There are several reports in literature, from different continents of the world, describing the prevalence of bacterial, fungal and parasitic pathogens in ulcerated corneas. [1–6] With the exception of a few population-based studies, the majority of these reports, such as those from south Florida, Nepal, Bangladesh, Ghana, and India, have primarily evaluated predisposing factors and causative agents of microbial keratitis in patients seen in the hospital. The number of patients in these studies has been less than 500 seen over a period of less than two years. Similarly, several publications on the management and treatment outcomes from various parts of the world are confined to certain groups of organisms in a limited number of patients with microbial keratitis.
In order to determine the impact of various epidemiological patterns, diagnostic methods and treatment strategies on the outcome of infective keratitis, results from studies employing standard procedures over a considerable period of time in a large number of patients would be most informative. At the L.V. Prasad Eye Institute, Hyderabad, India, every patient who reports to the cornea clinic with a stromal infiltrate in the cornea undergoes a standard protocol of clinical evaluation, diagnostic investigation, and therapeutic regimen, and all clinical and microbiological data is collected systematically.
The purpose of this study was to evaluate data pertaining to 5897 cases of presumed microbial keratitis investigated at this hospital over a period of 10 years and five months. We determined the factors predisposing to bacterial, fungal, Acanthamoeba and mixed infections, identified the causative agents prevalent, and analyzed the treatment outcome in patients with microbial keratitis.
Materials and Methods
A search of the computerized corneal ulcer database showed that 5897 clinically suspected cases of infectious keratitis had undergone microbiological investigation at this referral eye care center between February 1991 and June 2001. All these cases were defined clinically as ‘corneal ulcers’, following observation of an epithelial defect overlying a stromal infiltrate as seen on slit-lamp biomicroscopic examination. Among the 5897 cases, the medical and the microbiology data of 3563 culture-proven cases of bacterial, fungal, Acanthamoeba, and mixed keratitis were reviewed to study the demographic features, possible predisposing factors, duration of symptoms, prior therapy received, seasonal variation and laboratory results. Treatment outcome was analyzed in all patients except those with mixed infections and patients lost to follow-up.
At presentation to the cornea services of this institute, information pertaining to demographic features, duration of symptoms, risk factors and occupational status was documented for every suspected case of infectious keratitis according to a detailed protocol. Cornea evaluation was carried out by a cornea specialist using a slit-lamp biomicroscope and findings were recorded in a predesigned format. Detailed diagrammatic documentation of the ulcer was done and recorded on a daily basis. Treatment regimen, response to treatment and final outcome were recorded in all cases.
Following clinical examination, patients were subjected to microbiological investigations as per the institutional protocol described earlier.
The bacterial and fungal isolates were identified up to the species level using standard microbiological procedures.11 The smear and culture results were recorded in the predesigned format along with clinical details and captured in the corneal ulcer database which is maintained systematically. All analysis projected in this study is derived from this database.
The standard protocol used for the treatment of our patients is described in detail in an earlier publication which reported microbial keratitis in an elderly population seen at this institute from February 1991 until June 1995. The treatment protocol has remained unchanged since then for bacterial and fungal keratitis, however, we have adopted combined therapy with polyhexamethylene biguanide (0.02%) and chlorhexidine (0.02%) for Acanthamoeba keratitis since August 1996. Surgical mode of treatment included tissue adhesive application with bandage contact lens, penetrating keratoplasty, evisceration, whenever applicable. Treatment outcome at the end of three months or at the completion of treatment (whichever was earlier) was considered for analysis.
Student's t test was applied to compare the mean values of demographic factors such as age. The chi square test was used for comparison of proportions. The odds ratio (OR) with 95% confidence interval (CI) was employed to assess the relative risk of patients with trauma and agriculture-related occupation developing microbial keratitis.
Of the 5897 clinically suspected cases of infectious keratitis, 4087 (69.3%) were males and 1810 (30.7%) were females, the overall male to female ratio of patients being 2.25:1. Laboratory evidence of microbial infection was established in 3563 (60.4%) of 5897 cases whose corneal scrapings were subjected for smears and culture. The mean (± standard deviation) age was 41.20 (± 20.36) years in patients with bacterial keratitis (1849, 51.9%), 30.90 (± 15.28) years in patients with fungal keratitis (1360, 38.2%), and 34.45 (± 12.54) years in patients with Acanthamoeba keratitis (86, 2.4%), indicating a relatively increased occurrence of corneal infections (irrespective of the etiological agent) in the middle age group. The seasonal variation in the occurrence of all (including mixed) bacterial, fungal and Acanthamoeba keratitis is as depicted in Fig. 1.
