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Letters to the Editor

DNA chip-assisted diagnosis for ocular toxoplasmosis

A comment

Wiwanitkit, Viroj

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Indian Journal of Ophthalmology: Mar–Apr 2012 - Volume 60 - Issue 2 - p 164
doi: 10.4103/0301-4738.94071
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Dear Editor,

I read the recent publication on DNA chip-assisted diagnosis for ocular toxoplasmosis with a great interest.[1] I accept that this report might imply the usefulness of DNA-chip technology in diagnosis of toxoplasmosis. However, there are some concerns in laboratory medicine for this report. First, although Basu et al., mentioned that validation of the DNA chip was already done there is no data on its diagnostic properties (sensitivities, specificity, accuracy, predictive value, etc.). Second, although the use of DNA chip helps provide diagnosis on multiple foci at a single time it can increase the error in the diagnosis.[2] Finally, I would like to conclude that the complete data on the properties as well as cost effectiveness of the DNA-chip approach is still required before implementation of this test for routine usage.

1. Basu S, Sharma S, Kar S, Das T. DNA chip-assisted diagnosis of a previously unknown etiology of intermediate uveitis-Toxoplasma gondii Indian J Ophthalmol. 2010;58:535–7
2. Pounds SB. Estimation and control of multiple testing error rates for microarray studies Brief Bioinform. 2006;7:25–36
© 2012 Indian Journal of Ophthalmology | Published by Wolters Kluwer – Medknow