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Chemokines Are Present in the Genital Tract of HIV-Seropositive and HIV-Seronegative Women: Correlation With Other Immune Mediators

Spear Gregory T.; Sha, Beverly E.; Saarloos, Mary Nel; Benson, Constance A.; Rydman, Robert; Massad, Stewart L.; Gilmore, Ruth; Landay, Alan L.
JAIDS Journal of Acquired Immune Deficiency Syndromes: August 15th, 1998

In this cross-sectional study, 53 cervicovaginal lavage samples (CVL) from 41 women were analyzed for the chemokines interleukin-8 (IL-8), regulated-onactivation normal T-expressed and secreted (RANTES) factor, and macrophage inflammatory protein-1α (MIP-1α) by enzyme-linked immunosorbent assay (ELISA). IL-8 was detected in 81% of CVL, whereas RANTES was detected in 32%, and MIP-1α in 15% of the CVL. The mean levels of IL-8, RANTES, and MIP-1α in positive samples were 396 pg/ml, 102 pg/ml, and 34 pg/ml, respectively. IL-8 levels correlated positively with IL-1β and IgG in a subset of CVL samples. RANTES levels correlated positively with complement protein levels. Additionally, the levels of RANTES, but not MIP-1α, reached levels reported in previous studies of the effects of β chemokines to inhibit HIV replication. These results suggest that measuring chemokines in CVL specimens can provide important information regarding immune responses in the genital tract.

Address correspondence and reprint requests to Gregory T. Spear, Department of Immunology/Microbiology, Rush-Presbyterian-St. Luke's Medical Center, 1653 West Congress Parkway, Chicago, IL 60612, U.S.A.

Manuscript received September 29, 1997; accepted March 16, 1998.

© Lippincott-Raven Publishers.