 months and did not differ by arm (P = 0.8). Of 33 who did not complete the study, 1 withdrew early and did not complete an end-of-study visit, and 32 were lost to follow-up. Being older, greater educational attainment and reporting testing regularly before enrollment were associated with study completion (P < 0.05 for all).

Effect of Self-Testing on HIV Testing Frequency

Among the 197 men who completed the study, men randomized to self-testing reported significantly more HIV tests during follow-up (mean = 5.3, 95% confidence interval (CI) = 4.7 to 6.0) than those in the control arm (3.6, 3.2 to 4.0; P < 0.0001), representing an average increase of 1.7 tests per participant over 15 months (95% CI = 0.9 to 2.5; Fig. 2). Almost all men reported testing at least once (98% self-testing arm vs. 93% control, P = 0.17), but men in the self-testing arm were significantly more likely to report at least quarterly testing as recommended (76% vs. 54%, respectively; P = 0.001). Among men randomized to self-testing, 77% reported testing more often during follow-up than they would have without access to self-testing, 22% as often, and 1% less often.

FIGURE 2.
FIGURE 2.:
Distribution of self-reported number of HIV tests during 15 months of follow-up among men who have sex with men participating in a randomized controlled trial of HIV self-testing, by study arm.

Of the 98 men randomized to self-testing who completed follow-up, 40 (41%) reported only using self-tests, 48 (49%) reported self-testing and clinic-based testing, and 10 (10%) reported only clinic-based testing. The mean number of self-tests reported was 3.9 (range = 0-16). Men randomized to self-testing tested at clinics an average of 1.4 times during follow-up (vs. 3.6 among controls; P < 0.0001), suggesting that they replaced an average of 2.2 clinic-based tests with 3.9 self-tests. Men in the self-testing arm reported significantly fewer STI tests during follow-up (mean = 2.3, 95% CI = 1.9 to 2.7) than men in the control arm (3.2, 2.8 to 3.6; P = 0.0038).

Effects of Self-Testing on Measures of HIV Risk

Self-testing was noninferior to clinic-based testing with respect to all 3 markers of HIV risk (Table 3). At the 9-month and end-of-study surveys, men in the self-testing arm reported 8% fewer male CAI partners in the previous 3 months than men in the control arm (incidence rate ratio = 0.92, 95% CI = 0.64 to 1.33). At the 9-month and end-of-study surveys, men randomized to self-testing were 1.07-fold more likely to report nonconcordant CAI in the previous 3 months than men in the control group (odds ratio = 1.07, 95% CI = 0.61 to 1.90). Of 183 who received STI screening at the end-of-study visit, 5.4% (5/93) of MSM randomized to self-testing were diagnosed with a bacterial STI compared with 12.2% (11/90) of control participants [risk difference = −6.8%; 95% CI = −16 to +1.6%].

TABLE 3.
TABLE 3.:
Effects of Access to HIV Self-Testing on HIV Risk During Follow-up

Validation of HIV Testing

There were 130 men (66% of total) for whom all HIV tests had the potential to be verified through medical record review or self-test distribution. In this group, the 69 men randomized to self-testing had significantly more confirmed tests during follow-up (mean = 4.7, 95% CI = 4.0 to 5.4) than the 61 in the control arm (2.5, 2.1 to 2.9; P < 0.0001), representing 2.2 additional tests per participant over 15 months (95% CI = 1.4 to 3.1). Overall, men reported a greater number of tests than could be confirmed (mean reported = 4.5 vs. confirmed = 3.6; P < 0.0001). This difference was greater among men randomized to standard testing (4.0 vs. 2.5; P < 0.0001) than self-testing (4.9 vs. 4.7; P = 0.32). Men in the self-testing arm significantly over-reported self-test use (mean reported = 4.0 vs. distributed = 3.4; P = 0.0018) but were fairly accurate when reporting clinic-based tests (mean reported = 1.8 vs. confirmed = 1.9; P = 0.64).

Acceptability, Ease of Use, and 24-Hour Contact Utilization

At enrollment, 99 (85%) of 117 men expected that they would test for HIV more often if they had access to self-tests. Only 15% reported that they would pay $40 or more for a self-test, 27% would pay $20–$40, 33% would pay $10–$20, 13% would pay less than $10, and 12% would only use a self-test if it was free. How often men believed they would use a self-test to test themselves for HIV infection depended on the cost; 87% expected to test 4 or more times per year if the test cost $5 compared with 23% if it cost $50 (McNemar's test: P < 0.001).

Almost all 88 men who reported using a self-test during follow-up reported, the test was very easy to use (94%) and being very sure they used it correctly (93%). However, the package insert indicates that the test requires ≥20 minutes to develop, and 9% of men who self-tested reported that, the last time they self-tested, they spent <20 minutes from opening the kit to test interpretation. The 24-hour contact was only used for requesting new kits, not for counseling or help using the test.

New HIV Diagnoses

Of 178 participants who received HIV testing at their end-of-study visit, 6 (3.4%) were HIV positive, 4 in the self-testing arm and 2 in the standard testing arm. Incidence of HIV diagnosis was 2.75 per 100 person-years (95% CI = 1.23 to 6.11; compared with an estimated HIV incidence of 0.5 per 100 person-years among MSM overall32) and did not differ by arm (P = 0.4). Of the 4 in the self-testing arm, one sought care with his primary care provider for symptoms of primary infection after the recent HIV diagnosis of his main partner. He reported self-testing positive in the week after this provider visit and before receiving laboratory test results including a viral load >1 million. Of the other 3, one reported his last negative test as a self-test 2 months before diagnosis at the end-of-study visit; one reported a negative self-test approximately 1 month before receiving his diagnosis at a routine physical examination, during which his provider noted signs of primary infection and his rapid HIV test was reactive; and one reported a negative self-test 5 days before a previously scheduled clinic test that indicated primary infection. All were linked to HIV care.

