To analyze the subgingival microflora composition of inflammatory bowel disease (IBD) patients with untreated chronic periodontitis and compare them with systemically healthy controls also having untreated chronic periodontitis.
Thirty IBD patients [15 with Crohn’s disease (CD) and 15 with ulcerative colitis (UC)] and 15 control individuals participated in the study. All patients had been diagnosed with untreated chronic periodontitis. From every patient, subgingival plaque was collected from four gingivitis and four periodontitis sites with paper points. Samples from the same category (gingivitis or periodontitis) in each patient were pooled together and stored at −70°C until analysis using a checkerboard DNA–DNA hybridization technique for 74 bacterial species.
Multiple-comparison analysis showed that the groups differed in bacterial counts for Bacteroides ureolyticus, Campylobacter gracilis, Parvimonas micra, Prevotella melaninogenica, Peptostreptococcus anaerobius, Staphylococcus aureus, Streptococcus anginosus, Streptococcus intermedius, Streptococcus mitis, Streptococcus mutans, and Treponema denticola (P<0.001). CD patients had significantly higher levels of these bacteria than UC patients either in gingivitis or in periodontitis sites (P<0.05). CD patients harbored higher levels of P. melaninogenica, S. aureus, S. anginosus, and S. mutans compared with controls both at gingivitis and at periodontitis sites (P<0.05). UC patients harbored higher levels of S. aureus (P=0.01) and P. anaerobius (P=0.05) than controls only in gingivitis sites.
Our study showed that even with similar clinical periodontal parameters, IBD patients harbor higher levels of bacteria that are related to opportunistic infections in inflamed subgingival sites that might be harmful for the crucial microbe–host interaction.
aDepartament of Periodontology, Faculty of Odontology
bDepartment of Gastroenterology, Faculty of Medicine, Rio de Janeiro State University
cDepartment of Gastroenterology, Faculty of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
dDepartment of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
eDepartment of Oral Medicine, School of Dentistry, University of Washington, Seattle, Washington, USA
fDepartment of Dental Medicine, Division of Perisodontology, Karolinska Institutet, Stockholm, Sweden
Correspondence to Carlos M.S. Figueredo, PhD, Departament of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Boulevard 28 de Setembro 157, Pavilhão de Pesquisa, Vila Isabel, Rio de Janeiro 20551-030, Brazil Tel/fax: +55 212 868 8642; e-mail: email@example.com
Received July 12, 2012
Accepted September 5, 2012