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Bruenger F. W.; Stover, Betsy J.; Stevens, W.
Health Physics: November 1971
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Homogenates of livers from beagles injected with 239Pu(IV) in citrate buffer and sacrificed at times ranging from 7 to 1539 days after injection were separated by differential centrifugation. Significant concentrations of plutonium were found in the subcellular particles, the cytosol and the connective tissue. Concentrations in the mitochondrial-lysosomal fractions and microsomal fractions were the highest. Separation of the heavy mitochondria) fraction and of the light mitochondrial-lysosomal fraction by density gradient centrifugation showed that 239Pu and acid-phosphatase are associated with protein of the same density. Most of the plutonium in the cytosol was bound to ferritin. Smaller fractions of plutonium were found in the very high molecular weight components (probably lipofuscin) and some with an unidentified low molecular weight material. Pure Pu-containing ferritin was separated by gel electrophoresis and by ultracentrifugation. The high concentration of plutonium in the microsomal fraction resulted in large part from contamination by ferritin which was heavily loaded with iron.

©1971Health Physics Society