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Letters to Editor

Sabouraud dextrose agar for the diagnosis of fungal keratitis

Wiwanitkit, Viroj

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Indian Journal of Ophthalmology: Nov–Dec 2010 - Volume 58 - Issue 6 - p 549-550
doi: 10.4103/0301-4738.71684
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Dear Editor,

I read the recent publication on diagnosis of fungal keratitis with great interest.[1] Das et al. concluded that “This study recommends use of blood agar or chocolate agar for diagnosis of both bacterial and fungal keratitis in situations with limited resources.”[1] Omitting sabouraud dextrose agar (SDA) seems to be a challenge. In microbiology, SDA is used as a standard for fungus study.[2] The use of few samples in this study should be carefully considered. In addition, some fungi, especially for Histoplasma spp. cannot be successfully cultured via blood or chocolate agars.[34]

1. Das S, Sharma S, Kar S, Sahu SK, Samal B, Mallick A. Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? Indian J Ophthalmol. 2010;58:281–6
2. Odds FC. Sabouraud(’s) agar J Med Vet Mycol. 1991;29:355–9
3. Procop GW, Cockerill FR 3rd, Vetter EA, Harmsen WS, Hughes JG, Roberts GD. Performance of five agar media for recovery of fungi from isolator blood cultures J Clin Microbiol. 2000;38:3827–9
4. Roberts GD, Washington JA 2nd. Detection of fungi in blood cultures J Clin Microbiol. 1975;1:309–10
© 2010 Indian Journal of Ophthalmology | Published by Wolters Kluwer – Medknow