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Strong Correlation Between Concentrations of Antiretrovirals in Home-Collected and Study-Collected Hair Samples

Implications for Adherence Monitoring

Saberi, Parya PharmD, MAS; Neilands, Torsten B. PhD; Ming, Kristin BS; Johnson, Mallory O. PhD; Kuncze, Karen BS; Koss, Catherine A. MD; Gandhi, Monica MD, MPH

JAIDS Journal of Acquired Immune Deficiency Syndromes: December 1, 2017 - Volume 76 - Issue 4 - p e101–e103
doi: 10.1097/QAI.0000000000001492
Letters to the Editor
Free

Department of Medicine, University of California, San Francisco, San Francisco, CA

Research reported in this publication was supported by the National Institute of Mental Health of the National Institutes of Health under Award Number Award Numbers R21MH108414 (P.S.), K23MH097649 (P.S.), and K24DA037034 (M.O.J.), the National Institute of Allergy and Infectious Diseases R01AI098472 (M.G.), and the Eunice Kennedy Shriver National Institute of Child Health and Human Development K12HD052163 (P.I. Brindis, Adler to support Koss). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

The authors have no funding or conflicts of interest to disclose.

To the Editors:

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BACKGROUND

Suboptimal adherence to either HIV therapy or pre-exposure prophylaxis (PrEP) increases the risk of virologic failure, drug resistance, and HIV transmission or acquisition.1–3 Hair concentrations of antiretrovirals (ARVs), which reflect cumulative exposure over weeks to months, are more strongly predictive of virologic response than self-reported adherence in HIV treatment4–6 and predict toxicities7 and dosing patterns8 among individuals on PrEP.

Measuring hair concentrations of ARVs to estimate adherence and exposure has several advantages, including lower susceptibility to “white coat adherence” patterns9 and the ability to estimate long-term adherence.10 Hair samples are collected noninvasively, do not require sterile equipment, and can be stored and shipped at room temperature and without biohazardous precautions.

In studies examining hair concentrations of ARVs to date, trained staff have collected hair samples from the back of participants' heads. However, just as self-collection of genital swabs can facilitate testing for sexually transmitted infections,11 home collection of hair samples may increase recruitment and retention in research because of reduced travel time and expense. In addition, because hair is more readily self-sampled from the side of the head, confirming ARV concentrations are similar across the scalp would bolster the case for home collection.

We conducted a pilot study to (1) evaluate the feasibility and acceptability of home collection of hair samples and (2) examine the correlation and agreement between ARV levels in hair collected at a study site by staff and hair collected at home by participants, as well as the correlation and agreement between ARV levels in hair collected from the back versus the side of the head.

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METHODS

Participants and Sample Collection

We recruited participants ≥18 years of age on tenofovir (TFV) disoproxil fumarate (TDF)/emtricitabine (FTC)-containing ARV regimens for at least 3 months. TDF/FTC is the backbone for ARV regimens and the combination used for PrEP and has the most comprehensive data on dose–hair concentration relationships.8 Participants with renal insufficiency were excluded.

Individuals who met study criteria were emailed a video link (http://rxpix.ucsf.edu/study-archives) describing the overall study objectives and demonstrating home collection of hair. Consenting participants attended study visits at University of California, San Francisco (UCSF), at baseline, 6, and 12 weeks. Trained staff collected hair samples from the back and side of participants' heads, at these 3 time points. At 6 and 12 weeks, we mailed the participants hair collection kits that included detailed instructions for home collection of hair (http://rxpix.ucsf.edu/study-archives), supplies for hair sample collection, and a prestamped addressed envelope to mail the hair samples back to study staff. Participants were instructed to collect hair samples from the back and side of their heads and ask for assistance from a friend or family member if desired. The study protocol was approved by the UCSF Institutional Review Board.

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Laboratory Methods

The UCSF Hair Analytical Laboratory (HAL) has developed, validated, and reported on methods to analyze TFV, FTC, and other ARVs in small hair samples using liquid chromatography/tandem mass spectrometry.5,10,12,13 The laboratory's hair assays for TFV and FTC have been peer reviewed and approved by the Division of AIDS Clinical Pharmacology and Quality Assurance program.14 Detailed methods for hair analyses have been previously described.8

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Data Analysis

Feasibility of home collection of hair samples was defined as the proportion of participants who mailed back adequate hair samples to the study site. Acceptability of home collection was evaluated through qualitative exit interviews focused on participants' opinions on home collection of hair samples.

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Qualitative Data

The first and third authors identified themes from exit interview and entered them into a matrix using Microsoft Excel where columns and rows represented themes and participants, respectively, to facilitate data analysis.15 The third author categorized each interview using this matrix, and coding was reviewed for accuracy by the first author.

