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123 Molecular Mechanisms of HIV Latency

Verdin, Eric M.D.

JAIDS Journal of Acquired Immune Deficiency Syndromes: April 2011 - Volume 56 - Issue - p 49
doi: 10.1097/01.qai.0000397311.17213.2f
Abstracts
Free

Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA

The presence of latent reservoirs has prevented the eradication of Human Immunodeficiency Virus (HIV) from infected patients. The mechanism of postintegration latency is poorly understood, partly because of the lack of an in vitro model. We previously reported that the chromatin environment at the site of integration of HIV into the cell genome plays an important role in its transcriptional activity. We also reported on the use of an HIV molecular clone expressing green fluorescent protein (GFP) to highly enrich for latently infected cells after infection. HIV latency occurred reproducibly, albeit with low frequency (1.5%), during infection. Clonal cell lines derived from this latent population showed no detectable basal expression, but could be induced fully after treatment with a variety of biological and pharmacological agents. I will discuss our study of these clonal cell lines and the mechanisms responsible for the establishment, the maintenance and the reactivation of latent HIV in this system.

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