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Sliepen Kwinten; Ozorowski, Gabriel; Burger, Judith; van Montfort, Thijs; Stunnenberg, Melissa; Bontjer, Ilja; LaBranche, Celia; Montefiori, David; Moore, John; Ward, Andrew; Sanders, Rogier
JAIDS Journal of Acquired Immune Deficiency Syndromes: January 2016
doi: 10.1097/01.qai.0000479550.29238.84
Abstract: PDF Only

Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell receptor cross-linking and B cell activation. We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664) to improve upon the promising neutralizing antibody responses obtained with BG505 SOSIP.664 alone. With the aim of increasing neutralization breadth we also generated SOSIP.664-ferritin nanoparticles from different viral strains from subtypes A, B and C. Trimer- bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer- bearing nanoparticles induced significantly higher neutralizing antibody responses than when the same trimers were delivered as soluble proteins. Thus, nanoparticle-displayed native-like trimers might be valuable in the quest for an HIV-1 vaccine aimed at inducing broadly neutralizing antibodies.

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