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Amino Acid Sequence Requirements for the Incorporation of the Vpx Protein of Simian Immunodeficiency Virus into Virion Particles

Park In-Woo; Sodroski, Joseph
JAIDS Journal of Acquired Immune Deficiency Syndromes: December 5th, 1995
BASIC SCIENCE: PDF Only

Summary:To investigate the amino acid sequence determinants for the incorporation of Vpx protein into virion particles, several mutations were introduced into the vpx gene of SIVmac239 proviral DNA, and the effects of mutations on the expression and assembly of the Vpx protein were studied. The results show that deletions of amino acids from 78 to 80 or from 82 to 87 abolished the incorporation of the expressed Vpx protein into virion particles. Other Vpx mutants, including a full-length Vpx-Vpr fusion protein and a mutant with a deletion of the C-terminal polyproline tract, were packaged efficiently. This study suggests that amino acids from 78 to 80 and 82 to 87 are important for the assembly of the Vpx protein into virus particles.

*Division of Human Retrovirology, Dana-Farber Cancer Institute; †Department of Pathology, Harvard Medical School; and ‡Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts, U.S.A.

Address correspondence and reprint requests to Dr. J. Sodroski at Dana-Farber Cancer Institute, JF 824, 44 Binney Street, Boston, MA 02115, U.S.A.

Manuscript received May 15, 1995; accepted May 24, 1995.

© Lippincott-Raven Publishers.