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Determination of Fetal RhD Status From Uncultured Amniocytes

Obstetrics & Gynecology: August 1996
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Objective To determine the accuracy of DNA analysis for fetal RhD status using polymerase chain reaction (PCR) on uncultured amniocytes.

Methods Three hundred forty-seven amniotic fluid (AF) samples obtained for a variety of clinical indications were tested for fetal RhD status using PCR and primers specific for the genes coding for D and Cc/Ee. Fetal RhD status was confirmed by serologic methods on neonatal cord blood obtained at the time of delivery.

Results In 346 of 347 cases (99.7%), the PCR results of AF were in agreement with the serologic RhD blood type. The single error was in an RhD-negative fetus (misidentified as RhD-positive) whose AF was contaminated with blood from an RhD-positive mother. The likelihood of an erroneous result was 0.3%.

Conclusion Polymerase chain reaction–based analysis of fetal RhD status using uncultured amniocytes and established primers appears to be highly accurate.

Address reprint requests to: G. Marc Jackson, MD, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104–4283

© 1996 The American College of Obstetricians and Gynecologists

Use of PCR and established primers on uncultured amniocytes for determination of fetal RhD status is highly accurate.