Unilateral ulcer cases included 1789 right eyes and 1737 left eyes. Thirty-seven patients had bilateral infection accounting for 3600 affected eyes. Since both eyes of patients with bilateral infection revealed identical organisms, the occupational status, possible risk factors, duration of symptoms, prior medication, and laboratory parameters were analyzed taking into account 3563 patients and not eyes.
The occupations of patients [Table 1] were classified as outdoor (agriculture and manual labor), and indoor (desk job and household). More number of patients with fungal, Acanthamoeba (pure cultures) and polymicrobial keratitis (bacteria and fungus; bacteria and parasite) were found to be involved in agriculture-related activities (P < 0.001) as compared to other occupations; this feature was not evident in patients with pure bacterial keratitis and in cases where fungus and Acanthamoeba coexisted. Odds ratio (OR) revealed that patients involved in agriculture-based activities were 1.33 times (CI 1.16-1.51) at a greater risk of developing microbial keratitis.
The potential predisposing ocular factors identified in patients are shown in Table 2. Between the three etiological groups (pure cultures), the association of trauma was more pronounced for fungal and parasitic keratitis as compared to bacterial (P < 0.001). Overall, patients with ocular trauma were 5.33 times (CI 6.41-6.44) at a greater risk of developing microbial keratitis.
Patients with outdoor occupation had higher prevalence of keratitis due to trauma as compared to the patients engaged indoors. This observation was significant for bacterial (P < 0.001), fungal (P < 0.001) and Acanthamoeba (P = 0.02) keratitis when all culture-positive trauma and non-trauma cases were considered. In keratitis of pure or polymicrobial origin, physical agents were the most frequent sources of corneal injury than the other two (P < 0.001) as depicted in Fig. 2. Among the systemic factors documented in 296 patients, diabetes mellitus was more frequently noted in keratitis of both pure and polymicrobial etiology, accounting for 69.2% (205/296) cases.
Among the 3563 patients, 1945 (54.6%) were treated with antimicrobial agents and corticosteroids topically elsewhere, prior to their presentation to our cornea services [Table 3]. When retrospectively analyzed it was observed that in 945 (48.6%) patients the antimicrobial agents received were partly or completely in agreement with the type of the microbial agent (bacterial or fungal) causing the infection as proven by culture. Most patients, however, had received the medications in less than optimum dosage.
Overall, greater number of patients had sought medical help at our institute with duration of symptoms less than one month (2977) than those with symptoms longer than one month (405) as shown in Fig. 3 (P < 0.001). One thousand two hundred and fifty-two (0.06%) of 2977 patients had visited the institute within one week of onset of symptoms. On the whole, lower socioeconomic group patients (non-paying) consisted of a greater segment of the patients with microbial keratitis (3255/5897, 55.1%) as well as with positive cultures (2050/3563, 57.5%).
Direct microscopic examination of corneal scrapings detected microbes in 2884 (80.9%) of 3563 culture-positive cases. Overall, culture was positive for bacteria in 2115 (59.3%), for fungi in 1598 (44.8%) and Acanthamoeba in 118 (3.3%) of all cases (pure and polymicrobial cases). The smears revealed bacteria in 62.5% (1325/2115), fungi in 94.6% (1511/1598) and Acanthamoeba in 85.6% (101/118) of the cases. The sensitivity and specificity of each of the staining techniques employed in the detection of bacteria, fungi and Acanthamoeba are given in Table 4. On analysis of matching smear and culture results, Gram stain was accurate in only 45.7% of the corneal scrapings from 2115 patients with bacterial keratitis (pure and mixed). Among the 2334 culture-negative cases, smears were positive for microorganisms in 739 (31.7%) cases revealing bacteria in 417 (17.9%), fungus in 298 (12.8%), Acanthamoeba in 19 (0.8%) and both bacteria and fungus in five (0.2%) eyes. These cases being culture-negative were not analyzed in this study.
Of 3563 cases of microbial keratitis, 3295 (92.5%) revealed pure growth of either bacteria (1849, 51.9%), fungi (1360, 38.2%) or Acanthamoeba (86, 2.4%). Polymicrobial infection was seen in 268 (7.5%) cases. Of the 37 cases that presented with bilateral infection, 34 cases demonstrated pure bacterial growth, two had pure fungal growth, and one had mixed infection of both bacteria and fungus, both eyes of each of these patients revealing similar organisms.
