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Protective effect of Acticoa powder, a cocoa polyphenolic extract, on prostate carcinogenesis in Wistar–Unilever rats

Bisson, Jean-François; Guardia-Llorens, Maria-Alba; Hidalgo, Sophie; Rozan, Pascale; Messaoudi, Michaël

European Journal of Cancer Prevention: February 2008 - Volume 17 - Issue 1 - p 54-61
doi: 10.1097/CEJ.0b013e3280145b33
Research papers: Prostate Cancer
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The effects of Acticoa powder on prostate carcinogenesis were investigated using the N-methylnitrosourea and testosterone propionate prostate tumor model. Sixty male Wistar–Unilever rats were randomly divided in four groups of 15 rats: one control group not induced but treated with vehicle (not induced+vehicle) and three chemo-induced groups. Two weeks before prostate tumor induction and then throughout the experiment, chemo-induced rats were orally treated with Acticoa powder at 24 (chemo-induced+Acticoa powder24) or 48 (chemo-induced+Acticoa powder48) mg/kg or with vehicle (chemo-induced+vehicle), daily from Monday to Friday. Survival, body weight, food and water consumption were recorded throughout the experiment. Six rats per group were randomly killed 9 months after the prostate tumor induction for histopathological analysis of prostates. A reduction in the incidence of prostate tumors was observed for the chemo-induced+Acticoa powder48-treated group in comparison with the chemo-induced+vehicle-treated group and no tumors were observed in the chemo-induced+Acticoa powder24-treated group as in the not induced+vehicle-treated group after 9 months. The nine remaining rats per group were maintained in a long-term survival study. The life span of the chemo-induced+Acticoa powder24-treated group was significantly increased in comparison with the chemo-induced+Acticoa powder48 and the chemo-induced+vehicle-treated groups, close to the one of the not induced+vehicle-treated group. A significant reduction in the incidence of prostate tumors was also observed for the chemo-induced+Acticoa powder24 and chemo-induced+Acticoa powder48-treated groups in comparison with the chemo-induced+vehicle-treated group. In conclusion, Acticoa powder at 24 mg/kg protected rats from prostate carcinogenesis when chronically given before the initiation and promotion phases of induction.

ETAP-Ethologie Appliquée, Centre de Recherche en Pharmacologie, Cancérologie et Nutrition, Vandoeuvre-lès-Nancy, France

Correspondence to Dr Jean-François Bisson, PhD, ETAP-Ethologie Appliquée, Département de Cancérologie et de Pathologies Humaines, 13 rue du Bois de la Champelle, 54500 Vandoeuvre-lès-Nancy, France

Tel: +33 0 383 444 635; fax: +33 0 383 446 441;

e-mail: jfbisson@etap-lab.com

Received 23 August 2006 Accepted 25 October 2006

© 2008 Lippincott Williams & Wilkins, Inc.