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ORIGINAL RESEARCH

Transdermal Progesterone Cream for Vasomotor Symptoms and Postmenopausal Bone Loss

LEONETTI, HELENE B. MD; LONGO, SANTO MD; ANASTI, JAMES N. MD

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Progesterone from diosgenin, extracted from Mexican yams, is identical to the natural progesterone of the human ovary or placenta. 1 Transdermal progesterone has been used cosmetically for over 20 years; however, recently those preparations have been used as alternatives to traditional hormone replacement therapy (HRT). Because of its organic origin and lack of side effects, use of transdermal progesterone cream has increased dramatically. Studies using cell culture and animal models suggest that progesterone might function as an osteotrophic hormone. 2 Anecdotal evidence and human noncontrolled trials suggested daily use of transdermal progesterone cream might increase lumbar spine bone mineral density in postmenopausal women and improve overall well-being. Lee noted, in reviewing his personal files, an increase in spinal bone mineral density in 63 of 100 menopausal women treated daily with 20 mg of transdermal progesterone cream. 3 He also reported an increase in well-being and excellent compliance, owing to ease of use and lack of side effects. Recent data also suggest that natural progesterone might have theoretical advantages over oral medroxyprogesterone acetate based on lipid profiles and coronary vascular experiments in primates. 4,5

We designed a randomized, double-masked, placebo-controlled trial to investigate the effectiveness of transdermal progesterone cream for controlling vasomotor symptoms and preventing menopausal bone loss.

Materials and Methods

We recruited postmenopausal women over a 6-month period, advertising in local newspapers and sending letters to physicians under a protocol approved by the St. Luke's Hospital Institutional Review Board. Subjects included were within 5 years of menopause, had serum FSH exceeding 40 IU/L, and were free of hormonal therapy for at least 1 year before beginning the study.

Each subject had a complete history and physical examination, including a Zung Self-Rating Depression Scale questionnaire (Zung Scale). 6 Initial blood studies collected were fasting lipids, including total cholesterol, low-density lipids, high-density lipids, triglycerides, TSH, FSH, and serum chemistries. Subjects had bone mineral density measured in the lumbar spine and hip, using dual energy x-ray absorptiometry. Bone mineral density measurements were done with a single Hologic Quantitative Digital Machine (Hologic MDM, Waltham, MA) and recorded in grams per square centimeter with a coefficient of variation of 1.2%. After we reviewed screening examinations, serum levels, and bone mineral density, subjects were randomly assigned to progesterone cream or placebo. The progesterone was compounded with mixed tocopherol cream to contain 20 mg of progesterone per quarter teaspoon. Placebo was mixed tocopherol cream alone and was indistinguishable from the active compound. The formulator of placebo and active cream (Transition for Health, Portland, OR) shipped the jars of cream directly to our research department, which assigned medicine using computer-generated random numbers and issued the cream directly to the subjects. Investigators and subjects were masked to treatment arms until final analysis of the study. Subjects were instructed to apply the cream daily, rotating the application site between the upper arms, thighs, and breasts. To ensure adequate dietary supplementation, participants were instructed to take multivitamins and 1200 mg of calcium daily, and to record symptom diaries. “Hot flashes” were defined as symptomatic sweating and perceived alterations in body temperature bothersome enough to cause changes in behavior (sleep disturbances, clothing changes, or altering room temperature). At the end of each week, subjects recorded whether hot flashes increased, remained the same, improved, or stopped. Subjects also documented any vaginal bleeding, disturbing mood swings, rash development, or miscellaneous symptoms. Compliance was determined by estimating the amount of cream remaining at the time of each follow-up visit.

Subjects returned for evaluation at 4, 8, and 12 months, at which time symptom diaries were reviewed and studies were done. At 4 months, repeat fasting lipid profiles were drawn, and the Zung Scale was administered. During the 12-month visit, repeat lipids, chemistry profile, TSH, and Zung Scale were done. Dual x-ray absorptiometry scans were repeated during the 12-month visit on the same machine as initial scans.

Statistical analyses used Sigma Stat (Jandel Scientific, San Rafel, CA), with P < .05 considered statistically significant. Differences between groups were compared using two-tailed t test and χ2 as appropriate. Based on the findings of Lee, 3 we calculated a sample size of 40, assuming 50% of the treatment and 20% of the placebo group would show increases exceeding 1.2% in bone mineral density (α = 05; 1 −β = 80).