More than one bacterium (two or more) was isolated from 350 cases resulting in 2511 bacterial isolates. Among the bacterial isolates, 2062 (82.1%) were gram-positive and 449 (17.9%) were gram-negative. The different bacterial and fungal species isolated are listed in Table 5 and  respectively. Propionebacterium (19, 0.8%) and Peptostreptococcus (seven, 0.3%) species were the only anaerobes recovered in this series. The antibiotic susceptibility data of the bacterial isolates is beyond the scope of this study and is published elsewhere. Overall, 1648 fungal isolates were recovered from culture of corneal scrapings (50 patients had more than one isolate). Of these, 1635 (99.2%) were molds and 13 (0.8%) were yeasts.
All patients were started on medical therapy initially, however, 46.6% of the patients required surgical intervention as shown in Table 7. Overall treatment outcome in bacterial, fungal, and Acanthamoeba keratitis patients is shown in Table 8. Significantly more number of patients required surgical treatment in fungal keratitis compared to bacterial and Acanthamoeba keratitis.
A variety of factors determine clinical outcome in microbial keratitis and the epidemiological patterns vary from one country to the other and in different geographical areas in the same country. A comprehensive data is important to develop appropriate diagnostic and therapeutic strategies. This study reports the experience with 3563 culture-positive non-viral microbial keratitis patients based in southern India. The data reported here is expected to be useful in all areas of the world where fungal keratitis is relatively more prevalent and is commonly considered in the differential diagnosis of microbial keratitis.
The male preponderance in this series was observed not only in the overall clinically suspected cases of microbial keratitis but also in culture-proven cases of microbial keratitis (male:female:2.25:1, 2.24:1 respectively). Though both sexes develop corneal ulcers more commonly in the middle decades of life, a significant male preponderance has been reported by most previous studies including those in children and elderly patients. Considering the predominant predisposing factor of trauma in all types of microbial keratitis (bacterial – 46.6%, fungal – 81.9%, Acanthamoeba – 95.5%) the probable reason for male preponderance is obvious. Ocular trauma was significantly more associated with outdoor occupation in this series.
More than half of the patients with culture-proven microbial keratitis (54.6%) had visited a physician prior to presentation at this institute and nearly half (48.6%, Table 3) of them had received antimicrobial agents that were appropriate, albeit on lower dosage, for the microbial agent involved. Therefore, we believe that despite the patient being on prior antimicrobial therapy, microbiological investigation may succeed in establishing etiological diagnosis in at least 50% of the patients. Traditional medicine or home remedy was used by only 0.4% of our patients compared to 37.3% of the patients in the study from Madurai. The urban location of our institute in contrast to the semi-urban location of the institute at Madurai may account for this difference. While use of plant extracts has been reported from rural Malawi, Africa by Courtright et al., it is fortunately not common in areas undergoing urbanization.
It is interesting to note that a majority of our patients presented within one month of onset of symptoms, 42% of whom came within one week. This indicates easy availability of transport to patients and is in contrast to the situation in other developing countries such as Nepal where 19.3% of the patients took longer than one month to reach the hospital for treatment. Transport facilities and access to healthcare systems are important issues in the developing countries and our analysis points at optimum availability in the area catered by this institute.
Direct microscopic examination of corneal scrapings provides rapid diagnosis and forms the basis for instituting initial antimicrobial therapy which may be modified later according to culture reports. An accurate smear diagnosis therefore becomes important in achieving optimum treatment outcome. The detection of fungi and Acanthamoeba was much higher in the smears than it was for bacteria [Table 4]. The detection rate for bacteria (Gram stain) was reduced by 10.9% when a correlation of the presence of similar bacteria in smears and cultures was made. We recently analyzed the utility of Gram stain in the diagnosis of early and advanced bacterial keratitis wherein the sensitivity was found to be 36.0% and 40.0% respectively. The low sensitivity was attributed by us to the use of antibiotics prior to presentation at this institute by nearly 50% of the patients. The sensitivity of Gram stain in the diagnosis of bacterial keratitis, as reported by other authors (Asbell et al. – 67%, Dunlop et al. – 62%), is close to the overall sensitivity noted in this study (56.6%) which dropped on correlation of presence of similar bacteria in smears and cultures (45.7%).
Microorganisms were isolated in 60.4% of the 5897 cases of presumed microbial keratitis. This figure is close to many other reports but is lower than the reports from Nepal (80%) and from Bangladesh (81.7%). The protocol of culture techniques followed in this study and the procedure of sample inoculation directly in the clinic leaves virtually no scope for role of laboratory-related reasons for low yield in culture. Patient-related causes such as prior antimicrobial therapy probably have a significant role to play, as has been suggested by Srinivasan et al.
