Reduced severity of SARS-CoV-2 infection by Kappa variant of interest (B.1.617.1) in a healthcare worker post-vaccination from Gujarat, India : Indian Journal of Medical Research

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Reduced severity of SARS-CoV-2 infection by Kappa variant of interest (B.1.617.1) in a healthcare worker post-vaccination from Gujarat, India

Sahay, Rima Rakeshkumar1; Shete, Anita M.1; Sapkal, Gajanan N.2; Deshpande, Gururaj R.2; Shah, Arti D.4; Kumar, Sanjay3; Abraham, Priya; Yadav, Pragya D.1,*

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Indian Journal of Medical Research: May–Jun 2022 - Volume 155 - Issue 5&6 - p 575-577
doi: 10.4103/ijmr.ijmr_1390_21
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After the year long wait for the COVID-19 vaccine, India had initiated the vaccination against COVID-19 from January 16, 2021. Two vaccines, namely COVAXIN™ (BBV152, Bharat Biotech, India) and Covishield (ChAdOx1 nCoV-19, Oxford-AstraZeneca, UK manufactured by Serum Institute of India), are administered as part of the vaccine roll-out programme in India1. Post-vaccination breakthrough cases are now being reported at various time points all over the world including India2-4. Experts across the globe are trying to evaluate the COVID-19 vaccine effectiveness in the real world outside the clinical trial setup. Here, we present a case of SARS-CoV-2 infection in a healthcare worker post-COVID-19 vaccination (ChAdOx1 nCoV-19), having the Kappa variant of interest (VOI) B.1.617.1.

A 41 yr old male, doctor by profession, presented to a tertiary care hospital in Vadodara district, Gujarat, India, in March 2021 with symptoms of dry cough and mild fever of two days duration. There was no history of breathlessness, abdominal discomfort, loss of smell, headache, neurological symptoms or other significant complaints and no history of clinically significant underlying comorbidities. The patient was a healthcare worker involved in the management of COVID-19 patients admitted to his hospital. None of his family members had similar complaints. He was vaccinated with ChAdOx1 nCoV-19 and was administered the second dose one week before the onset of the symptoms. On examination, he was febrile, vital parameters were stable and SpO2 was 99 per cent. His chest computerized tomography (CT) scan showed severity index of 0/25 with a few subcentimetric and enlarged peritracheal and perivascular lymph nodes which were not radiologically significant.

His clinical specimens [throat swab (TS) and nasal swab (NS)] were obtained and tested positive for SARS-CoV-2 by real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) (N gene-16, RdRP gene-16, E gene-16)5. He was admitted to the hospital, kept under isolation and managed symptomatically. On admission, his haemoglobin was 14.6 gm/dl, normal total leucocyte counts and platelet counts. His random blood sugar levels were 142 mg/dl, normal coagulation profile, liver and kidney function test. The acute phase markers were normal including serum ferritin (117 ng/ml), D-Dimer (268 ng/ml), lactate dehydrogenase was (355 U/l) and interleukin-6 (0.50 pg/l). The patient remained admitted to the hospital in isolation ward till complete resolution of his symptoms (7 days) and was followed up on outpatient department basis subsequently for a period of eight weeks.

The TS, NS and serum samples were referred to the Indian Council of Medical Research–National Institute of Virology (ICMR-NIV), Pune, for confirmation and also for genomic sequencing. The study was approved by the Institutional Biosafety Committee and Institutional Human Ethics Committee of ICMR-NIV, Pune, under the project entitled ‘Molecular epidemiological analysis of SARS-CoV-2 circulating in different regions of India’ (20-3-18N).

The serum sample was screened for anti-IgG against the spike (S1) protein, receptor-binding domain (RBD) and nucleocapsid (N) protein (Lab care diagnostics, Mumbai, India) of SARS-CoV-2 using ELISA with optical density (OD) cut off of 0.26. Anti-IgG antibodies were detected by both the assays with the OD of 0.725 (1:3200 titre) for S1-RBD and 0.408 (1:50 titre) for N protein. The serum was also positive for anti-SARS-CoV-2 IgG antibodies by whole antigen SARS-CoV-2 human IgG antibody ELISA with O.D of 0.311 (cut-off 0.2) and P/N value of 3.247.

