In order to identify HIV-1 subtypes circulating in Tunisia, blood specimens from 25 HIV-1 nfected Tunisian patients were collected. Proviral HIV-1 DNA was genotyped by sequence analysis of the C2-V3 env region. HIV-1 subtypes were determined in 21 DNA sequences: 20 were of subtype B and one was a circulating recombinant form (CRF02 AG).Subtype B largely dominates the epidemiology of HIV-1 infection in Tunisia, suggesting the probable imported origin of HIV-1 infection, but further studies are needed to investigate the potential diversification of HIV-1 isolates in Tunisia.
Address correspondence and reprint requests to Christophe Pasquier, Laboratoire de Virologie, Hôpital Purpan, Place Baylac, 31059 Toulouse Cedex, France; e-mail: email@example.com
Manuscript received January 8, 2001; accepted June 1, 2001.
© 2001 Lippincott Williams & Wilkins, Inc.