A majority of our patients (3295/3563, 92.5%) had corneal infection by a single agent, the most common being bacterial (1849/3563, 51.9%). Bacterial keratitides were predominantly caused by gram-positive bacteria. However, unlike other studies from Asia and Africa where infections by Streptococcus pneumoniae were most common; in our study, Staphylococcus epidermidis-related bacterial keratitis predominated. A review of literature showed that most of the studies from developed countries such as the USA (except southern USA) and Australia listed S. epidermidis or coagulase-negative staphylococci as the leading cause of bacterial keratitis. It is not clear whether the tendency to consider S. epidermidis or coagulase-negative staphylococci as a normal commensal of the conjunctiva may have led to underreporting in some of the studies. Nevertheless, the criteria to determine the significance of a positive culture from corneal scrapings appeared similar across most of these studies. Considering the fact that S. epidermidis forms the commonest commensal of the extraocular surfaces, it is highly probable that these organisms invade corneal tissues when compromised by antimicrobial and/ or corticosteroid therapy or trauma. The higher incidence of S. pneumoniae keratitis in Madurai compared to this series remains inexplicable since both these studies are from southern India. The strong association of chronic dacryocystitis with S. penumoniea-related microbial keratitis is well known but the database in this study was not adequate to determine the frequency of concomitant sac pathology in our patients. It is possible that a larger number of patients with dacryocystitis were present in studies with predominant S. pneumoniae infection.
A high prevalence of fungal keratitis caused by filamentous fungi in warmer climates has been widely reported. All cases (pure and polymicrobial) were considered together in this series; fungi were isolated in 1598 (44.8%) patients, a frequency similar to that reported from Madurai. Some of the fungal isolates could not be definitely identified due to lack of characteristic spores [Table 6] in the medium used at our center for culturing fungus (Sabouraud dextrose agar, potato dextrose agar). Difficulty in speciation of fungi owing to lack of sporulation has been faced by other investigators as well. Attempts were not made in this study to use spore-enhancing media for fungal isolates on a routine basis, which probably would have helped in speciation of some of the unidentified isolates.
The overall incidence of Acanthamoeba keratitis (3.3%) was low in this study although the number of affected patients was large (118). Only one patient had worn contact lenses (0.8%). In contrast to the literature from developed countries, where contact lens wear emerges as a great risk factor for developing infectious keratitis, it accounted for only 42 out of 3563 (1.2%) cases in this series of which the majority (36/42, 85.7%) were bacterial. No patient among a series of 33 cases of Acanthamoeba keratitis, recently reported from south India, had worn contact lenses. Concomitant infection with bacteria (0.8%) and fungi (0.1%) was quite rare in patients with Acanthamoeba keratitis. Diagnosis based on initial smear examination of corneal scraping was most rewarding in calcofluor white stained smears by fluorescence microscopy.
A significantly larger number of patients (691/1360, 50.8%) with fungal keratitis required surgical intervention compared to bacterial (799/1849, 43.2%) and Acanthamoeba (15/86, 17.4%) keratitis thus indicating a poor response to treatment in fungal keratitis compared to bacterial and Acanthamoeba keratitis (P < 0.05). This study shows that although bacterial and Acanthamoeba keratitis can be treated effectively, the treatment of fungal keratitis remains a challenge.
The authors acknowledge the help of Ms. Rishita Nutheti for the statistical analysis of the data.