To characterize the SARS-CoV-2, next-generation sequencing (Illumina, California, USA) was performed as described earlier8. Reference-based mapping was done with Wuhan Hu-1 (accession number: NC_045512.2) using CLC genomics workbench 20.0.4 (Qiagen, USA), which retrieved 99.79 per cent of the SARS-CoV-2 genome. The B.1 lineage carries D614G mutation (aspartic acid is replaced by glycine at the 614th position of the spike protein) whereas sequences of B.1.617.1 pangolin lineage showed spike mutation with double mutation belonging to G clade [as per the Global Initiative on Sharing All Influenza Data (GISAID) classification-]. The double mutation refers to specific changes that are denoted by E484Q (glutamate is replaced by glutamine at the 484th position of the spike protein) and L452R (substitution of leucine with arginine at the 452nd position). However, the non-synonymous changes were also observed in spike protein including E154K (glutamate is replaced by lysine at the 154th position of the spike protein), P681R (proline is replaced by ariginine at the 681th position of the spike protein) and Q1071H (glutamine is replaced by histidine at the 1071th position of the spike protein) (Figure).

Nucleotide variations in the clinical sample of the case were observed using Base Variant analysis of the CLC Genomic Workbench 20.0.04 in comparison to the Wuhan Hu-1 (accession number: NC_045512.2). The transitions are marked with black coloured lines; transversions are marked with red-coloured lines and deletions with blue coloured lines. The amino acid changes specific to the Kappa variant of investigation B.1.617.1 in the spike protein region are mentioned in green. Nucleotide and amino acid changes are marked with single letter denotion. ‘/’ indicates the replacement sign.

The genomic surveillance has been instrumental in the detection of VOI/variants of concern (VOCs). Among these cases, VOC, Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1.617.2) and Gamma (P.1) had already been detected in the country910. The emergence of the B.1.617 lineage created a grave public health problem in India with a second wave in India. B.1.617 lineage evolved further to generate sub-lineages B.1.617.1, B.1.617.2, B.1.617.311. Nearly 60-80 per cent clinical samples from Maharashtra State in India were reported to have the VOI B.1.617.112. However, this case was reported from Gujarat, indicating the spread of Kappa variant to other parts of India. Breakthrough cases would normally be asymptomatic to mildly symptomatic post-vaccination. However, with the presence of the new VOCs and VOIs, along with the rapid mutations in the spike protein, particularly at the amino acid positions 438-506, could make them highly infectious by increasing the transmissibility. These variants have the ability to evade natural immunity or vaccine-induced immunity, which is a serious concern.

The present case had got SARS-CoV-2 infection even after completing two doses of the vaccination. However, the patient had mild symptoms, required no oxygen support and recovered completely within seven days of the infection due to Kappa variant. This short and mild course of the illness demonstrates the effectiveness of the vaccines in preventing moderate/severe disease and mortality. Similar findings of reduced disease severity in post vaccinated individual have been reported by Gupta et al4. Sapkal et al13 have reported that vaccinated individuals contracting SARS-CoV-2 infection even due to variants show a significant boost in the neutralizing antibody (NAb) titres induced by the infection. Yadav et al14 reported that the NAb titres against B.1.617 in COVAXIN recipients which was comparable to the NAb titres of recovered naturally infected cases. A major limitation of our study was that we were unable to assess fully the effectiveness of the immune responses, especially by doing the NAb titres due to low volumes of the serum samples available at the time of infection. Further, the NGS data generated were limited to the machine specificity and sensitivity.

The findings in our study have implications for the role of vaccination in response to COVID-19 considering the infection by the new variant post-vaccination and effectiveness to prevent severity and morbidity.