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Quantitative Data

Univariate analyses were conducted to examine demographics, feasibility, and acceptability variables. In study-collected and home-collected hair samples from the back and side of the head, we examined (1) Pearson correlation coefficients to determine relative correlation between log10-transformed TFV and FTC levels, (2) concordance correlation coefficients to evaluate the agreement (ie, reproducibility) between hair levels, and (3) the Bland–Altman limits of agreement to estimate measurement bias. We used Stata 14 (StataCorp, College Station, TX) for analyses.

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RESULTS

We collected baseline data on 8 individuals who met entry criteria. One participant withdrew after the baseline visit because of life circumstances unrelated to the study and another participant withdrew at 12 weeks because of a family emergency. Participants had a mean age of 50.8 years, were 88% male, and 38% African American.

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Feasibility

Of the 26 home-collected hair samples that were expected from enrolled participants, we received 24 samples. One participant did not mail the 6-week home-collected samples, citing “chaos in life.” Of the 42 study-collected hair samples that were expected from enrolled participants, we collected 40 samples. One participant did not attend the 12-week study visit because of an infection but mailed back the home-collected hair samples and completed the exit interview by telephone.

Study staff received the home-collected samples a mean of 9 days from the date that hair kits were mailed, and all samples were in good condition for hair analysis. The HAL was unable to report TFV levels in 2 hair samples—both collected by study staff—because of a matrix effect (ie, unknown variables interfering with the assay).

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Acceptability

During recruitment, only 1 person did not participate because of not wanting to cut their hair. Six individuals were unable to participate because of baldness (N = 3) or having a finely shaved head (N = 3). All 8 individuals who participated stated that they had a positive study experience, found home collection of hair easy, and reported that they would participate in a similar future study.

“I just reached down and grabbed me a good little piece and felt it, looked in the mirror, clipped it, held it, put it on there, then taped it, folded it up, and sent it to you all.” (56-year-old African American transgender woman).

“It depends on an individual, how attached they are to a little piece of hair or not. But it's going to grow back, my god, it's not like chemo where it all fell out or something. They just have to let go of a little hair, that's all.” (57-year-old multiracial man).

“At home is a lot easier than actually having to come out here every month. Because the same thing you did here every month, I do it at home.” (50-year-old White man).

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Correlation and Agreement

As shown in the Table 1, hair levels of TFV and FTC, regardless of the region of the head or whether collected by staff and at home, had high correlation and agreement with each other (all Pearson correlation coefficients ≥0.85 and concordance correlation coefficients ≥0.83). FTC concentrations revealed a slightly higher correlation among the samples collected (correlation coefficients ≥0.95) than TFV concentrations in various samples (correlation coefficients ≥0.85). There was no evidence of measurement bias (mean differences ≤0.08).

TABLE 1

TABLE 1

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DISCUSSION

We demonstrated that home collection of hair is feasible and acceptable and yields ARV concentrations that were essentially equivalent to concentrations from study-collected samples. Participants reported that the home collection of hair samples was easy and unimposing. There was a high degree of correlation and agreement between ARV levels in hair collected by trained staff and at home by participants—and between hair collected from the back and side of the head—without any evidence of measurement bias.

This is the first study to examine the potential for home collection of hair for adherence monitoring. Our findings suggest this pharmacologic measure of adherence can be collected without the need for study visits, thereby broadening the reach of this biomarker (ie, hair levels) to diverse geographies and participant groups. This is also the first study to show that ARV levels from hair collected from the side of the head (an area easier to reach for home collection) is comparable with hair concentrations collected from the back of the head (the region from which research samples are typically collected). Finally, the correlation between FTC hair levels in samples collected at home versus those collected in the clinic was higher than the correlation for TFV in each set of samples. Therefore, if patients are switched from TDF/FTC to TFV alafenamide/FTC, hair concentrations of FTC can continue to serve as a reliable adherence biomarker.

Study limitations include a small sample size from a single geographic area and a focus on 2 ARVs (TDF/FTC). A larger 6-month study to examine the feasibility and acceptability of home collection of hair samples among participants taking other ARVs from various geographic regions across the US is in progress.

Adherence monitoring for PrEP during this implementation period and for achieving the UNAIDS “90:90:90” targets worldwide is essential. Hair ARV concentrations are predictive of future virologic failure4 and, if measured economically, may reduce the need for frequent viral load monitoring. Home collection of samples and remote monitoring can reduce missing data, decrease visit burden for research participants and clinic patients, enhance research participation of traditionally “hard-to-reach” populations, and increase generalizability of results. Home collection of hair samples may be an important step to expanding objective adherence monitoring in the context of HIV treatment and prevention worldwide.

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ACKNOWLEDGMENTS

The authors thank the participants for taking part in this study; Dr. Howard Horng, the HAL Director, and Alexander Louie in the HAL, for their work on the assays; Dr. Nicholas Sheon for his help in creating the video describing the study objectives and demonstrating home collection of hair; and Dr. Peter Baccetti for his guidance on the statistical analysis of hair ARV concentration data.

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