1. Liesegang TJ, Forster RK. Spectrum of microbial keratitis in south Florida Am J Ophthalmol. 1980;90:38–47
2. Upadhyay MP, Karmacharya PC, Koirala S, Tuladhar N, Bryan LE, Smolint G, et al Epidemiologic characteristics, predisposing factors, and etiologic diagnosis of corneal ulceration in Nepal Am J Ophthalmol. 1991;111:92–9
3. Katz NN, Wadud SA, Ayazuddin M. Corneal ulcer disease in Bangladesh Ann Ophthalmol. 1983;15:834–7
4. Hagan M, Wright E, Newman M, Dolin P, Johnson G. Causes of suppurative keratitis in Ghana Br J Ophthalmol. 1995;79:1024–8
5. Srinivasan M, Gonzales CA, George C, Cevallos V. Epidemiology and aetiological diagnosis of corneal ulceration in Madurai, south India Br J Ophthalmol. 1997;81:965–71
6. Bharathi MJ, Ramakrishna R, Vasu S, Meenakshi, Palaniappan R. Aetiological diagnosis of microbial keratitis in south India – A study of 1618 cases Ind J Med Microbiol. 2002;20:19–24
7. Gonzales CA, Srinivasan M, Whitcher JP, Smolin G. Incidence of corneal ulceration in Madurai District, south India Ophthalmic Epidemiol. 1996;3:159–66
8. Erie JC, Nevitt MP, Hodge DO, Ballard DJ. Incidence of ulcerative keratitis in a defined population from 1950–1988 Arch Ophthalmol. 1993;111:1665–71
9. Jones DB, Liesgang TJ, Robinson NM. Cumitech 13 Laboratory diagnosis of ocular infections. 1981 Washington DC American Society for Microbiology
10. Kunimoto DY, Sharma S, Garg P, Gopinathan U, Miller D, Rao GN. Corneal ulceration in the elderly in Hyderabad, south India Br J Ophthalmol. 2000;84:54–9
11. Balows A, Hausler WJ, Herman KL, Isenberg HDShadomy HJ Manual of Clinical Microbiology. 19916th edn Washington, DC American Society for Microbiology
12. Sharma S, Garg P, Rao GN. Patient characteristics, diagnosis and treatment
of non-contact lens related Acanthamoeba keratitis Br J Ophthalmol. 2002;84:1103–8
13. Sharma S, Kunimoto DY, Garg P, Rao GN. Trends in Antibiotic Resistance of Corneal Pathogens: Part I. An Analysis of Commonly Used Ocular Antibiotics Indian J Ophthalmol. 1999;47:95–100
14. Sharma S, Kunimoto DY, Rao TN, Garg P, Rao GN. Trends in antibiotic resistance of corneal pathogens: Part II. An analysis of leading bacterial keratitis isolates Indian J Ophthalmol. 1999;47:101–109
15. Ormerod LD, Hertzmark E, Gomez DS, Stabiner RG, Schanzlin DJ, Smith RE. Epidemiology of microbial keratitis in southern California. A multivariate analysis Ophthalmology. 1987;94:1322–33
16. Kunimoto DY, Sharma S, Reddy MK, Gopinathan U, Jyothi J, Miller D, et al Microbial keratitis in children Ophthalmology. 1998;105:252–7
17. Courtright P, Lewallen S, Kanjaloti S, Dighton D. Traditional eye medicine use among patients with corneal disease in rural Malawi Br J Ophthalmol. 1994;78:810–2
18. Sharma S, Athamanathan SNema HV, Nema N. Diagnostic procedures in infectious keratitis. Chapter 17 Diagnostic procedures in ophthalmology. 2002 New Delhi Jaypee brothers medical publishers (P) Ltd.:232–53
19. Sharma S, Kunimoto DY, Gopinathan U, Athmanathan S, Garg P, Rao GN. Evaluation of corneal scraping smear examination methods in the diagnosis of bacterial and fungal keratitis: A survey of eight years of laboratory experience Cornea. 2002;21:643–7
20. Asbell P, Stenson S. Ulcerative keratitis: Survery of 30 years' laboratory experience Arch Ophthalmol. 1982;100:77–80
21. Dunlop AA, Wright ED, Howlader SA, Nazrul I, Husain R, McClellan K, et al Suppurative corneal ulceration in Bangladesh: A study of 142 cases examining the microbiological diagnosis, clinical, and epidemiological features of bacterial and fungal keratitis Aust NZ J Ophthalmol. 1994;22:105–10
22. Carmichael TR, Wolpert M, Koornhob HJ. Corneal ulceration at an urban African hospital Br J Ophthalmol. 1985;69:920–6
23. McClellan KA, Bernard PJ, Billson FA. Microbial investigation in keratitis at the Sydney eye hospital Aust NZ J Ophthalmol. 1989;17:413–16
24. Aasuri MK, Reddy M, Sharma S, Rao GN. Co-occurrence of penumococcal keratitis and dacryocystitis Cornea. 1999;18:273–76
25. Thomas PA. Mycotic keratitis – an underestimated mycosis J Med Vet Mycology. 1994;32:235–56
26. Cohen EJ, Fulton JC, Hoffman CJ, Rapuano CJ, Laibson PR. Trends in contact lens associated ulcers Cornea. 1996;15:566–70
27. Wilhelmus KR. Review of clinical experience with microbial keratitis associated with contact lens The CLAO J. 1987;13:211–14
28. Bharathi JM, Srinivasan M, Ramakrishnan R, Meenakshi R, Padmavathy S, Lalitha PN. A study of the spectrum of Acanthamoeba keratitis: A three year study at a tertiary care referral center in South India Indian J Ophthalmol. 2007;55:37–42
Source of Support: Nil
Conflict of Interest: None declared.