The suction induced epidermal graft is flimsy and translucent which is transported with the help of glass slides, acetate sheets, spatula, syringe barrel, fine dressing gauze, gloved finger, or petrolatum-soaked sheet. These methods are time-consuming, and uncurling and spreading out the graft is required after harvesting the graft. The transparent glue (cyanoacrylate glue, Healex spray, fibrin glue) is used for uncurling the graft for handy use and ease in its transportation. However, the glue renders the graft more translucent leading to difficulty in distinguishing the epidermal surface after excision of the blister, if the epidermal surface is not marked [Figure 1a-e]. Moreover, the surface identification is very crucial for the success of epidermal grafting. Keeping this in mind, a novel confusion-free strategy is advised for blister harvesting and its transportation.
On completion of the suction procedure, under asepsis, blister roof and its margin is painted sparingly (not heavily) with cyanoacrylate glue. Following this, a small piece of Micropore adhesive tape /patch (opaque, clean, not sterile but can be sterilized with ethylene oxide gas and disinfected with spray of povidone-iodine, medical spirit, and sodium hypochlorite) was made to stick (patch) on the blister rooftop. Alternative to this nonsterile tape, a small cut piece of antiseptic handyplast/band-aid can be used. After this, a blister is harvested from the edge with the tip of a hypodermic needle. The surfaces of the harvested blister graft are easily identified, and it can be handled and transported without confusion [Figure 2a-d]. The tape sticker of small size is not removed from the graft surface as it does not hamper the grafting procedure and the graft uptake. Thus, a Micropore tape sticker on the glued blister roof is a novel technique for easy identification of the graft surface that helps in confusion-free transportation and grafting[Figure 3a-d]. The incidence of infection is not significant in using Micropore adhesive tape on clean wound. As an alternative to Micropore, the graft roof (in tensed, deflated state) can be marked easily with a surgical pen marker with 2 consequent English letters or Arabic numbers to avoid confusion about the graft surface.
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Conflicts of interest
There are no conflicts of interest.
I acknowledge Dr. Bushra khan, M.D. (Dermatology) for her help in editing the text of this article
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