1Department of Liver Surgery, Center of Hepato‐Pancreato‐Biliary Surgery, The First Affiliated Hospital, Sun Yat‐sen University, Guangzhou, China
2Department of Radiation Oncology, The First Affiliated Hospital, Sun Yat‐sen University, Guangzhou, China
3Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat‐sen University, Guangzhou, China
4Center for Translational Medicine, The First Affiliated Hospital, Sun Yat‐sen University, Guangzhou, China
5Clinical Trials Unit, The First Affiliated Hospital, Sun Yat‐sen University, Guangzhou, China
6Cancer Center, The First Affiliated Hospital, Sun Yat‐sen University, Guangzhou, China
Correspondence Zhenwei Peng, Department of Radiation Oncology, The First Affiliated Hospital, Sun Yat‐sen University, Guangzhou 510080, China. Email: [email protected]
Funding information This work was supported by National Natural Science Foundation of China (No. 82072029); the top-notch young talents of the national “Ten Thousand Programme” (No.2019–04); “The three and three projects” from The First Affiliated Hospital, Sun Yat-sen University (No. Y70310); and the Guangdong Basic and Applied Basic Research Foundation (No. 2021A1515011182 and 2021A1515012496).
Abbreviations: CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats; DNA‐PKcs, DNA‐dependent protein kinase catalytic subunit; DSB, double‐strand break; FPKM, fragments per kilobase of transcript per million mapped reads; GFP, green fluorescent protein; HCC, hepatocellular carcinoma; HR, homologous recombination; IHC, immunohistochemistry; IR, ionizing radiation; IR‐R, ionizing radiation–resistant; KD, knockdown; m7G, N7‐methylguanosine; METTL1, methyltransferase 1; mRNA, messenger RNA; NCG, NOD/ShiltJGpt‐Prkdcem26Cd 52Il2rgem26Cd22/Gpt; NHEJ, nonhomologous end‐joining; OE, overexpression; OS, overall survival; PDX, patient‐derived xenograft; PFS, progression‐free survival; qRT‐PCR, quantitative reverse transcription polymerase chain reaction; RNC, ribosome nascent‐chain complex; SB, sleeping beauty; SYSUCC, Sun Yat‐sen University Cancer Center; SYSUFAH, The First Affiliated Hospital, Sun Yat‐sen University; TRAC‐seq, tRNA m7G reduction and cleavage sequencing; tRNA, transfer RNA; VMAT, Volumetric Modulated Arc Therapy; WB, western blot; WT, wild‐type.
Junbin Liao, Yang Yi, and Xin Yue contributed equally to this work.
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