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Conditioned Medium From Mesenchymal Stem Cells Enhances Early Bone Regeneration After Maxillary Sinus Floor Elevation in Rabbits

Katagiri, Wataru DDS, PhD; Osugi, Masashi DDS, PhD; Kinoshita, Kazuhiko DDS, PhD; Hibi, Hideharu DDS, PhD

doi: 10.1097/ID.0000000000000335
Basic and Clinical Research

Purpose: Conditioned medium from stem cells contains growth factors and has a promising prospect for use in regenerative medicine. In this study, the effects of marrow-derived stem cell–conditioned medium (MSC-CM) on bone regeneration after maxillary sinus floor elevation were examined in rabbits.

Materials and Methods: Rabbit bone marrow–derived stem cells (rMSCs) were cultured with MSC-CM for 48 hours, and their mobilization and proliferation were evaluated. Beta–tricalcium phosphate scaffolds were impregnated with MSC-CM and grafted in the rabbit maxillary sinus cavities. At 2, 4, and 8 weeks after transplantation, sections of the newly formed bone were evaluated histologically and by immunohistochemical staining.

Results: MSC-CM increased the migration and proliferation of rMSCs and the early bone regeneration in rabbit sinus. Cell proliferation and vascularization were increased at 2 weeks after implantation of grafts impregnated with MSC-CM compared to controls, indicating that MSC-CM is effective at the early phase of bone regeneration.

Conclusion: MSC-CM is a promising novel therapeutic agent to promote bone regeneration after maxillary sinus floor elevation.

Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Reprint requests and correspondence to: Wataru Katagiri, DDS, PhD, 65, Tsuruma-cho, Showa-ku, Nagoya city, Aichi 466-8550, Japan, Phone: +81-52-744-2348, Fax: +81-52-744-2352, E-mail:

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