CarcinogenesisAll-trans retinoic acid suppressed GES-1 cell proliferation induced by exosomes from patients with precancerous lesions by arresting the cell cycle in S-phaseGao, Leia,,*; He, Yuea,,*; Wang, Kexina; Wang, Chena; Wu, Hanhana; Hu, Anlaa; Ruan, Lianga; Bo, Qinglia; Chen, Wenjuna; Hu, Chuanlaia; Li, Lia; Zhang, Daomingb; Xia, Taob; Qian, Shiqingc; Liu, Zhiningd; Yang, Yie; Yang, Wanshuia; Wang, Huaf,,g; Zhao, QihongaAuthor Information aDepartment of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University Departments of bGastroenterology cPathology, Lujiang County People’s Hospital dDepartment of General Surgery, the Second Affiliated Hospital of Anhui Medical University Departments of ePathology fOncology, the First Affiliated Hospital of Anhui Medical University gInstitute for Liver Diseases of Anhui Medical University, Hefei, Anhui Province, China *Dr. Lei Gao and Dr. Yue He contributed equally to the writing of this article. Received 3 November 2019 Accepted 25 November 2019 Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's website (www.eurjcancerprev.com). Correspondence to Qihong Zhao, Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China.Tel/fax: +86 551 65165035. Email address: [email protected] European Journal of Cancer Prevention: January 2021 - Volume 30 - Issue 1 - p 113-119 doi: 10.1097/CEJ.0000000000000571 Buy SDC Metrics Abstract We aimed to detect the expression of specific LncRNAs in exosomes isolated from the serum of patients with precancerous lesions and to study the effect of these serum exosomes on the activity of GES-1 cells in patients with precancerous lesions, as well as the activity of all-trans retinoic acid on GES-1 cells with or without the exosomes. Exosomes were extracted from the serum of patients with precancerous lesions and normal controls. Based on our previous sequencing results, quantitative real time-PCR was used to detect differentially expressed LncRNAs. Exosomes from the serum of patients with precancerous lesions were cocultured with GES-1 cells, and 5 μM all-trans retinoic acid was added as an intervention. Changes in cell viability and expression of LncHOXA10 were observed. Compared with the blank group, the proliferation activity of GES-1 cells cocultured with exosomes derived from the serum of patients with precancerous lesions was increased (P < 0.01), the proportion of cells in S phase was increased (P < 0.05). After adding 5 μM all-trans retinoic acid, the viability of cells decreased significantly (P < 0.01), the proportion of cells in S phase decreased significantly (P < 0.05). The expression of LncHOXA10 was decreased (P < 0.05). All-trans retinoic acid can conduct its chemopreventive effects by inhibiting the expression of LncHOXA10, thereby reducing the activity of LncHOXA10 in GES-1 cells cocultured with serum exosomes from patients with precancerous lesions. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.