Total estrogen concentrations in amniotic fluid were determined in 9 normal and 12 complicated pregnancies. Ammonium sulfate precipitation of the conjugated and free estrogens was employed. Solutions of total estrogens were assayed fluorometrically by the Ittrich procedure. Tracer studies demonstrated that conjugated and free estrogens in amniotic fluid may be isolated in high yields by ammonium sulfate precipitation. Complicated pregnancies exemplified conditions affecting the fetus such as Rh isoimmunization and anencephaly. Lower values were noted in association with severely affected fetuses, but normal and abnormal groups were not completely differentiated.
© 1970 The American College of Obstetricians and Gynecologists