Technical NoteReconstruction and Visualization of Fiber and Laminar Structure in the Normal Human Heart from Ex Vivo Diffusion Tensor Magnetic Resonance Imaging (DTMRI) DataRohmer, Damien MS; Sitek, Arkadiusz PhD; Gullberg, Grant T. PhDEditor(s): Runge, Val M. MD Author Information From the Life Sciences Division, Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, California. Received July 21, 2006, and accepted for publication, after revision, May 21, 2007. Arkadiusz Sitek, PhD, is currently at the Department of Radiology, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts. Damien Rohmer is currently at Laboratoire Jean Kuntzmann, Institut National Polytechnique de Grenoble, Grenoble, France. Supported by the Director, Office of Science, Office of Biological and Environmental Research, Medical Science Division of the US Department of Energy under Contract No. DE-AC03-76SF00098 and in part by Public Health Service grant number R01EB000121 awarded by the National Institute of Biomedical Imaging and Bioengineering, Department of Health and Human Services. Reprints: Grant T. Gullberg, PhD, Lawrence Berkeley Laboratory, 1 Cyclotron Road, MS55R0121, Berkeley, CA 94720. E-mail: [email protected]. Investigative Radiology: November 2007 - Volume 42 - Issue 11 - p 777-789 doi: 10.1097/RLI.0b013e3181238330 Buy Metrics Abstract Objective: The human heart is composed of a helical network of muscle fibers organized to form sheets that are separated by cleavage planes responsible for the orthotropic mechanical properties of cardiac muscle. The purpose of this study is the reconstruction and visualization of these structures in 3 dimensions. Methods: Anisotropic least square filtering followed by fiber and sheet tracking techniques were applied to diffusion tensor magnetic resonance imaging data of the excised human heart. Fibers were reconstructed using the first eigenvectors of the diffusion tensors. The sheets were reconstructed using the second and third eigenvectors and visualized as surfaces. Results: The fibers are shown to lie in sheets that have transmural structure, which correspond to histologic studies published in the literature. Quantitative measurements show that the sheets as appose to the fibers are organized into laminar orientations without dominant populations. Conclusions: A visualization algorithm was developed to demonstrate the complex 3-dimensional orientation of the fibers and sheets in human myocardium. © 2007 Lippincott Williams & Wilkins, Inc.