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TH1/TH2-mediated colitis induced by adoptive transfer of CD4+CD45RBhigh T lymphocytes into nude mice

Kanai, Takanori MD, PhD1,*; Kawamura, Takahiro MD, PhD1; Dohi, Taeko MD, PhD2; Makita, Shin MD, PhD1; Nemoto, Yasuhiro MD1; Totsuka, Teruji MD, PhD1; Watanabe, Mamoru MD, PhD1

doi: 10.1097/01.MIB.0000197237.21387.mL
Original Articles

Background: Transfer of CD4+CD45RBhigh T cells from normal donors to SCID/Rag-1, 2-deficient mice, which lack T and B cells, leads to the development of a TH1-mediated inflammatory bowel disease (IBD)-like syndrome characterized by extensive mononuclear cell infiltrates and epithelial cell hyperplasia. Because it is well known that B cells are also involved in a multitude of mechanistic pathways in human IBD, this study attempts to establish a new model of colitis in nude mice.

Methods: We transferred CD4+CD45RBhigh T cells into athymic nude mice, which lack thymus-dependent T cells but retain normal B cells, to establish and investigate a B cell-involving chronic colitis model. As a control, CD4+CD25 T cells were also used.

Results: Mice reconstituted with CD4+CD45RBhigh but not CD4+CD25 T cells developed a wasting disease, with severe infiltrates of B cell aggregates as well as T cells, macrophages, and dendritic cells into the colon and elevated levels of interferon-γ, tumor necrosis factor-α, interleukin (IL)-4, IL-5, and IL-10, by 7 weeks after T cell transfer. Furthermore, the infiltrated lamina propria B cells in colitic nude mice consisted predominantly of massive aggregated immunoglobulin (Ig) M- and scattered IgG-positive cells, but not IgA-positive cells. In contrast, mice reconstituted with CD4+CD45RBhigh and CD4+CD45RBlow did not develop wasting disease or colitis.

Conclusions: Collectively, the power of the colitis model induced by the adoptive transfer of CD4+CD45RBhigh T cells into nude mice is that one can investigate the roles of TH2-type cells and B cells in a regulatory T cell-depleted condition.

1 Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo

2 Department of Gastroenterology, Research Institute, International Medical Center of Japan, Tokyo

*Reprints: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan


Received 10 September 2005; Accepted 9 November 2005

This study was supported in part by grants-in-aid for Scientific Research, Scientific Research on Priority Areas, Exploratory Research and Creative Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology; Grant sponsor: Japanese Ministry of Health, Labor and Welfare; Grant sponsor: Japan Medical Association; Foundation for Advancement of International Science; Grant sponsor: Yakult Bio-Science Foundation; Grant sponsor: Research Fund of the Mitsukoshi Health and Welfare Foundation; Grant sponsor: Okawa Intractable Disease Research Foundation; Grant sponsor: Ooyama Health Science Foundation; Grant sponsor: Japan Health Sciences Foundation.

© Crohn's & Colitis Foundation of America, Inc.
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