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Biological Dosimetry Network in Africa

Establishment of a Dose-Response Curve Using Telomere and Centromere Staining

Soumboundou, Mamadou1; Nkengurutse, Innocent2; Dossou, Julien3; Colicchio, Bruno4; Djebou, Catherine3; Gadji, Macoura5; Houenon, Germain3; Dem, Ahmadou6; Dedjan, Alexandre3; Diarra, Mounibé7; Adjibade, Rachad3; Finot, Francis8; Hempel, William9; Dieterlen, Alain4; Jeandidier, Eric10; Rodriguez-Lafrasse, Claire11; M’kacher, Radhia9

doi: 10.1097/HP.0000000000001102

Purpose: Biological dosimetry, based on the relationship between the absorbed dose after exposure to ionizing radiation and the frequency of scored aberrations, has been and continues to be an important tool for estimating the dose after exposure. Dicentric chromosomes are considered to be the most specific and sensitive aberration related to radiation exposure. Here, we established the dose-response curve following in vitro irradiation of circulating lymphocytes from healthy donors from three African countries after scoring unstable chromosomal aberrations.Materials and methods: Blood samples from 16 African donors were exposed to various doses (0 to 4 Gy) using an X-RAD320 x-ray system with a maximum photon energy of 250 kV at a dose rate of 0.1 Gy min−1. Blood lymphocytes were cultured for 48 h, and chromosomal aberrations were scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined. Results: No dicentric chromosomes were found after the analysis of 2,669 first-division metaphases before in vitro exposure. We established a linear-quadratic dose-response curve based on the frequency of dicentric and ring chromosomes and calculated double-strand breaks, taking into account all scored aberrations.Conclusion: The generation of a specific dose-response curve for African donors will allow the practice of precise biological dosimetry in these countries. This work is the first step towards realizing an African biodosimetry network and the establishment of a biological dosimetry laboratory, which could play a major role in the application of radioprotection norms.

1Laboratoire de Biophysique, UFR-Santé Thiès, Hôpital pour Enfants de Diamniadio, Sénégal

2Institut National de Santé Publique (INSP), Bujumbura, Burundi

3Laboratoire de Biologie Appliquée (LARBA)/Unité de Recherche en Carcinogénèse et Morphologie Humaines (URCMH) de l’Ecole Polytechnique de l’Université d’Abomey-Calavi, Bénin

4IRIMAS, Institut de Recherche en Informatique, Mathématiques, Automatique et Signal, Université de Haute-Alsace, Mulhouse, France

5Laboratoire Hématologie, Université Cheikh Anta DIOP-Dakar, Sénégal

6Institut de Cancérologie Marie-Curie, Département Oncologie, Université Cheikh Anta DIOP-Dakar, Sénégal

7Laboratoire de Physique-Pharmaceutique, Université Cheikh Anta DIOP-Dakar-Sénégal

8Genevolution Laboratory, Porcheville, France

9Cell Environment, DNA Damage R&D, Paris, France

10Service de Génétique Médicale, Groupe Hospitalier de la Région de Mulhouse Sud-Alsace, Mulhouse, France

11Laboratoire de Radiobiologie Cellulaire et Moléculaire IPNL, Université de Lyon, Faculté de Médecine Lyon-Sud, Oullins, France.

The authors declare no conflicts of interest.

For correspondence contact Radhia M’kacher, Cell Environment, DNA Damage R&D, Rue des Pyrénées, 75020 Paris, France, or email at

(Manuscript accepted 6 March 2019)

Online date: June 7, 2019

© 2019 by the Health Physics Society