Research papers: Gastrointestinal CancerExpression of CD147 as a significantly unfavorable prognostic factor in hepatocellular carcinomaZhang, Qinga *; Zhou, Junb *; Ku, Xiao-Minga *; Chen, Xin-Guof *; Zhang, Lic; Xu, Jinga; Chen, Guang-Shengd; Li, Qine; Qian, Fengf; Tian, Rongg; Wen, Ningh; Chen, Zhi-NanaAuthor Information aDepartment of Cell Biology and Cell Engineering Research Centre, State Key Laboratory of Cancer Biology, the Fourth Military Medical University bDepartment of Pharmacology, School of Medicine of Xi'an Jiaotong University cFirst Department of Surgery, ShaanXi Hospital of Chinese People's Armed Police Forces dDepartment of Pathology, Xijing Hospital, the Fourth Military Medical University eShaanxi Carcinoma Hospital, Xi'an fInstitute of Hepatology, General Hospital of Chinese People's Armed Police Forces gAir Force General Hospital hGeneral Hospital of PLA, Beijing, China Correspondence to Qing Zhang, 17 West Changle Street, Xi'an, Shannxi 710032, China Tel: +86 29 83374547; fax: +86 29 83226349; e-mail: [email protected] Requests for reprints to Zhinan Chen, Cell Engineering Research Centre and State Key Laboratory of Cancer Biology, the Fourth Military Medical University, 17 Changlexi Street, Xi'an 710032, China Tel: +86 29 83374547; fax: +86 29 83226349; e-mail: [email protected] Sponsorship: National Natural Science Foundation of China 39989002 and 30070842 (Z.N. Chen). *These authors contributed equally to this work. Q.Z. performed the studies and drafted the manuscript. Z.N.C., J.Z. and X.G.C. conceived of the study, and participated in the design the manuscript. J.X. and H.X. participated in the writing assistance. Q.L. participated in the statistical analysis. F.Q., G.S.C., R.T. and N.W. provided purely technical help. All authors read and approved the final manuscript. Received 3 January 2006 Accepted 16 March 2006 European Journal of Cancer Prevention: June 2007 - Volume 16 - Issue 3 - p 196-202 doi: 10.1097/01.cej.0000236245.40619.c3 Buy Metrics Abstract The aim of this study is to investigate whether the expression of CD147 could be a prognostic factor for hepatocellular carcinoma. Tissue samples from 111 hepatocellular carcinoma patients were immunohistochemically stained with anti-CD147, anti-matrix metalloproteinases-2 and anti-vascular endothelial growth factor antibodies. Tumor microvessel density was evaluated using CD34. The survival curves were estimated by Kaplan–Meier analysis and the prognostic significance of the marker was analyzed using the log-rank test. In addition, the identification of relevant prognostic factors was performed by multivariate Cox regression analysis. CD147 was mainly expressed in cancerous lesions and its expression was positively correlated with metalloproteinases-2 (P<0.0001), vascular endothelial growth factor (P<0.0001) and microvessel density CD34 (P<0.0001). Furthermore, CD147 was significantly associated with the presence of venous invasion (P=0.0013), tumor size (P<0.0001) and pTNM tumor stages (P=0.0001), as well as serum α-fetoprotein level (P<0.0001). Patients with positive expression of CD147 had poorer tumor recurrence-free survival than those with negative expression of CD147 (P<0.0001). Analyzed by a proportional hazard model, strong expression of CD147 had the highest risk ratio of recurrence among these markers (P<0.0001). The findings suggest that CD147 may be a significant independent predictor of poor survival in patients with hepatocellular carcinoma, and may be involved in tumor growth, invasion and angiogenesis in hepatocellular carcinoma. © 2007 Lippincott Williams & Wilkins, Inc.