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Famooto Oluranti; Olawande, Oluwatoyosi; Okuma, James; Dareng, Eileen; Adebamowo, Sally; Adebamowo, Clement
JAIDS Journal of Acquired Immune Deficiency Syndromes: January 2016
doi: 10.1097/01.qai.0000479741.67125.ad
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Background:There is need for accurate genotyping of Human Papillomavirus (HPV) infections because persistent infection with High- Risk HPV (hrHPV) is a necessary cause for CIN2+. It is essential therefore to evaluate various genotyping assays that have been developed for this very important endeavor within the context of epidemiological studies of HPV infections in different parts of the world. In this study, we compare HPV genotyping using the SPF10 DEIA LiPA25 version 1 with Roche Linear Array HPV genotyping assays in Nigerian women attending cervical cancer screening program.

Methods:One hundred and five women were tested for HPV infection using Roche Linear array HPV genotyping test, a PCR amplification technique on target DNA followed by hybridization using reverse line blot system and SPF10 DEIA genotyping system followed by LiPA25 version 1 genotyping, a 28 oligonucleotide probes that recognize 25 different types tailed with poly (dT) and immobilized as parallel lines onto membrane strips.

Results:HPV genotypes were detected in 36% (38/105) of the samples using SPF10 and 23% (24/105) of the samples using linear array. Some 65% (68/105) samples showed absolute agreement between the assays (concordant), 27% (28/105) samples were discordant while 9% (9/105) samples showed correspondence for some but not all genotypes detected on both strips (compatible).

Conclusions:There was 65% absolute agreement between the two assays and the SPF10/LiPA assay detected more HPV infections than the linear array.

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