Analysis of Hypersensitivity in Fragrance Series by Patch Testing : Indian Dermatology Online Journal

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Analysis of Hypersensitivity in Fragrance Series by Patch Testing

Periyasamy, Mohan Kumar; Sekar, Shanmuga C.; Rai, Reena

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Indian Dermatology Online Journal 10(6):p 657-662, Nov–Dec 2019. | DOI: 10.4103/idoj.IDOJ_490_18
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Allergic contact dermatitis (ACD) is an inflammatory disorder, which occurs as a result of repeated contact with an allergen, leading to the rapid activation of T cells and further release of cytokines.[1] In the sixth century, Aetius Amidenus, a physician to the Byzantine court first used the word “eczema”. Eczema may present clinically as scaling, clustered papulovesicles, associated with erythema, pruritus and fissuring. It is caused by a different variety of external and internal factors.[2] Patch testing is considered as the gold standard and only reliable method to identify the contact allergens.[3]

Cosmetics is defined as the substances which are intended to be poured, rubbed, sprinkled or sprayed on skin, applied to a normal or previously sensitized skin for cleansing, promoting attractiveness, beautifying, or to alter the appearance of the human skin or body.[4] Cosmetics and toiletries are being used by most of the people for hygiene and personal care of the body to enhance the attractiveness of consumers, get pleasant smell, protection and masking the defects present in the skin.[5] Cosmetics are complex mixtures made up of preservatives, perfumes, emulsifiers, stabilizing agents, various types of lipids, alcohols and so on.[6]

Fragrances are the products obtained naturally or produced synthetically. Natural fragrances, such as balsams, concretes, essential oils and absolutes are available, with a few animal products such as musk, civet and ambergris, which also can be manufactured synthetically.[7] Masking fragrances are used mainly in topical medicaments and cosmetic products by labeling them as “fragrance free”. But they contain original fragrances.[8] The purpose of this study is to detect all the fragrance allergens which cause ACD.

Materials and Methods

This is an open-label prospective observational study conducted from March 2017 to July 2018 in a tertiary care hospital. All patients of age above 18 years who attended our dermatology outpatient department with the history of using various topical cosmetic products such as perfumes, detergent soap, after shave lotion, moisturizers, facial makeup creams, with dermatitis lesions over face, neck, axilla and hands were included in the study. Age below 18 years, who have not used any cosmetic products, who are on systemic corticosteroids, immunosuppressants, pregnant, lactating females, were excluded from the study.

The study was reviewed and approved by an institutional ethical committee, and all patients gave their voluntary informed consent to participate in this study. Detailed history regarding duration, occupation, systemic illness was obtained and recorded [Table 1]. Patch testing with fragrance series [Figure 1] was done by using the standard technique, that is, 0.1 ml of each antigen was placed in an aluminum Finn chamber, mounted on an adhesive tape [Figure 2], which was applied over the back of all the patients [Figure 3]. A total of 42 patch test antigens [Table 2] are present in the fragrance series obtained from chemotechnique diagnostics, AB Sweden. The results were interpreted on days 2 and 4 as recommended by International Contact Dermatitis Research Group (ICDRG) criteria [Table 3].

Table 1:
Demographic data of all 27 patients
Figure 1:
Fragrance series antigens (Chemotechnique diagnostics, AB Sweden)
Figure 2:
Patch testing: Antigens are placed in aluminum Finn chambers mounted over an adhesive tape
Figure 3:
Patch test antigens applied over the back of patients
Table 2:
Fragrance series antigens (Chemotechnique Diagnostics, AB Sweden)
Table 3:
Interpretation of Patch Test Results (ICDRG Criteria)


A total of 27 patients were included in this study and patch testing was done with fragrance series. Of them, 12 (44.4%) were males and 15 (55.5%) were females; the mean age was 43 years (range 18-68 years). The mean duration of symptoms was 12.5 months (range 1-24 months). The most common site of involvement was hands [Figure 4], which was observed in 19 (70.3%) patients [Table 4], followed by neck [Figure 5] in 12 (44.4%) patients.

Figure 4:
Hyperpigmented scaly lesions over the hands
Table 4:
Location of dermatitis lesions
Figure 5:
Erythema and scaling seen over the neck

The most commonly seen occupational groups with dermatitis features were housewives 6 (22.2%) and office workers 6 (22.2%) patients [Table 5]. The most commonly used category of cosmetic products by these patients were talcum powders by 13 (48.1%) patients [Table 6] followed by scented soaps in 12 (44.4%) patients.

Table 5:
Various occupational groups presented with dermatitis
Table 6:
Categories of the cosmetic products used by the patients

Out of the 27 patients, 8 (29.6%) were atopic individuals and 19 (70.3%) were non-atopic individuals. Of the 27 patients, 23 (85.18%) patients showed at least one antigen positivity, and 4 (14.8%) patients were negative to all antigens. The most frequent allergen to become positive in this study is fragrance mix II [Figure 6] in eight (29.6%) patients [Table 7], followed by cinnamic aldehyde and cinnamic alcohol in seven (25.9%) patients each [Figure 7].

