CLINICAL SCIENCE: PDF OnlyAnalysis of HIV-1 Reverse Transcriptase Gene Mutations in Infected Children Treated With ZidovudineOrlandi, Paola*; Cancrini, Caterina*; Scaccia, Stefania*; Romiti, Maria Luisa*; Livadiotti, Susanna*; Gattinara, Guido Castelli*; Angelini, Federica*; Cox, Susan†; Rossi, Paolo‡Author Information *Division of Immunology and Infectious Diseases, Children's Hospital Bambino Gesú, University of Rome “Tor Vergata,” Rome, Italy; †Medivir AB, Huddinge, Sweden; and ‡MTC Karolinska Institute, Stockholm, Sweden Address correspondence and reprint requests to Paolo Rossi, Children's Hospital Bambino Gesú, University of Rome “Tor Vergata,” Piazza San Onofrio 4, 00165 Rome, Italy; email: [email protected]. Manuscript received May 19, 1997; accepted May 19, 1998. Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology: November 1, 1998 - Volume 19 - Issue 3 - p 230-237 Free Abstract Summary: Prolonged treatment with antiretroviral agents directed against reverse transcription (RT) in patients with HIV-1 infection results in the emergence of virus variants with reduced sensitivity containing mutations in the HIV-1 RT gene. Development of zidovudine (ZDV)-related mutations was studied in a cohort of 24 vertically infected pediatric patients receiving ZDV therapy. Monthly clinical and immunologic evaluation was accompanied by direct sequencing of the HIV-1 RT gene every 4 months. A correlation was observed between the emergence of mutations and the duration of therapy. Mutation at codon 41 was found only in the presence of mutation at codon 215. The presence of the mutations Met41→Leu and Thr215→Tyr/Phe did not appear to be related to disease progression. These findings suggest that the mere presence of mutations in the HIV-1 RT gene alone during ZDV monotherapy is not a reliable prognostic marker in the absence of other clinical and virologic information. © 1998 Lippincott Williams & Wilkins, Inc.