Institutional members access full text with Ovid®

Share this article on:

Berberine prevents 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis: a biochemical approach

Manoharan, Shanmugam; Sindhu, Ganapathy; Vinothkumar, Veerasamy; Kowsalya, Raju

European Journal of Cancer Prevention: March 2012 - Volume 21 - Issue 2 - p 182–192
doi: 10.1097/CEJ.0b013e32834c9c3c
Research Papers: Head and Neck cancer

Chemoprevention, a novel and useful approach in experimental oncology, deals with the prevention, suppression, or inhibition of carcinogenesis using natural or synthetic entities. This study evaluated the chemopreventive potential of berberine on 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Oral squamous cell carcinoma was developed in the buccal pouch of golden Syrian hamsters by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. Tumor incidence, tumor volume, tumor burden, phase I and phase II carcinogen detoxification agents, lipid peroxidation, antioxidant status, and histopathological changes were assessed in hamsters treated with DMBA alone and in DMBA+berberine-treated animals. Hundred percent tumor incidences with an imbalance in carcinogen-metabolizing enzymes and cellular redox status were observed in hamsters treated with DMBA alone. Oral administration of berberine at a dose of 75 mg/kg body weight (bw) to DMBA-treated hamsters completely prevented tumor incidence and restored the status of the above-mentioned biochemical markers. Berberine, a traditional drug from Southeast Asia, shows promising chemopreventive efficacy in hamster buccal pouch carcinogenesis.

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Tamil Nadu, India

Correspondence to Shanmugam Manoharan, PhD, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, TN 608 002, India Tel: 09442548117; fax: +914144238080; e-mail

Received May 20, 2011

Accepted July 17, 2011

© 2012 Lippincott Williams & Wilkins, Inc.