DISCUSSION

This study, among the first of its kind to evaluate the implementation of self-testing, demonstrated that access to HIV self-testing at no cost increased testing frequency among high-risk MSM in Seattle and had no effect on STI acquisition or sexual risk behaviors. However, the HIV testing histories of the few men who became infected during the study suggested that the availability of self-tests did not result in earlier HIV diagnosis, and participants enrolled in the self-testing arm had fewer tests for bacterial STIs in an environment with increasing rates of syphilis and gonorrhea.33

Our results are similar to a recently published trial among urban Australian MSM that found access to self-testing led to a 2-fold increase in the number of HIV tests reported in the previous year (an absolute increase in 2.1 tests) and had no effect on CAI with casual partners.34 It is encouraging that, in both settings and among MSM with varying histories of HIV testing, both studies observed large increases in testing frequency despite high levels of testing in the control groups (approximately every 4–5 vs. 6 months in the Seattle and Australian trials, respectively), and that neither study observed increases in indicators of HIV risk in the absence of counseling. However, in a setting where most men test frequently,35 we were unable to confirm whether self-testing had a large effect on HIV testing frequency among nonrecent testers. The potential for self-testing to reach non-testers or infrequent testers may provide unique population- and individual-level benefits, an issue we are exploring using mathematical modeling. In addition, in contrast to the Australian trial where self-testing seems to have supplemented clinic-based testing and did not affect STI testing, we found evidence of significant replacement of clinic-based tests with self-tests and a decrease in reported STI testing frequency among MSM in the self-testing arm. Our previous modeling work suggested that replacing clinic-based testing with self-testing in settings where clinics provide antigen–antibody tests or pooled NAAT has the potential to increase HIV transmission because the long window period of the FDA-approved self-test makes it unable to diagnose persons during early infection when they are most infectious.36 Research is needed to understand the differences in these results and how self-testing affects clinic-based HIV/STI testing in different settings as well as to develop and evaluate self-tests able to detect early HIV infection. Fingerstick HIV self-tests, which may have greater sensitivity and shorter window periods,37 have been introduced in some settings and studies38–41; however, the effects of access to oral fluid self-tests on HIV testing may not translate directly to fingerstick self-tests, which may differ in acceptability and usability.38,42

The study has several limitations. First, it was conducted among high-risk MSM in Seattle who agreed to participate in a self-testing trial and may not be generalizable to other settings or populations. Second, testing and sexual behavior outcomes relied on self-report and were therefore subject to recall error and selection bias. This is of particular concern because we were unable to blind participants to study arm. Comparing only confirmed tests yielded an even stronger association between self-testing and increased testing than the primary analysis, although this analysis was also sensitive to the limitations of directly measuring self-testing. In addition, the trial was powered for the primary outcome of HIV testing frequency and had limited power for noninferiority and HIV diagnosis–related outcomes, and STI acquisition and sexual risk behaviors do not always correlate directly with HIV risk.43,44 The OraQuick In-Home HIV Test became available for purchase during the study and may have affected participants' testing behaviors, particularly with respect to self-testing. However, the intervention effect remained consistent before and after its introduction (see Supplemental Digital Content 2, http://links.lww.com/QAI/B159). Adapting the professional OraQuick for self-testing instead of using FDA-approved self-tests may have affected self-test acceptability and performance. The trial was also conducted before widespread availability of pre-exposure prophylaxis (PrEP) in Seattle, and our results might differ in a setting with frequent PrEP use, where oral fluid testing is actively discouraged because of poor sensitivity in early infection45,46 and delayed seroconversion in oral fluids among PrEP users.47 Finally, randomization did not result in complete balance of measured potential confounders between arms. However, controlling for unbalanced factors did not affect estimates of the effects of self-testing on testing or risk (see Supplemental Digital Content 3 and 4, http://links.lww.com/QAI/B159).

Self-testing has great potential to increase awareness of HIV status among MSM, particularly if it can reach those who have never tested or test infrequently. Among men who already tested for HIV, self-testing can be used to supplement clinic-based testing and encourage routine or more frequent testing. How best to accomplish these goals is as yet unknown, but a number of strategies are emerging as feasible and acceptable to MSM,48 including through geosocial networking apps,49 bathhouses,50 self-test vending machines,51 vouchers for free self-tests,52 mass distribution online or at LGBTQ-focused events,53,54 and distribution by sex partners.20,38 It will be important to develop strategies for matching the correct self-test distribution models to the appropriate populations and settings and to the capacity of the implementing agency.

In summary, although our results demonstrate the potential benefit of HIV self-tests, much work remains to be performed. Research is needed to understand how to prioritize supplementation and reach new testers, to educate men about the limitations of the current self-test, and to invest in the development and evaluation of self-tests able to detect early infection. The potential for HIV self-testing to decrease STI testing despite a growing STI epidemic among MSM also points to the need to develop, evaluate, and integrate STI tests into HIV self-testing programs and products. To maximize the individual- and population-level benefits of self-testing, research is necessary to evaluate and ensure timely linkage to HIV care and prevention, including PrEP, after self-testing and ultimately to identify the most cost-effective methods of implementing self-testing in high-risk populations.

ACKNOWLEDGMENTS

The authors thank the study participants and study staff. They also thank the Public Health—Seattle & King County HIV/STD Program and Seattle LGBTQ community-based organizations and health care providers for their support and the Northwest AIDS Education & Training Center for their assistance in creating instructions for the HIV self-test.

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Keywords:

men who have sex with men; HIV self-testing; sexual behavior; HIV screening; sexually transmitted infections

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