The study aimed to investigate through histology, histometry, and immunohistochemistry the vital bone formation after grafting of biphasic calcium phosphate bioceramic (BC) in combination with calvarial autogenous bone into the dental sockets of rats.
Forty-five male rats were submitted to upper right incisor extraction and divided according to the grafted material in: control, bioceramic (BC), and bioceramic + autogenous bone (BC + AB). The animals were killed 7, 21, and 42 days after surgery for histological, histometric, and immunohistochemistry analysis.
Histomorphometric results demonstrated, for BC + AB group, formation of trabecular bone between the particles of BCs and autogenous bone, connecting them, as well as higher percentage of vital bone in comparison with BC. Immunohistochemical reactions showed intense labeling for Runx2-positive cells in the group BC + AB.
Autogenous bone was able to stimulate bone turnover enabling a larger amount of vital bone synthesis and can be recommended as a viable grafting material in combination with synthetic biphasic BC.
*Associate Professor, Department of Dentistry, Positivo University, Curitiba, PR, Brazil.
†Associate Professor, Department of Stomatology, Public Health and Forensic Dentistry, School of Dentistry of Ribeirão Preto, USP—University of São Paulo, Ribeirão Preto, SP, Brazil.
‡Associate Professor, Department of Basic Sciences, School of Dentistry of Aracatuba, UNESP, Universidade Estadual Paulista, Araçatuba, SP, Brazil.
§Consultant at The Royal London Dental Hospital, Department of Restorative Dentistry, Barts and The London NHS Trust; Honorary Senior Clinical Lecturer at Queen Mary University of London, Barts and The London School of Medicine and Dentistry; Chairman of the International Team for Implantology (ITI) UK & Ireland Section.
¶Professor, Department of Stomatology, Public Health and Forensic Dentistry, School of Dentistry of Ribeirão Preto, USP—University of São Paulo, Ribeirão Preto, SP, Brazil.
Reprint requests and correspondence to: Rander Moreira Macedo, PhD, Departamento de Odontologia, Universidade Positivo, Av. Prof. Pedro Viriato Parigot de Souza, 5300, Curitiba, PR, CEP 81280-330, Brazil, Phone: +55-41-3317-3180, Fax: +55-41-3317-3082, E-mail: email@example.com