Financial support & sponsorship: This study was conducted at the ICMR-NIV, Pune, under intramural funding of ‘COVID-19’ provided by the Indian Council of Medical Research, New Delhi.

Conflicts of Interest: None.


Authors acknowledge the technical support provided by the staff of Maximum Containment Facility, ICMR-NIV, Pune, including Dr Rajlaxmi Jain, - Pranita Gawande, Shrimati Ashwini Waghmare, Shrimati Triparna Majumdar, Shrimati Savita Patil and Mr Abhinendra Kumar.


1. Ministry of Health and Family Welfare Government of India COVID-19 Vaccine:Operational guidelines;December, 2020 Available from: accessed on April 1, 2021
2. Keehner J, Horton LE, Pfeffer MA, Longhurst CA, Schooley RT, Currier JS, et al. SARS-CoV-2 infection after vaccination in health care workers in California N Engl J Med 2021 384 1774 5
3. CNBS News DC:About 5,800 'breakthrough infections'reported in fully vaccinated people Available from: accessed on April 16, 2021
    4. Gupta N, Kaur H, Yadav PD, Mukhopadhyay L, Sahay RR, Kumar A, et al. Clinical characterization and genomic analysis of samples from COVID-19 breakthrough infections during the second wave among the various states of India Viruses 2021 13 1782
    5. Choudhary ML, Vipat V, Jadhav S, Basu A, Cherian S, Abraham P, et al. Development of in vitro transcribed RNA as positive control for laboratory diagnosis of SARS-CoV-2 in India Indian J Med Res 2020 151 251 4
    6. Ella R, Reddy S, Jogdand H, Sarangi V, Ganneru B, Prasad S, et al. Safety and immunogenicity of an inactivated SARS-CoV-2 vaccine, BBV152:Interim results from a double-blind, randomised, multicentre, phase 2 trial, and 3-month follow-up of a double-blind, randomised phase 1 trial Lancet Infect Dis 2021 21 950 61
    7. Sapkal G, Shete-Aich A, Jain R, Yadav PD, Sarkale P, Lakra R, et al. Development of indigenous IgG ELISA for the detection of anti-SARS-CoV-2 IgG Indian J Med Res 2020 151 444 9
    8. Yadav PD, Gupta N, Nyayanit DA, Sahay RR, Shete AM, Majumdar T, et al. Imported SARS-CoV-2 V501Y. V2 variant (B.1.351) detected in travelers from South Africa and Tanzania to India Travel Med Infect Dis 2021 41 102023
    9. Yadav PD, Nyayanit DA, Sahay RR, Sarkale P, Pethani J, Patil S, et al. Isolation and characterization of the new SARS-CoV-2 variant in travellers from the United Kingdom to India:VUI-202012/01 of the B.1.1.7 lineage J Travel Med 2021 28 taab009
    10. Press Information Bureau, Government of India Genome sequencing by INSACOG shows variants of concern and a novel variant in India Available from: accessed on April 1, 2021
    11. Cherian S, Potdar V, Jadhav S, Yadav P, Gupta N, Das M, et al. SARS-CoV-2 spike mutations, L452R, T478K, E484Q and P681R, in the second wave of COVID-19 in Maharashtra, India Microorganisms 2021 9 1542
    12. Hindustan Times. Double mutant'most common variant now:India's genome data Available from: 618512310963.html accessed on April 15, 2021
    13. Sapkal GN, Yadav PD, Sahay RR, Deshpande G, Gupta N, Nyayanit DA, et al. Neutralization of Delta variant with sera of Covishield™vaccinees and COVID-19-recovered vaccinated individuals J Travel Med 2021 28 taab119
    14. Yadav PD, Sapkal GN, Abraham P, Ella R, Deshpande G, Patil DY, et al. Neutralization of variant under investigation B.1.617 with sera of BBV152 vaccinees Clin Infect Dis 2021 74 366 8
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