Results

One hundred seven women were screened initially, but two were excluded for having FSH values under 40 IU/L, and three for having contraindicated medical problems. Of 102 women enrolled, 12 were unable to finish the protocol, two developed rashes (one placebo and one progesterone cream), two for hospitalizations unrelated to the study, four owing to poor compliance issues (one lost to follow-up and three for failure to keep appointments) and four for medication compliance concerns (determined by estimating over 15% of doses missed), leaving 43 transdermal progesterone-treated women and 47 placebo subjects for analysis.

Initial values of the treatment and placebo groups did not differ with respect to age, race, time since last menstrual period, body mass index (BMI), FSH, lipid profiles, Zung Scale scores, vasomotor symptoms, lumbar spine bone mineral density, and total hip bone mineral density (Tables 1 and 2). Mean post-treatment BMI decreased slightly in the placebo and transdermal progesterone groups, but did not reach statistical significance. Lipid profiles appeared unaltered in the progesterone treatment group and were not significantly different from the control group. There was a small but significant difference in the pretreatment and post-treatment femoral neck bone mineral density between groups. A small, nonsignificant decrease in mean bone mineral density was noted in both groups at the post-treatment dual x-ray absorptiometry scan. The change over initial bone mineral density of the lumbar spine, femoral neck, and total hip were similar in each group (Table 2). The number of subjects who showed an increases of bone mineral density of over 1.2% was similar in both groups (Table 2).

Table 1
Table 1:
Initial Values
Table 2
Table 2:
Comparison of Pretreatment and Post-treatment Bone Mineral Density

Eleven women in the placebo group and ten in the treatment group had initial Zung Self-Rating Depression scores suggestive of mild depression (index score over 50 and under 60). At the end of 1 year, overall scores decreased slightly in both groups; however, the numbers of individuals noted to have mild depression by Zung Self-Rating Depression score decreased to three in the treatment group and four in the control group, which were not statistically significant.

Symptom diaries showed vaginal spotting in eight subjects in the progesterone group (P < .01). One biopsy found a proliferative endometrium disorder and the other seven had tissue insufficient for diagnosis. In all cases, the spotting was self-limited and resolved within 1–2 days. Twenty-six of 47 controls and 30 of 43 treated subjects had vasomotor symptoms. After the 4-month visits, five women reported improvements in vasomotor symptoms in the control group. In the treatment group, 14 women reported improvements in their vasomotor symptoms and 11 reported complete resolution of those symptoms. None of the subjects noted increases in those symptoms. Those findings did not change at subsequent visits, and most had maximum relief after the first month. The treatment group had 25 of 30 (83%) improvements or resolutions of vasomotor symptoms compared with five of 26 (19%) in the control group which was statistically significant (P < .001). Other symptoms recorded in the diaries were difficult to quantify, thus not analyzed.

Discussion

Vasomotor symptoms occur within 3 months of menopause in 70% of women, and half will continue to have hot flashes during the first 5 years after the cessation of menses. Vasomotor flushing is the most bothersome complaint of menopause, a poorly understood phenomenon that is the main reason women seek HRT and remain compliant with it. For over 40 years, estrogens have been the mainstay of treatment of hot flashes, but several studies showed progestins to be effective as well. Bullock noted the incidental finding that women who had medroxyprogesterone acetate treatment for endometrial cancer had significant relief of hot flashes. 7 Morrison, using depot medroxyprogesterone acetate, 8 and Schiff, using 10 mg of oral medroxyprogesterone acetate, 9 found responses as high as 87% in treated groups, compared with 15% in placebo groups. The mechanism of action of progestins in controlling hot flashes is unknown. We noted a similar effect in our menopausal women who had vasomotor symptoms, with 83% improvement in hot flashes with progesterone cream and 19% improvement with placebo.

Cooper and colleagues, using a direct time-resolved fluoroimmunoassay, found significantly higher progesterone levels in women using progesterone cream than those using placebo. 10 However, progesterone levels in the transdermal group were a third of those obtained with 5 days of oral, micronized progesterone. We did not measure progesterone levels in our women because we were interested in the clinical effect of progesterone cream. The occurrence of vaginal bleeding and resolution of vasomotor symptoms reported in the progesterone-treated group suggested a systemic effect, which might be an unexplained, bioactive progesterone availability undetected by conventional assays.