Figure 6:
Patch test: Same patient shown in Figure 4, showed positive reaction to fragrance mix II
Table 7:
Frequency of fragrance allergen positivity
Figure 7:
Patch test: Same patient shown in Figure 5, showed positive reaction to cinnamic alcohol and cinnamic aldehyde


ACD is one of the commonest examples of type IV hypersensitivity reaction which usually affects the previously sensitized persons. The contact allergens are very small molecules which are able to penetrate deeper layers of the skin and produce sensitization.[2] Fragrances are the most common cause of allergic reactions to cosmetics. Fragrances can enter into the body through nose into lungs, upper airways, ingestion, skin, and it can cause irritation of eyes, throat and nose, headaches, dizziness, forgetfulness and easy fatigability.[9]

In our study, females outnumbered males in fragrance hypersensitivity reactions by nearly 10%. The fact that there is a high prevalence of fragrance allergy in women than men could be due to the frequent use of skin care, personal hygiene and face care products by the female population. In this study, housewives and office workers are the occupational groups who developed fragrance allergy most frequently, which is seen in six (22.2%) patients in each occupational group. DeGroot et al.[7] found that fragrance allergy from perfumes, deodorants and aftershave lotions is commonly encountered in Swedish college students.

In this study, most commonly 19 (70.3%) patients had lesions over the hands, and next frequent site was over the neck in 12 (44.4%) patients. Our findings correlate well with the studies conducted by Santucci and Malten;[7] they found that hand dermatitis was the most common presentation with 41% and 52% positivity, respectively, because of frequent contact of the fragrance products such as soaps, fairness creams, shampoos and topical medications with hands before application at various sites of the body.[7]

In this study, the most common category of cosmetic products used were talcum powders by 13 (48.1%) patients and scented soaps by 12 (44.4%) patients. An observational study found that the skin care products such as lotions, creams were the cosmetic categories which were blamed for the positive reactions in patch testing in 37% of patients; 30% of positive cases had used personal care products and 13% used deodorants and antiperspirants.[5] According to Cornelis et al., soaps were used by 87% of the people, 82% people used toothpaste, shampoos were used by 80%, deodorants and antiperspirants by 61%, talcum powder and body spray were used by nearly 45% of the study population.[5]

In this study, we found that the most frequent allergen to show positivity was fragrance mix II in eight (29.6%) patients. The second most common allergens were cinnamic aldehyde and cinnamic alcohol in seven (25.9%) patients, which is followed by Geranium oil Bourbon and Lavender absolute in five (18.5%) patients. Our observations are similar to a study conducted by Johansen et al.: who stated that the most common allergens are fragrance mix II and balsam of peru.[10] Santucci et al. identified 54 out of 92 patients were sensitive to fragrance mix II.[7]

Various studies have been conducted in various countries to know the incidence of fragrance allergy, but only a few studies are available for our Indian skin type. This is because the fragrance allergy is usually not reported to the dermatologists by the patients. In USA, the most frequent sensitizer was fragrance mix II. In Netherlands, isoeugenol is followed by oak moss absolute. In a European study, they found that oak moss absolute is the most frequently positive fragrance allergen followed by isoeugenol.[10]

In this study, a total of 27 patients were included with dermatitis. Of them, 8 (29.6%) patients were known atopic and 19 (70.3%) patients were non-atopic individuals. But Katsarma et al.[10] stated that more than 50% of ACD patients were with atopic diathesis in their study. Caress and Maria et al.[11] also concluded that the fragrance-induced ACD is most commonly seen in atopic eczema cases.

In this study, we did not experience any adverse events or intolerability to patch testing. All the 27 patients came for regular follow-up and also for the readings taken on day 2 and day 4. Once the patient is diagnosed as a case of ACD by patch testing, pamphlets were issued regarding the awareness of how to avoid contact with the particular allergens. Cosmetics are not very safe as claimed by the manufacturing companies, and it may contain many fragrance allergens. ACD induced by cosmetics was more prevalent in urban people than rural because of the health awareness and attitude towards cosmetics in urban people.[12]


Patch testing is a simple method to diagnose the causative agent of ACD. It shortens the time lapse from the first visit to final diagnosis and increases the period of remission, which in turn reduces the cost of treatment. We conclude that fragrance mix II is an important marker to find out fragrance allergy. Hand dermatitis is the most common presentation in patients with fragrance allergy. Perfumed talcum powders, soaps and perfumes are the leading source of sensitization to fragrance allergens producing fragrance allergy.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.


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Allergic contact dermatitis; cosmetics; fragrances; hand dermatitis; patch testing

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