Results of studies of progestogen effects on bone mineral density in humans are mixed. Prior et al, in runners with amenorrhea, and McNeely, in postmenopausal women, showed a benefit on bone mineral density by oral medroxyprogesterone. 11,12 Others have not seen similar positive effects on bone mineral density with progestin. 13,14 Although unable to show an increase in bone mineral density in a 1-year study on the effects of progestins in early postovariectomy subjects, Prior did show an increase in bone formation markers in the medroxyprogesterone acetate–treated group, 15 which implies that longer treatment might be necessary to increase bone mineral density. Our study parameters were based on the findings of Lee, who noted an increase in spinal bone mineral density exceeding 1.5%/year in 63 of his 100 postmenopausal women treated with 20 mg of progesterone cream. We were unable to find a similar increase of bone mineral density in our study. Our conclusion on the effect of transdermal progesterone on bone mineral density might be limited by duration and dose.

References

1. Peterson CM. Progestogens, progesterone antagonists, progesterone, and androgens: Synthesis, classification, and uses. Clin Obstet Gynecol 1995;38:813–20.
2. Prior JC. Progesterone as a bone-trophic hormone. Endocrin Rev 1990;11:386–98.
3. Lee JR. Osteoporosis reversal: The role of progesterone. Clin Nutr Rev 1990;10:384–9.
4. The Writing Group for the PEPI. Effects of hormone therapy on bone mineral density: Results from the postmenopausal estrogen/progestin interventions (PEPI) trial. JAMA 1996;276:1389–96.
5. Minshall RD, Stanczyk FZ, Miyagawa K, Uchida B, Axthelm M, Novy M, et al. Ovarian steroid protection against coronary artery hyperreactivity in rhesus monkeys. J Clin Endocrinol Metab 1998;83:649–59.
6. Zung WW, Richards CB, Short MJ, Colon DML, Guzman YF, Guevara-Ramos LM. Early detection of depression using the Zung Self-Rating Depression Scale. Arch Gen Psychiatry 1965;13:508–15.
7. Bullock JL, Massey FM, Gambrell RD Jr. Use of medroxyprogesterone acetate to prevent menopausal symptoms. Obstet Gynecol 1975;46:165–8.
8. Morrison JC, Martin DC, Blair RA, Anderson GD, Kincheloe BW, Bates GW, et al. The use of medroxyprogesterone acetate for relief of climacteric symptoms. Am J Obstet Gynecol 1980;138:99–104.
9. Schiff I, Tulchinsky D, Cramer D, Ryan KJ. Oral medroxyprogesterone in the treatment of postmenopausal symptoms. JAMA 1980;244:1443–5.
10. Cooper A, Spencer C, Whitehead MI, Ross D, Barnard GJ, Collins WP. Systemic absorption of progesterone from Progest cream in postmenopausal women. Lancet 1998;351:1255–6.
11. Prior JC, Vigna YM, Barr SI, Rexworthy C, Lentle BC. Cyclic medroxyprogesterone treatment increases bone density: A controlled trial in active women with menstrual cycle disturbances. Am J Med 1994;96:521–30.
12. McNeeley SGJ, Schinfeld JS, Stovall TG, Ling FW, Buxton BH. Prevention of osteoporosis by medroxyprogesterone acetate in postmenopausal women. Int J Gynaecol Obstet 1991;34:253–6.
13. Adachi JD, Sargeant EJ, Sagle MA, Lamont D, Fawcett PD, Bensen WG, et al. A double-blind randomized controlled trial of the effects of medroxyprogesterone acetate on bone density of women taking oestrogen replacement therapy. Br J Obstet Gynaecol 1997;104:64–70.
14. Caird LE, West CP, Lumsden MA, Hannan WJ, Gow SM. Medroxyprogesterone acetate with Zoladex for long-term treatment of fibroids: Effects on bone density and patient acceptability. Hum Reprod 1997;12:436–40.
15. Prior JC, Vigna YM, Wark JD, Eyre DR, Lentle BC, Li DK, et al. Premenopausal ovariectomy-related bone loss: A randomized, double-blind, one-year trial of conjugated estrogen or medroxyprogesterone acetate. J Bone Miner Res 1997;12:1851–63.
© 1999 The American College of Obstetricians and Gynecologists